Desmocollin-3 Antibody (765129)

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Western blot shows lysates of mouse skin tissue and mouse embryo (15 d.p.c.) tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Desmocollin‑3 Monoclonal Antibody (Catalog # MAB7265) followed by ...read more
Desmocollin‑3 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse Desmocollin‑3 Monoclonal Antibody (Catalog # MAB7265) at 25 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clone
765129
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Desmocollin-3 Antibody (765129) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-3
Arg136-Leu695
Accession # P55850
Specificity
Detects mouse Desmocollin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 20% cross-reactivity with recombinant human Desmocollin-3 is observed and no cross-reactivity with recombinant mouse (rm) Desmocollin-1 or rmDesmocollin-2 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
DSC3
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB7265 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmocollin-3 Antibody (765129)

  • Cadherin family member 3
  • CDHF3
  • CDHF3HT-CP
  • desmocollin 3
  • Desmocollin3
  • Desmocollin-3
  • desmocollin-4
  • DSC
  • DSC1
  • DSC2
  • DSC3
  • DSC4
  • DSC4desmocollin-3
  • HT-CP

Background

DSC-3 (Desmocollin [Greek for "glue-that-binds"]-3) is a 95-110 kDa member of the Ca++-dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including keratinized and nonkeratinized epithelia of the urinary bladder, vagina, oral cavity and skin. DSC-3 serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, in the extracellular space, DSC-3 forms both homotypic and heterotypic interactions with DSG-1 in-trans, and binds to the cytoskeleton intracellularly via plakophilin-3. Mature mouse DSC-3 is a 760 amino acid (aa) type I transmembrane glycoprotein. The mature molecule contains a 560 aa extracellular region with five cadherin domains (aa 136-691), and a 179 aa cytoplasmic domain that possesses three utilized Ser phosphorylation sites. There is one splice variant that shows an eight aa substitution for aa 831-895. Over aa 136-695, mouse DSC-3 shares 79% and 93% aa identity with human and rat DSC-3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Desmocollin-3 Antibody (MAB7265) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB7265:
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ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Desmocollin-3 MAB7265
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reviewed by:
Qi Miao
ICC Mouse 06/22/2017
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Summary

ApplicationImmunocytochemistry
Sample Testedskin keratinocytes
SpeciesMouse

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Diseases for Desmocollin-3 Antibody (MAB7265)

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Recent Reviews

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Qi Miao
06/22/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol DSC3
Entrez
Uniprot