Desmocollin-2 Antibody (7G6)

Immunohistochemistry-Paraffin: Desmocollin-2 Antibody (7G6) [NBP2-53378] - Formalin-fixed, paraffin-embedded human Skin stained with Desmocollin-2 Antibody (7G6).

Immunohistochemistry-Paraffin: Desmocollin-2 Antibody (7G6) [NBP2-53378] - Formalin-fixed, paraffin-embedded human Skin stained with Desmocollin-2/3 Monoclonal Antibody (7G6).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: 7G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Desmocollin-2 Antibody (7G6) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54519)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human Desmocollin-2 extracellular domain
• Localization: Cell surface
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DSC2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Western Blot 0.5-1.0 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Reactivity Notes

Others not known.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Desmocollin-2 Antibody (7G6)

• ARVD11
• Cadherin family member 2
• CDHF2
• CDHF2DGII/III
• desmocollin 2
• Desmocollin2
• Desmocollin-2
• Desmocollin-3
• Desmosomal glycoprotein II
• desmosomal glycoprotein II/III
• Desmosomal glycoprotein III
• DG2
• DSC2
• DSC3DKFZp686I11137

Background

Desmocollin 2 is encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11. Alternative splicing results in two transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Desmocollin-2 Antibody (NBP2-53378)(1)

Publication using NBP2-53378

• Franzén Boger M, Benhach N, Hasselrot T et al. A topical rectal douche product containing Q-Griffithsin does not disrupt the epithelial border or alter CD4+ cell distribution in the human rectal mucosa Scientific reports 2023-05-09 [PMID: 37161022] (Immunohistochemistry, Human)

Bioinformatics

• Gene Symbol: DSC2
• Uniprot:
  • Q02487(Human)