| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-46853SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-46852SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-16971-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22520-50ul | |||
![Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP1-46853-img0003.jpg "Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain")
![Immunohistochemistry: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN1 in human fetal brain](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-NBP1-46853-img0004.jpg)
![Western Blot: RCAN1 Antibody [NBP1-46852] - Western blot analysis of human skeletal muscle tissue (A) using RCAN1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP1-46852-img0006.jpg "Western Blot: RCAN1 Antibody [NBP1-46852] - Western blot analysis of human skeletal muscle tissue (A) using RCAN1 antibody at 2 ug/ml.")
![Immunohistochemistry: RCAN1 Antibody [NBP1-46852] - Immunohistochemical analysis of RCAN1 in human fetal brain.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-NBP1-46852-img0003.jpg)
![Western Blot: RCAN1 Antibody [NBP1-46852] - Analysis of RCAN1 in human fetal brain.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP1-46852-img0004.jpg)
![Western Blot: RCAN1 Antibody [NBP2-86769] - RCAN1 antibody - middle region validated by WB using Mouse Brain at 1:500.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP2-86769-img0006.jpg "Western Blot: RCAN1 Antibody [NBP2-86769] - RCAN1 antibody - middle region validated by WB using Mouse Brain at 1:500.")
![Western Blot: RCAN1 Antibody [NBP2-86769] - WB Suggested Anti-RCAN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP2-86769-img0001.jpg)
![Western Blot: RCAN1 Antibody [NBP2-86769] - RCAN1 antibody - middle region validated by WB using MOUSE OSTEOCLASTS at 1:1000.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP2-86769-img0002.jpg)
![Western Blot: RCAN1 Antibody [NBP2-86769] - WB Suggested Anti-RCAN1 Antibody. Positive Control: Lane 1: 20ug Wild type mouse, left ventricle. Primary Antibody Dilution : 1:1000. Secondary Antibody : Goat anti rabbit-HRP. Secondry Antibody Dilution : 1:5,000. Submitted by: Kathleen Gabrielson](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP2-86769-img0003.jpg)
![Western Blot: RCAN1 Antibody [NBP2-86769] - Host: Rabbit. Target Name: RCAN1. Sample Tissue: Human HepG2 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP2-86769-img0005.jpg)
![Western Blot: RCAN1 Antibody [NBP2-86769] - Host: Rabbit. Target Name: RCAN1. Sample Type: Human Fetal Muscle. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide. Primary Antibody Concentration: 1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane/lane.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP2-86769-img0004.jpg)
![Western Blot: RCAN1 Antibody (JE62-71) [NBP3-32100] - Western blot analysis of RCAN1 on different lysates with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Lane 6: Mouse brain tissue lysate (40 ug/Lane) Lane 7: Mouse thymus tissue lysate (40 ug/Lane) Lane 8: Rat cerebellum tissue lysate (40 ug/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32100) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-278202415242658.jpg "Western Blot: RCAN1 Antibody (JE62-71) [NBP3-32100] - Western blot analysis of RCAN1 on different lysates with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Lane 6: Mouse brain tissue lysate (40 ug/Lane) Lane 7: Mouse thymus tissue lysate (40 ug/Lane) Lane 8: Rat cerebellum tissue lysate (40 ug/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32100) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: RCAN1 Antibody (JE62-71) [NBP3-32100] - Immunocytochemistry analysis of Jurkat cells labeling RCAN1 with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-29202410234244.jpg)
![Flow Cytometry: RCAN1 Antibody (JE62-71) [NBP3-32100] - Flow cytometric analysis of Jurkat cells labeling RCAN1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32100, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-29202416372121.jpg)
![Western Blot: RCAN1 Antibody [NBP2-86771] - Host: Rabbit. Target Name: RCAN1. Sample Type: Fetal Kidney lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP2-86771-img0002.jpg "Western Blot: RCAN1 Antibody [NBP2-86771] - Host: Rabbit. Target Name: RCAN1. Sample Type: Fetal Kidney lysates. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry-Paraffin: RCAN1 Antibody [NBP2-86771] - Rabbit Anti-RCAN1 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue. Observed Staining: Cytoplasmic, nucleus. Primary Antibody Concentration: 1:100.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-Paraffin-NBP2-86771-img0001.jpg)
![Western Blot: RCAN1 Antibody [NBP2-86771] - Host: Mouse. Target Name: RCAN1. Sample Tissue: Mouse Testis. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP2-86771-img0003.jpg)
![Western Blot: RCAN1 Antibody [NBP3-23360] - Western blot analysis of RCAN1 expression in human Jurkat (lanes 1 & 4), mouse C2C12 (lanes 2 & 5), and rat PC12 (lanes 3 & 6). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000 and anti-RCAN1 (a.a. 132-140) at 1:500.](http://images.novusbio.com/fullsize/nbp3-23360_rabbit-rcan1-pab-19920231992819.jpg "Western Blot: RCAN1 Antibody [NBP3-23360] - Western blot analysis of RCAN1 expression in human Jurkat (lanes 1 & 4), mouse C2C12 (lanes 2 & 5), and rat PC12 (lanes 3 & 6). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000 and anti-RCAN1 (a.a. 132-140) at 1:500.")
