RASAL1 Antibodies
We offer RASAL1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each RASAL1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RASAL1 Antibodies can be used in a variety of model species: Human. Use the list below to choose the RASAL1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RASAL1 polyclonal antibodies.
Alternate Names for RASAL1 Antibodies
anti-RASAL1 antibody, anti-RAS protein activator like 1 (GAP1 like) antibody, anti-RASALGAP1 like protein antibody, anti-rasGAP-activating-like protein 1 antibody
6 Results for "rasal1" in Primary Antibodies
![Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 inhibits the ERK1/2 pathway, but not p38 pathway. Expression of Rasal1 in T cells inhibits ERK1,2 phosphorylation. Upper and lower panels: two independent experiments where primary mouse T cells were transfected with Flag-tagged Rasal1 and were ligated with anti-CD3 for 5 min followed by blotting with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204); anti-ERK1/2, anti-ZAP-70 or anti-Flag. Flag-PLK expression served as a negative control (top panel) (n > 2). Upper right panel shows endogenous levels of Rasal1 in activated primary T cells. Lower right panel: histogram showing the inhibition of pERK relative to ERK expression. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-12544-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Western-Blot-NBP1-32776-img0006.jpg "Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 inhibits the ERK1/2 pathway, but not p38 pathway. Expression of Rasal1 in T cells inhibits ERK1,2 phosphorylation. Upper and lower panels: two independent experiments where primary mouse T cells were transfected with Flag-tagged Rasal1 and were ligated with anti-CD3 for 5 min followed by blotting with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204); anti-ERK1/2, anti-ZAP-70 or anti-Flag. Flag-PLK expression served as a negative control (top panel) (n > 2). Upper right panel shows endogenous levels of Rasal1 in activated primary T cells. Lower right panel: histogram showing the inhibition of pERK relative to ERK expression. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-12544-4) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: RASAL1 Antibody [NBP1-32776] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32776-img0003.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Western-Blot-NBP1-32776-img0004.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - Protein expression was upregulated in older female crab-eating monkey brain. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Western-Blot-NBP1-32776-img0005.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 inhibits in vitro anti-CD3 & dendritic cell-peptide induced T-cell activation. a Antigen (OVA323-39)-dependent activation & proliferation of DO11.10 CD4 + cells expressing Flag-tagged Rasal1 compared with control cells at different OVA peptide concentrations (5–10 ng). CTLA-Ig was used as a control. Upper inset shows expression of Flag-tagged Rasal using anti-Flag in blotting (n = 3). b Inhibition of T-cell responses by Rasal1 to increasing anti-CD3 concentrations. Upper inset: effects of Rasal1 at 48 & 72 h post-anti-CD3 activation (n = 3). c Anti-CD3-driven NFATc1/AP1 promoter activity in Jurkat T cells was inhibited by the presence of Flag-tagged Rasal1 (n = 3). d siRNA (esiRNA) knockdown of Rasal1 enhances T-cell proliferation. esiRNA were tested for their knockdown efficiency by western blot (top right panel), esiRNA1 & 2 correspond to 500 nM & 1000 nM concentrations (n = 3). e Contact time between D011.10 CD4+T-cells expressing Flag-Rasal1 (red) or empty vector (blue) & OVA peptide on bone marrow dendritic cells (BMDCs). Dot blot of T cell/DC contact duration time in presence of OVA was unaffected by Flag-tagged Rasal1 or vector transfection (n = 3). Representative plots show mean with ± SD. p-value was calculated by unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32776_rabbit-polyclonal-rasal1-antibody-310202416171418.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - TCR complex purification & Rasal1 association with ZAP-70 in the TCR complex. a Analytic gel from tandem affinity purification (TAP) of TCR plus associated proteins captured from TCR-constituted cells (TCR complex) versus TCR-null negative control cells (control) (left panel). Chart showing the list of TCR-associated proteins identified by MS analysis (right upper panel). The structure of Rasal1 (right lower panel). b Co-precipitation of ZAP-70 with TCR from 3A9 T cells. 3A9 cells were stimulated for 10 min with anti-CD3 followed by immunoprecipitation with anti-TCR zeta & blotting with anti-ZAP-70 or anti-zeta. Lane 1: resting T cells; lane 2: anti-CD3-stimulated T cells. c Rasal1 expression is induced with anti-CD3 activation. Relative fold change expression of Rasal1 versus Rasal3 upon anti-CD3 stimulation of CD4+ & CD8 + peripheral T cells. 