| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73797 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33591-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7834-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56215-25ul | |||
![Western Blot: RanGAP1 Antibody (1B4) [NBP2-02623] Analysis of extracts (35ug) from 9 different cell lines by using anti-RanGAP1 monoclonal antibody.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Western-Blot-NBP2-02623-img0009.jpg "Western Blot: RanGAP1 Antibody (1B4) [NBP2-02623] Analysis of extracts (35ug) from 9 different cell lines by using anti-RanGAP1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RanGAP1.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Immunocytochemistry-Immunofluorescence-NBP2-02623-img0008.jpg)
![Immunohistochemistry-Paraffin: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of paraffin-embedded Human prostate tissue using anti-RanGAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Immunohistochemistry-Paraffin-NBP2-02623-img0007.jpg)
![Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RanGAP1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Flow-Cytometry-NBP2-02623-img0003.jpg)
![Western Blot: RanGAP1 Antibody (1B4) [NBP2-02623] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RanGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RanGAP1.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Western-Blot-NBP2-02623-img0004.jpg)
![Immunohistochemistry-Paraffin: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RanGAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Immunohistochemistry-Paraffin-NBP2-02623-img0005.jpg)
![Immunohistochemistry-Paraffin: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of paraffin-embedded Human liver tissue using anti-RanGAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Immunohistochemistry-Paraffin-NBP2-02623-img0006.jpg)
![Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - Analysis of Hela cells, using anti-RanGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Flow-Cytometry-NBP2-02623-img0001.jpg)
![Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - Analysis of Jurkat cells, using anti-RanGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Flow-Cytometry-NBP2-02623-img0002.jpg)
![Western Blot: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Western blot analysis of extracts of various cell lines, using RanGAP1 Rabbit mAb (NBP3-16697) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1E7R7-Western-Blot-NBP3-16697-img0007.jpg "Western Blot: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Western blot analysis of extracts of various cell lines, using RanGAP1 Rabbit mAb (NBP3-16697) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunofluorescence analysis of U-2 OS cells using RanGAP1 Rabbit mAb (NBP3-16697) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1E7R7-Immunocytochemistry-Immunofluorescence-NBP3-16697-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunofluorescence analysis of C6 cells using RanGAP1 Rabbit mAb (NBP3-16697) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1E7R7-Immunocytochemistry-Immunofluorescence-NBP3-16697-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunofluorescence analysis of NIH-3T3 cells using RanGAP1 Rabbit mAb (NBP3-16697) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1E7R7-Immunocytochemistry-Immunofluorescence-NBP3-16697-img0005.jpg)
![Immunohistochemistry: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunohistochemistry analysis of RanGAP1 in paraffin-embedded rat testis tissue using RanGAP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16697_rabbit-rangap1-mab-1e7r7-8220252010412.jpg)
![Immunohistochemistry: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunohistochemistry analysis of RanGAP1 in paraffin-embedded mouse kidney tissue using RanGAP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16697_rabbit-rangap1-mab-1e7r7-822025209862.jpg)
![Immunohistochemistry: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunohistochemistry analysis of RanGAP1 in paraffin-embedded rat liver tissue using RanGAP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16697_rabbit-rangap1-mab-1e7r7-822025208443.jpg)
![Immunocytochemistry/ Immunofluorescence: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunofluorescence analysis of NIH-3T3 cells using RanGAP1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16697_rabbit-rangap1-mab-1e7r7-822025209848.jpg)
![Immunohistochemistry: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunohistochemistry analysis of RanGAP1 in paraffin-embedded human colon carcinoma tissue using RanGAP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16697_rabbit-rangap1-mab-1e7r7-8220252010442.jpg)
![Immunohistochemistry: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunohistochemistry analysis of RanGAP1 in paraffin-embedded mouse brain tissue using RanGAP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16697_rabbit-rangap1-mab-1e7r7-822025208410.jpg)