![Immunocytochemistry/Immunofluorescence: RCAN1 Antibody [NBP3-23360] - Immunocytochemical labeling of RCAN1 in aldehyde-fixed NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-RCAN1 (C-terminus) (NBP3-23371) and anti-RCAN1 (a.a. 132-140) (NBP3-23360) antibodies (Left side). These antibodies were also used in the presence (Right side) of blocking peptide. The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23360_rabbit-rcan1-pab-199202312523631.jpg)
![Immunocytochemistry/Immunofluorescence: RCAN1 Antibody [NBP3-23371] - Immunocytochemical labeling of RCAN1 in aldehyde-fixed NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-RCAN1 (C-terminus) (NBP3-23371) and anti-RCAN1 (a.a. 132-140) (NBP3-23360) antibodies (Left side). These antibodies were also used in the presence (Right side) of blocking peptide RX3945 and RX3965, respectively. The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23371_rabbit-rcan1-pab-199202317594419.jpg "Immunocytochemistry/Immunofluorescence: RCAN1 Antibody [NBP3-23371] - Immunocytochemical labeling of RCAN1 in aldehyde-fixed NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-RCAN1 (C-terminus) (NBP3-23371) and anti-RCAN1 (a.a. 132-140) (NBP3-23360) antibodies (Left side). These antibodies were also used in the presence (Right side) of blocking peptide RX3945 and RX3965, respectively. The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: RCAN1 Antibody [NBP3-23371] - Western blot analysis of RCAN1 expression in human Jurkat (lane 1), rat PC12 (lane 2), human A431 (lane 3), and adult mouse muscle (lane 4). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000.](http://images.novusbio.com/fullsize/nbp3-23371_rabbit-rcan1-pab-189202317355216.jpg)
![Immunohistochemistry-Paraffin: RCAN1 Antibody [NBP3-16971] - Analysis in human parathyroid gland and tonsil tissues using Anti-RCAN1 antibody. Corresponding RCAN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-Paraffin-NBP3-16971-img0004.jpg "Immunohistochemistry-Paraffin: RCAN1 Antibody [NBP3-16971] - Analysis in human parathyroid gland and tonsil tissues using Anti-RCAN1 antibody. Corresponding RCAN1 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: RCAN1 Antibody [NBP3-16971] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-Paraffin-NBP3-16971-img0001.jpg)
![Immunohistochemistry-Paraffin: RCAN1 Antibody [NBP3-16971] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-Paraffin-NBP3-16971-img0002.jpg)
![Western Blot: RCAN1 Antibody [NBP2-86770] - WB Suggested Anti-RCAN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Placenta](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP2-86770-img0002.jpg "Western Blot: RCAN1 Antibody [NBP2-86770] - WB Suggested Anti-RCAN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Placenta")
![Immunohistochemistry-Paraffin: RCAN1 Antibody [NBP2-86770] - Rabbit Anti-RCAN1 / DSCR1 antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult Brain, cerebellum. Observed Staining: Cytoplasm in hepatocytes. Primary Antibody Concentration: 1:600.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-Paraffin-NBP2-86770-img0001.jpg)
![Western Blot: RCAN1 Antibody (SR1461) [NBP3-22520] - Western blot analysis using NBP3-22520 in mouse heart cell lysate.](http://images.novusbio.com/fullsize/nbp3-22520_rabbit-rcan1-mab-sr1461-57202317161815.jpg "Western Blot: RCAN1 Antibody (SR1461) [NBP3-22520] - Western blot analysis using NBP3-22520 in mouse heart cell lysate.")