RanGAP1 Antibodies
We offer RanGAP1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot. Each RanGAP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RanGAP1 Antibodies can be used in a variety of model species: Canine, Human, Mouse, Rat. Use the list below to choose the RanGAP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RanGAP1 polyclonal antibodies and browse our RanGAP1 monoclonal antibody catalog.
Alternate Names for RanGAP1 Antibodies
anti-RanGAP1 antibody, anti-KIAA1835Fug1 antibody, anti-MGC20266 antibody, anti-Ran GTPase activating protein 1 antibody, anti-ran GTPase-activating protein 1 antibody, anti-SD antibody, anti-segregation distorter homolog (Drosophila) antibody, anti-segregation distorter homolog antibody, anti-segregation distortion antibody
9 Results for "rangap1" in Primary Antibodies
![Western Blot: RanGAP1 Antibody (1B4) [NBP2-02623] Analysis of extracts (35ug) from 9 different cell lines by using anti-RanGAP1 monoclonal antibody.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Western-Blot-NBP2-02623-img0009.jpg "Western Blot: RanGAP1 Antibody (1B4) [NBP2-02623] Analysis of extracts (35ug) from 9 different cell lines by using anti-RanGAP1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RanGAP1.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Immunocytochemistry-Immunofluorescence-NBP2-02623-img0008.jpg)
![Immunohistochemistry-Paraffin: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of paraffin-embedded Human prostate tissue using anti-RanGAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Immunohistochemistry-Paraffin-NBP2-02623-img0007.jpg)
![Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RanGAP1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Flow-Cytometry-NBP2-02623-img0003.jpg)
![Western Blot: RanGAP1 Antibody (1B4) [NBP2-02623] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RanGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RanGAP1.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Western-Blot-NBP2-02623-img0004.jpg)
![Immunohistochemistry-Paraffin: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RanGAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Immunohistochemistry-Paraffin-NBP2-02623-img0005.jpg)
![Immunohistochemistry-Paraffin: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of paraffin-embedded Human liver tissue using anti-RanGAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Immunohistochemistry-Paraffin-NBP2-02623-img0006.jpg)
![Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - Analysis of Hela cells, using anti-RanGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Flow-Cytometry-NBP2-02623-img0001.jpg)
![Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - Analysis of Jurkat cells, using anti-RanGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1B4-Flow-Cytometry-NBP2-02623-img0002.jpg)
![Western Blot: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Western blot analysis of extracts of various cell lines, using RanGAP1 Rabbit mAb (NBP3-16697) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1E7R7-Western-Blot-NBP3-16697-img0007.jpg "Western Blot: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Western blot analysis of extracts of various cell lines, using RanGAP1 Rabbit mAb (NBP3-16697) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunofluorescence analysis of U-2 OS cells using RanGAP1 Rabbit mAb (NBP3-16697) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1E7R7-Immunocytochemistry-Immunofluorescence-NBP3-16697-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunofluorescence analysis of C6 cells using RanGAP1 Rabbit mAb (NBP3-16697) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1E7R7-Immunocytochemistry-Immunofluorescence-NBP3-16697-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunofluorescence analysis of NIH-3T3 cells using RanGAP1 Rabbit mAb (NBP3-16697) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-1E7R7-Immunocytochemistry-Immunofluorescence-NBP3-16697-img0005.jpg)
![Immunohistochemistry: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunohistochemistry analysis of RanGAP1 in paraffin-embedded rat testis tissue using RanGAP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16697_rabbit-rangap1-mab-1e7r7-8220252010412.jpg)
![Immunohistochemistry: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunohistochemistry analysis of RanGAP1 in paraffin-embedded mouse kidney tissue using RanGAP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16697_rabbit-rangap1-mab-1e7r7-822025209862.jpg)
![Immunohistochemistry: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunohistochemistry analysis of RanGAP1 in paraffin-embedded rat liver tissue using RanGAP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16697_rabbit-rangap1-mab-1e7r7-822025208443.jpg)
![Immunocytochemistry/ Immunofluorescence: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunofluorescence analysis of NIH-3T3 cells using RanGAP1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16697_rabbit-rangap1-mab-1e7r7-822025209848.jpg)
![Immunohistochemistry: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunohistochemistry analysis of RanGAP1 in paraffin-embedded human colon carcinoma tissue using RanGAP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16697_rabbit-rangap1-mab-1e7r7-8220252010442.jpg)
