RanBP3 Antibody

Western Blot: RanBP3 Antibody [NB100-74647] - Detection of Human and Mouse RanBP3 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RanBP3 antibody NB100-74647 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RanBP3 was also immunoprecipitated by another rabbit anti-RanBP3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Immunohistochemistry: RanBP3 Antibody [NB100-74647] - Sample: FFPE sections of human ovarian carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti- RanBP3 used at a dilution of 1:200 (1ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy a

• Reactivity: Hu, Mu, Pm
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RanBP3 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human RAN binding protein 3 using the numbering given in entry NP_015561.1 (GeneID 8498).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RANBP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RanBP3 Antibody

• DKFZp586I1520
• RAN binding protein 3
• ran-binding protein 3
• RAN-binding protein-3
• RanBP3

Background

This gene encodes a protein with a RanBD1 domain that is found in both the nucleus and cytoplasm. This protein plays a role in nuclear export as part of a heteromeric complex. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RanBP3 Antibody (NB100-74647)(3)

Publications using NB100-74647

• Ogawa Y, Imamoto N Methods to separate nuclear soluble fractions reflecting localizations in living cells iScience 2021-11-01 [PMID: 34934922] (ICC/IF, WB, Human)
• Fang W, Deng Z, Benadjaoud F et al. Cathepsin B deficiency ameliorates liver lipid deposition, inflammatory cell infiltration, and fibrosis after diet-induced nonalcoholic steatohepatitis Transl Res 2020-05-11 [PMID: 32434697] (WB, Mouse)
• Oka M, Asally M, Yasuda Y et al. The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export. Mol Biol Cell 2010-06-01 [PMID: 20375145] (WB, Mouse)

Bioinformatics

• Gene Symbol: RANBP3
• Entrez:
  • 8498(Human)
  • 71810(Mouse)
• Uniprot:
  • Q9H6Z4(Human)