RanGAP1 Antibody - BSA Free

Western Blot: RanGAP1 Antibody [NBP1-18915] - Detection of human and mouse RanGAP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody NBP1-18915 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: RanGAP1 Antibody [NBP1-18915] - Detection of human RanGAP1 by immunohistochemistry. Samples: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RanGAP1 Cat. No. NBP1-18915 (left) and another rabbit anti-RanGAP1 antibody (right) used at a dilution 1ug/ml. Detection: DAB

Western Blot: RanGAP1 Antibody [NBP1-18915] - Whole cell lysate from 293T and HeLa cells. RanGAP1 was also immunoprecipitated by rabbit anti-RanGAP1 antibody NBP1-18916.

Immunoprecipitation: RanGAP1 Antibody [NBP1-18915] - Detection of human RanGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody NBP1-18915 used for IP at 6 ug per reaction. RanGAP1 was also immunoprecipitated by another rabbit anti-RanGAP1 antibody. For blotting immunoprecipitated RanGAP1, NBP1-18915 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RanGAP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RanGAP1 Antibody - BSA Free (NBP1-18915) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human Ran GTPase activating protein 1 using the numbering given in entry NP_002874.1 (GeneID 5905).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RANGAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 63.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RanGAP1 Antibody - BSA Free

• Fug1
• KIAA1835Fug1
• MGC20266
• Ran GTPase activating protein 1
• ran GTPase-activating protein 1
• RanGAP1
• SD
• segregation distorter homolog (Drosophila)
• segregation distorter homolog
• segregation distortion

Background

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RANGAP1
• Entrez:
  • 5905(Human)
• Uniprot:
  • P46060(Human)