GPR35 Antibody - BSA Free

Western Blot: GPR35 Antibody [NBP2-24640] - Analysis of G Protein-Coupled Receptor GPR35 in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide 3) RAW using NBP2-24640 at 0.5 ug/ml and 1.0 ug/ml, respectively.

Immunohistochemistry-Paraffin: GPR35 Antibody [NBP2-24640] - Analysis of GPR35 in paraffin-embedded formalin-fixed MCF7cells using an isotype control (top left) and NBP2-24640 (bottom left, right) at 5 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GPR35 Antibody - BSA Free Summary

• Immunogen: A portion of amino acids 275-309 of human G Protein-Coupled Receptor GPR35 was used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPR35
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 0.5-1.0 ug/ml
• Control Peptide:

  GPR35 Protein (NBP2-24640PEP)

Reactivity Notes

Human reactivity reported in scientific literature (PMID:32755573).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR35 Antibody - BSA Free

• G protein-coupled receptor 35
• GPR35
• G-protein coupled receptor 35
• KYNA receptor
• Kynurenic acid receptor

Background

GPR35 is an Orphan-A GPCR with an unknown ligand. GPR35 expression was reported only in rat intestine by Northern hybridization, however, another study showed that GPR35 is expressed widely, including pancreatic islets and skeletal muscle, with higher levels in adult lung, small intestine, colon, and stomach. ESTs have been isolated from human fetal lung/testis/B-cell, colon (normal, cancer, and Crohn's disease), breast cancer, pancreatic cancer, and leukemia libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR35 Antibody (NBP2-24640)(8)

Publications using NBP2-24640

• Li T, Yue Y, Ma Y et al. Fasting-mimicking diet alleviates inflammatory pain by inhibiting neutrophil extracellular traps formation and neuroinflammation in the spinal cord Cell communication and signaling : CCS 2023-09-21 [PMID: 37735678] (WB, Mouse)
• Kim C, Kim Y, Lim JY et al. Pamoic Acid-Induced Peripheral GPR35 Activation Improves Pruritus and Dermatitis British journal of pharmacology 2023-07-28 [PMID: 37501600]
• Ma J, Chen P, Deng B, Wang R Kynurenic acid promotes osteogenesis via the Wnt/?-catenin signaling In vitro cellular & developmental biology. Animal 2023-06-08 [PMID: 37291335]
• Zhang Y, Shi T, He Y GPR35 regulates osteogenesis via the Wnt/GSK3 beta/beta-catenin signaling pathway Biochemical and biophysical research communications 2021-06-04 [PMID: 33839412]
• Kaya B, Donas C, Wuggenig P et al. Lysophosphatidic Acid-Mediated GPR35 Signaling in CX3CR1+ Macrophages Regulates Intestinal Homeostasis Cell Rep 2020-08-04 [PMID: 32755573] (ICC/IF, Human)
• Nam SY, Park SJ, Im DS. Protective effect of lodoxamide on hepatic steatosis through GPR35. Cell. Signal. 2018-10-07 [PMID: 30304698] (WB, Human)
• Farooq S, Hou Y, Li Hainan et al. Disruption of GPR35 Exacerbates Dextran Sulfate Sodium-Induced Colitis in Mice Dig Dis Sci 2018-07-24 [PMID: 30043283] (WB, IHC-P, Mouse, Human)
• Park SJ, Lee SJ, Nam SY, Im DS. GPR35 mediates lodoxamide-induced migration inhibitory response but not CXCL17-induced migration stimulatory response in THP-1 cells; Is GPR35 a receptor for CXCL17 Br. J. Pharmacol. 2017-10-25 [PMID: 29068046] (Human)

Bioinformatics

• Gene Symbol: GPR35