![Western Blot: RanGAP1 Antibody [NBP2-33591] - Analysis using Anti-RANGAP1 antibody NBP2-33591 (A) shows similar pattern to independent antibody NBP2-56215 (B).](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP2-33591-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RanGAP1 Antibody [NBP2-33591] - Analysis using Anti-RANGAP1 antibody NBP2-33591 (A) shows similar pattern to independent antibody NBP2-56215 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody [NBP2-33591] - Staining of human cell line A-431 shows localization to nuclear membrane, cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33591-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RanGAP1 Antibody [NBP2-33591] - Staining in human testis and liver tissues using anti-RANGAP1 antibody. Corresponding RANGAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-33591-img0008.jpg)
![Immunohistochemistry-Paraffin: RanGAP1 Antibody [NBP2-33591] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-33591-img0006.jpg)
![Immunohistochemistry-Paraffin: RanGAP1 Antibody [NBP2-33591] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-33591-img0007.jpg)
![Western Blot: RanGAP1 Antibody [NB100-1384] - (0.2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NB100-1384-img0005.jpg "Western Blot: RanGAP1 Antibody [NB100-1384] - (0.2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: RanGAP1 Antibody [NB100-1384] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunohistochemistry-Paraffin-NB100-1384-img0004.jpg)
![Western Blot: RanGAP1 Antibody [NBP1-18915] - Detection of human and mouse RanGAP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody NBP1-18915 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP1-18915-img0005.jpg "Western Blot: RanGAP1 Antibody [NBP1-18915] - Detection of human and mouse RanGAP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody NBP1-18915 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: RanGAP1 Antibody [NBP1-18915] - Detection of human RanGAP1 by immunohistochemistry. Samples: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RanGAP1 Cat. No. NBP1-18915 (left) and another rabbit anti-RanGAP1 antibody (right) used at a dilution 1ug/ml. Detection: DAB](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunohistochemistry-NBP1-18915-img0003.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: RanGAP1 Antibody [NBP1-18915] - Whole cell lysate from 293T and HeLa cells. RanGAP1 was also immunoprecipitated by rabbit anti-RanGAP1 antibody NBP1-18916.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP1-18915-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RanGAP1 Antibody [NBP1-18915] - Detection of human RanGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody NBP1-18915 used for IP at 6 ug per reaction. RanGAP1 was also immunoprecipitated by another rabbit anti-RanGAP1 antibody. For blotting immunoprecipitated RanGAP1, NBP1-18915 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunoprecipitation-NBP1-18915-img0004.jpg)
![Western Blot: RanGAP1 Antibody [NBP2-20068] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody diluted at 1:2000. The observed M.W. is based on the publication: PMID: 18706886](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP2-20068-img0003.jpg "Western Blot: RanGAP1 Antibody [NBP2-20068] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody diluted at 1:2000. The observed M.W. is based on the publication: PMID: 18706886")
![Western Blot: RanGAP1 Antibody [NBP2-20068] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody diluted at 1:2000. The observed M.W. is based on the publication: PMID: 18706886](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP2-20068-img0002.jpg)
![Western Blot: RanGAP1 Antibody [NBP2-20068] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody [N1N3] (NBP2-20068) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-20068_rabbit-polyclonal-rangap1-antibody-91020242001051.jpg)
![Western Blot: RanGAP1 Antibody [NBP2-56215] - Analysis using Anti-RANGAP1 antibody NBP2-56215 (A) shows similar pattern to independent antibody NBP2-33591 (B).](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP2-56215-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RanGAP1 Antibody [NBP2-56215] - Analysis using Anti-RANGAP1 antibody NBP2-56215 (A) shows similar pattern to independent antibody NBP2-33591 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody [NBP2-56215] - Staining of human cell line U-2 OS shows localization to nuclear membrane, cytosol & vesicles.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56215-img0001.jpg)
![Western Blot: RanGAP1 Antibody [NBP2-20067] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP2-20067-img0001.jpg "Western Blot: RanGAP1 Antibody [NBP2-20067] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RanGAP1 Antibody [NBP2-20067] - Immunohistochemical analysis of paraffin-embedded Trachea, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-20067-img0002.jpg)
![Western Blot: RanGAP1 Overexpression Lysate (Adult Normal) [NBL1-15144] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RanGAP1.](http://images.novusbio.com/fullsize/RanGAP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15144-img0002.jpg "Western Blot: RanGAP1 Overexpression Lysate (Adult Normal) [NBL1-15144] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RanGAP1.")