RASAL1 Antibody

Images

 
Western Blot: RASAL1 Antibody [NBP1-32776] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: RASAL1 Antibody [NBP1-32776] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RASAL1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human RASAL1. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RASAL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-32776 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (85%), Rat (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RASAL1 Antibody

  • RAS protein activator like 1 (GAP1 like)
  • RASALGAP1 like protein
  • rasGAP-activating-like protein 1

Background

The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RASAL1 Antibody (NBP1-32776) (0)

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Review for RASAL1 Antibody (NBP1-32776) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-32776:
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Images Ratings Applications Species Date Details
Western Blot RASAL1 NBP1-32776
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reviewed by:
Youg Thaker
WB Human 02/10/2014
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Summary

ApplicationWestern Blot
Sample Tested293T lysate transfected with hRasal1
SpeciesHuman
Lot40030
CommentsCell lysates from 293T cells transfected with hRasal1 (lane 1) or empty vector (lane 2) for 24h.

Blocking

Blocking Details5 % BSA

Primary Anitbody

Dilution Ratio1/1000 in 2% BSA

Secondary Antibody

Secondary DescriptionGoat anti-Rabbit HRP
Secondary Manufacturer Cat#NA9340-1ML
Secondary Concentration1/5000

Details

Detection NotesECL plus (1min)

Comments

CommentsCell lysates from 293T cells transfected with hRasal1 (lane 1) or empty vector (lane 2) for 24h.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RASAL1 Antibody (NBP1-32776) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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Youg Thaker
02/10/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RASAL1
Entrez
Uniprot