Neurofibromin 1 Antibody

Images

 
Western Blot: Neurofibromin 1 Antibody [NB300-155] - Western Blot of (A, B, C above).
Immunohistochemistry: Neurofibromin 1 Antibody [NB300-155] - NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Neurofibromin 1 Antibody Summary

Immunogen
An N-terminal peptide of Neurofibromin 1 (within amino acids 1-50). [Uniprot: P21359]
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NF1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Frozen 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
Application Notes
In Western blot, a band-set at 220-240 kDa and a higher MW band at 450 kDa (uncharacterized) can be seen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

For IHC, antigen retrieval is not required for immunostaining of frozen sections. Antigen retrieval with trypsin or citrate improves immunostaining of paraffin sections.

Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23910086)
Control
Neurofibromin 1 Knockout HeLa Cell Lysate
Control Peptide
Neurofibromin 1 Peptide (NB300-155PEP)
Publications
Read Publications using
NB300-155 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Neurofibromin 1 Antibody

  • DKFZp686J1293
  • FLJ21220
  • Neurofibromatosis-related protein NF-1
  • neurofibromin 1
  • neurofibromin
  • NFNS
  • VRNF
  • WSS

Background

Neurofibromin 1 (NF1), the Nf1 gene product, is a large protein which contains a central Ras-GTPase-activating (RasGAP) domain and is suggested to function as a negative regulator of proto-oncogene RAS and downstream effectors. It stimulates the GTPase activity of RAS and acts as regulator of RAS signaling. Consistent with its biochemical activity, NF1 functions as a tumor-suppressor gene in juvenile myelomonocytic leukemia (JMML), and diseased BM cells exhibit aberrant Raf/MEK/ERK signaling. Moreover, NF1 shows greater affinity for Ras GAP, but lower specific activity. Besides JMML, defects in NF1 have also been linked to neurofibromatosis type 1 (NF1), Watson syndrome (WS), familial spinal neurofibromatosis (FSNF), neurofibromatosis-Noonan syndrome (NFNS), colorectal cancer (CRC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neurofibromin 1 Antibody (NB300-155)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Neurofibromin 1 Antibody (NB300-155) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Blogs on Neurofibromin 1.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.

Neurofibromatosis Infographic
Neurofibromatosis (NF) is a genetic disorder caused by mutations in the NF1, NF2 or SMARCB1 genes which lead to tumor growth on nerves throughout the body. Although the tumors are usually benign, they still require chemotherapy to shrink and may becom...  Read full blog post.

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Bioinformatics

Gene Symbol NF1
Entrez
Uniprot