18 s rRNA served as a control. d Anti-CD3 induces Rasal1 translocation to the membranes of T cells. Stimulation-dependent association of Rasal1 with the membrane (upper panel) versus cytosolic Rasal1 (lower panel). Anti-actin served as a loading control. LAT was used as a marker of membrane enrichment fractionation (n = 3) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32776_rabbit-polyclonal-rasal1-antibody-310202416175286.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 binds to the immune cell tyrosine kinase ZAP-70. a Proximity ligation assay (PLA) of Rasal1 & ZAP-70 in Jurkat T-cells stained with DAPI, anti-Rasal1, & anti-ZAP-70. Positive PLA signal (fluorescent dots) indicates a distance of 30–40 nm between Rasal1 & ZAP-70 in anti-CD3-activated T cells, indicating their close proximity (n = 2). b Anti-Rasal1 co-precipitates ZAP-70 from primary mouse T cells. Lane 1–2 loading controls, lanes 3-4: anti-Rasal1 IP: lanes 2 & 4. Cells were stimulated with anti-CD3 ligation for 5 min vs IgG control ligation in lanes 1 & 3. c Anti-Rasal1 co-precipitates ZAP-70 from transfected Jurkat T cells. Lanes 1: nonligated (i.e., IgG); lane 2: anti-CD3 ligated T cells for 5 min. Lower panel shows similar level of ZAP-70 expression in lysates of (n = 2) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32776_rabbit-polyclonal-rasal1-antibody-31020241618945.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 binds to the immune cell tyrosine kinase ZAP-70. a Proximity ligation assay (PLA) of Rasal1 & ZAP-70 in Jurkat T-cells stained with DAPI, anti-Rasal1, & anti-ZAP-70. Positive PLA signal (fluorescent dots) indicates a distance of 30–40 nm between Rasal1 & ZAP-70 in anti-CD3-activated T cells, indicating their close proximity (n = 2). b Anti-Rasal1 co-precipitates ZAP-70 from primary mouse T cells. Lane 1–2 loading controls, lanes 3-4: anti-Rasal1 IP: lanes 2 & 4. Cells were stimulated with anti-CD3 ligation for 5 min vs IgG control ligation in lanes 1 & 3. c Anti-Rasal1 co-precipitates ZAP-70 from transfected Jurkat T cells. Lanes 1: nonligated (i.e., IgG); lane 2: anti-CD3 ligated T cells for 5 min. Lower panel shows similar level of ZAP-70 expression in lysates of (n = 2) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32776_rabbit-polyclonal-rasal1-antibody-310202416163728.jpg)
![Immunocytochemistry/Immunofluorescence: RASAL1 Antibody [NBP2-13204] - Staining of human cell line U-2 OS shows positivity in plasma membrane and cytoplasm.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13204-img0004.jpg "Immunocytochemistry/Immunofluorescence: RASAL1 Antibody [NBP2-13204] - Staining of human cell line U-2 OS shows positivity in plasma membrane and cytoplasm.")
![Immunohistochemistry-Paraffin: RASAL1 Antibody [NBP2-13204] - Staining of human adrenal gland shows strong membranous with additional cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Immunohistochemistry-Paraffin-NBP2-13204-img0001.jpg)
![Western Blot: RASAL1 Antibody [NB100-55388] - (0.3ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Western-Blot-NB100-55388-img0003.jpg "Western Blot: RASAL1 Antibody [NB100-55388] - (0.3ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: RASAL1 Antibody [H00008437-B01P] - Analysis of RASAL1 expression in rat brain.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Western-Blot-H00008437-B01P-img0001.jpg "Western Blot: RASAL1 Antibody [H00008437-B01P] - Analysis of RASAL1 expression in rat brain.")
![Western Blot: RASAL1 Antibody [H00008437-B01P] - Analysis of RASAL1 expression in transfected 293T cell line by RASAL1 polyclonal antibody. Lane 1: RASAL1 transfected lysate(85.36 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Western-Blot-H00008437-B01P-img0002.jpg)
![Western Blot: RASAL1 Antibody [NBP2-94543] - Western blot analysis of extracts of various cell lines, using RASAL1 antibody (NBP2-94543) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/RASAL1 Antibody-Western Blot-NBP2-94543-img0001.jpg "Western Blot: RASAL1 Antibody [NBP2-94543] - Western blot analysis of extracts of various cell lines, using RASAL1 antibody (NBP2-94543) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunoprecipitation:RASAL1 AntibodyNBP3-29136] -Detection of human RASAL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RPMI-8226 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RASAL1 antibody (NBP3-29136 lot 1) used for IP at 1 µg per reaction. RASAL1 was also immunoprecipitated by rabbit anti-RASAL1 antibody BL24199. For blotting immunoprecipitated RASAL1, NBP3-29136 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29136_rabbit-rasal1-pab-301220241633618.jpg "Immunoprecipitation:RASAL1 AntibodyNBP3-29136] -Detection of human RASAL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RPMI-8226 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RASAL1 antibody (NBP3-29136 lot 1) used for IP at 1 µg per reaction. RASAL1 was also immunoprecipitated by rabbit anti-RASAL1 antibody BL24199. For blotting immunoprecipitated RASAL1, NBP3-29136 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")