SOS2 Antibody [Unconjugated]

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and 293T human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human SOS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOS2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SOS2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SOS2
  Met1-Ser384
  Accession # Q07890
• Specificity: Detects human SOS2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SOS2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOS2 Antibody [Unconjugated]

• FLJ25596
• guanine nucleotide exchange factor
• guanine nucleotide releasing factor
• son of sevenless (Drosophilia) homolog 2
• son of sevenless homolog 2 (Drosophila)
• son of sevenless homolog 2
• SOS2
• SOS-2

Background

SOS2 (Son of sevenless homolog 2) is a 160-170 kDa protein that regulates Ras signaling. It is widely expressed, and serves as a mediator of guanine nucleotide phosphate exchange. In the resting cell, cytoplasmic SOS2 forms a heterodimer with Grb2, and a heterotrimer with Eps8 and E3b1. Upon RTK activation, the SOS heterodimer is recruited to the cell membrane, where it contacts GDP-bound Ras. This allows it to facilitate a GTP-for-GDP exchange that activates Ras. The heterotrimer interacts with Rac on actin filaments. SOS2 activity parallels that of SOS1. Relative to SOS1, however, SOS2 binds Grb2 with higher affinity and shows less biological activity due to a shorter half-life. Human SOS2 is 1332 amino acids (aa) in length and contains one histone fold (aa 97-169), PH (aa 439-546) and REM (aa 595-739) domains that interact with Ras, and a Pro-rich region that binds to Grb2 (aa 1126-1242).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOS2 Antibody (AF6260)(1)

Publication using AF6260

• K McGrail, P Granado-Ma, R Esteve-Pui, S García-Ort, Y Ding, S Sánchez-Re, B Ferrer, J Hernandez-, F Canals, A Manzano, A Navarro-Sa, R Bartrons, O Yanes, M Pérez-Alea, E Muñoz-Cous, V Garcia-Pat, JA Recio BRAF activation by metabolic stress promotes glycolysis sensitizing NRASQ61-mutated melanomas to targeted therapy Nature Communications, 2022-11-19;13(1):7113. 2022-11-19 [PMID: 36402789] (ICC, Human)

Bioinformatics

• Gene Symbol: SOS2
• Uniprot:
  • Q07890(Human)