RASAL1 Antibody - BSA Free

Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 inhibits the ERK1/2 pathway, but not p38 pathway. Expression of Rasal1 in T cells inhibits ERK1,2 phosphorylation. Upper and lower panels: two independent experiments where primary mouse T cells were transfected with Flag-tagged Rasal1 and were ligated with anti-CD3 for 5 min followed by blotting with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204); anti-ERK1/2, anti-ZAP-70 or anti-Flag. Flag-PLK expression served as a negative control (top panel) (n > 2). Upper right panel shows endogenous levels of Rasal1 in activated primary T cells. Lower right panel: histogram showing the inhibition of pERK relative to ERK expression. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-12544-4) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: RASAL1 Antibody [NBP1-32776] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

Western Blot: RASAL1 Antibody [NBP1-32776] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE, antibody diluted at 1:1000.

Western Blot: RASAL1 Antibody [NBP1-32776] - Protein expression was upregulated in older female crab-eating monkey brain. Image submitted by a verified customer review.

Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 inhibits in vitro anti-CD3 & dendritic cell-peptide induced T-cell activation. a Antigen (OVA323-39)-dependent activation & proliferation of DO11.10 CD4 + cells expressing Flag-tagged Rasal1 compared with control cells at different OVA peptide concentrations (5–10 ng). CTLA-Ig was used as a control. Upper inset shows expression of Flag-tagged Rasal using anti-Flag in blotting (n = 3). b Inhibition of T-cell responses by Rasal1 to increasing anti-CD3 concentrations. Upper inset: effects of Rasal1 at 48 & 72 h post-anti-CD3 activation (n = 3). c Anti-CD3-driven NFATc1/AP1 promoter activity in Jurkat T cells was inhibited by the presence of Flag-tagged Rasal1 (n = 3). d siRNA (esiRNA) knockdown of Rasal1 enhances T-cell proliferation. esiRNA were tested for their knockdown efficiency by western blot (top right panel), esiRNA1 & 2 correspond to 500 nM & 1000 nM concentrations (n = 3). e Contact time between D011.10 CD4+T-cells expressing Flag-Rasal1 (red) or empty vector (blue) & OVA peptide on bone marrow dendritic cells (BMDCs). Dot blot of T cell/DC contact duration time in presence of OVA was unaffected by Flag-tagged Rasal1 or vector transfection (n = 3). Representative plots show mean with ± SD. p-value was calculated by unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RASAL1 Antibody [NBP1-32776] - TCR complex purification & Rasal1 association with ZAP-70 in the TCR complex. a Analytic gel from tandem affinity purification (TAP) of TCR plus associated proteins captured from TCR-constituted cells (TCR complex) versus TCR-null negative control cells (control) (left panel). Chart showing the list of TCR-associated proteins identified by MS analysis (right upper panel). The structure of Rasal1 (right lower panel). b Co-precipitation of ZAP-70 with TCR from 3A9 T cells. 3A9 cells were stimulated for 10 min with anti-CD3 followed by immunoprecipitation with anti-TCR zeta & blotting with anti-ZAP-70 or anti-zeta. Lane 1: resting T cells; lane 2: anti-CD3-stimulated T cells. c Rasal1 expression is induced with anti-CD3 activation. Relative fold change expression of Rasal1 versus Rasal3 upon anti-CD3 stimulation of CD4+ & CD8 + peripheral T cells. 18 s rRNA served as a control. d Anti-CD3 induces Rasal1 translocation to the membranes of T cells. Stimulation-dependent association of Rasal1 with the membrane (upper panel) versus cytosolic Rasal1 (lower panel). Anti-actin served as a loading control. LAT was used as a marker of membrane enrichment fractionation (n = 3) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 binds to the immune cell tyrosine kinase ZAP-70. a Proximity ligation assay (PLA) of Rasal1 & ZAP-70 in Jurkat T-cells stained with DAPI, anti-Rasal1, & anti-ZAP-70. Positive PLA signal (fluorescent dots) indicates a distance of 30–40 nm between Rasal1 & ZAP-70 in anti-CD3-activated T cells, indicating their close proximity (n = 2). b Anti-Rasal1 co-precipitates ZAP-70 from primary mouse T cells. Lane 1–2 loading controls, lanes 3-4: anti-Rasal1 IP: lanes 2 & 4. Cells were stimulated with anti-CD3 ligation for 5 min vs IgG control ligation in lanes 1 & 3. c Anti-Rasal1 co-precipitates ZAP-70 from transfected Jurkat T cells. Lanes 1: nonligated (i.e., IgG); lane 2: anti-CD3 ligated T cells for 5 min. Lower panel shows similar level of ZAP-70 expression in lysates of (n = 2) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 binds to the immune cell tyrosine kinase ZAP-70. a Proximity ligation assay (PLA) of Rasal1 & ZAP-70 in Jurkat T-cells stained with DAPI, anti-Rasal1, & anti-ZAP-70. Positive PLA signal (fluorescent dots) indicates a distance of 30–40 nm between Rasal1 & ZAP-70 in anti-CD3-activated T cells, indicating their close proximity (n = 2). b Anti-Rasal1 co-precipitates ZAP-70 from primary mouse T cells. Lane 1–2 loading controls, lanes 3-4: anti-Rasal1 IP: lanes 2 & 4. Cells were stimulated with anti-CD3 ligation for 5 min vs IgG control ligation in lanes 1 & 3. c Anti-Rasal1 co-precipitates ZAP-70 from transfected Jurkat T cells. Lanes 1: nonligated (i.e., IgG); lane 2: anti-CD3 ligated T cells for 5 min. Lower panel shows similar level of ZAP-70 expression in lysates of (n = 2) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Pm
• Applications: WB, Func, ICC/IF, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RASAL1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human RASAL1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RASAL1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Knockdown Validated
  • Proximity Ligation Assay
  • Western Blot 1:500-1:3000
• Application Notes: RASAL1 antibody validated for WB from a verified customer review. Use in Proximity Ligation Assay reported in scientific literature (PMID:31641113).
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31641113). Monkey reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RASAL1 Antibody - BSA Free

• RAS protein activator like 1 (GAP1 like)
• RASALGAP1 like protein
• rasGAP-activating-like protein 1

Background

The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

&#9888; WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for RASAL1 Antibody (NBP1-32776)(2)

Publications using NBP1-32776

• M. H. S. Deurloo, S. Eide, E. Turlova, Q. Li, S. Spijker, H.-S. Sun, A. J. A. Groffen, Z.-P. Feng Rasal1 regulates calcium dependent neuronal maturation by modifying microtubule dynamics Cell & Bioscience 2024-01-21 [PMID: 38246997]
• Thaker YR, Raab M, Strebhardt K et al. GTPase-activating protein Rasal1 associates with ZAP-70 of the TCR and negatively regulates T-cell tumor immunity Nat Commun 2019-10-22 [PMID: 31641113] (PLA, KD, WB, Mouse)

Reviews for RASAL1 Antibody (NBP1-32776) (2)

Average Rating: 3.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Monkey.

reviewed by: Hui Zhang WB Monkey 02/13/2019

Summary

• Application: Western Blot
• Sample Tested: brain and spinal cord
• Species: Monkey
• Lot: 40030

Comments

• Comments: Antibody dilution 1:1000

reviewed by: Youg Thaker WB Human 02/10/2014

Summary

• Application: Western Blot
• Sample Tested: 293T lysate transfected with hRasal1
• Species: Human
• Lot: 40030

Bioinformatics

• Gene Symbol: RASAL1
• Entrez:
  • 8437(Human)
• Uniprot:
  • O95294(Human)