![Immunohistochemistry: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunohistochemistry analysis of RanGAP1 in paraffin-embedded mouse brain tissue using RanGAP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16697_rabbit-rangap1-mab-1e7r7-822025208410.jpg)
![Western Blot: RanGAP1 Antibody [NBP2-33591] - Analysis using Anti-RANGAP1 antibody NBP2-33591 (A) shows similar pattern to independent antibody NBP2-56215 (B).](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP2-33591-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RanGAP1 Antibody [NBP2-33591] - Analysis using Anti-RANGAP1 antibody NBP2-33591 (A) shows similar pattern to independent antibody NBP2-56215 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody [NBP2-33591] - Staining of human cell line A-431 shows localization to nuclear membrane, cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33591-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RanGAP1 Antibody [NBP2-33591] - Staining in human testis and liver tissues using anti-RANGAP1 antibody. Corresponding RANGAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-33591-img0008.jpg)
![Immunohistochemistry-Paraffin: RanGAP1 Antibody [NBP2-33591] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-33591-img0006.jpg)
![Immunohistochemistry-Paraffin: RanGAP1 Antibody [NBP2-33591] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-33591-img0007.jpg)
![Western Blot: RanGAP1 Antibody [NB100-1384] - (0.2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NB100-1384-img0005.jpg "Western Blot: RanGAP1 Antibody [NB100-1384] - (0.2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: RanGAP1 Antibody [NB100-1384] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunohistochemistry-Paraffin-NB100-1384-img0004.jpg)
![Western Blot: RanGAP1 Antibody [NBP1-18915] - Detection of human and mouse RanGAP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody NBP1-18915 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP1-18915-img0005.jpg "Western Blot: RanGAP1 Antibody [NBP1-18915] - Detection of human and mouse RanGAP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody NBP1-18915 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: RanGAP1 Antibody [NBP1-18915] - Detection of human RanGAP1 by immunohistochemistry. Samples: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RanGAP1 Cat. No. NBP1-18915 (left) and another rabbit anti-RanGAP1 antibody (right) used at a dilution 1ug/ml. Detection: DAB](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunohistochemistry-NBP1-18915-img0003.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: RanGAP1 Antibody [NBP1-18915] - Whole cell lysate from 293T and HeLa cells. RanGAP1 was also immunoprecipitated by rabbit anti-RanGAP1 antibody NBP1-18916.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP1-18915-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RanGAP1 Antibody [NBP1-18915] - Detection of human RanGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody NBP1-18915 used for IP at 6 ug per reaction. RanGAP1 was also immunoprecipitated by another rabbit anti-RanGAP1 antibody. For blotting immunoprecipitated RanGAP1, NBP1-18915 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunoprecipitation-NBP1-18915-img0004.jpg)
![Western Blot: RanGAP1 Antibody [NBP2-20068] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody diluted at 1:2000. The observed M.W. is based on the publication: PMID: 18706886](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP2-20068-img0003.jpg "Western Blot: RanGAP1 Antibody [NBP2-20068] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody diluted at 1:2000. The observed M.W. is based on the publication: PMID: 18706886")
![Western Blot: RanGAP1 Antibody [NBP2-20068] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody diluted at 1:2000. The observed M.W. is based on the publication: PMID: 18706886](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP2-20068-img0002.jpg)
![Western Blot: RanGAP1 Antibody [NBP2-20068] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody [N1N3] (NBP2-20068) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-20068_rabbit-polyclonal-rangap1-antibody-91020242001051.jpg)
![Western Blot: RanGAP1 Antibody [NBP2-56215] - Analysis using Anti-RANGAP1 antibody NBP2-56215 (A) shows similar pattern to independent antibody NBP2-33591 (B).](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP2-56215-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RanGAP1 Antibody [NBP2-56215] - Analysis using Anti-RANGAP1 antibody NBP2-56215 (A) shows similar pattern to independent antibody NBP2-33591 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody [NBP2-56215] - Staining of human cell line U-2 OS shows localization to nuclear membrane, cytosol & vesicles.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56215-img0001.jpg)
![Western Blot: RanGAP1 Antibody [NBP2-20067] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Western-Blot-NBP2-20067-img0001.jpg "Western Blot: RanGAP1 Antibody [NBP2-20067] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RanGAP1 Antibody [NBP2-20067] - Immunohistochemical analysis of paraffin-embedded Trachea, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/RanGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-20067-img0002.jpg)