PP2C alpha/PPM1A Antibody

Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPM1A antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: PP2C alpha/PPM1A Antibody [NBP1-32751] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PPM1A protein stained by PPM1A antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: PP2C alpha/PPM1A Antibody [NBP1-32751] - Human colon carcinoma, using PPM1A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000

Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PP2C alpha/PPM1A Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PPM1A. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPM1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7)
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for PP2C alpha/PPM1A Antibody

• EC 3.1.3.16
• FLJ42306
• MGC9201
• MMPa-2
• MPPa-1
• phosphatase 2C alpha
• PP2C alpha
• Pp2c1
• PP2CA
• PP2Calpha
• PP2C-ALPHA
• PPM1A
• PPPM1A
• protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• protein phosphatase 1A
• protein phosphatase 2C alpha isoform
• Protein phosphatase 2C isoform alpha
• Protein phosphatase IA
• protein phosphatase, Mg2+/Mn2+ dependent, 1A

Background

Protein phosphatase 2C (PP2C) is a family of serine/threonine specific protein phosphatases. PP2C is a 42 kDa monomeric enzyme with broad substrate specificity and its activity is depended upon divalent cations (e.g. magnesium) (1). In mammals, three members of PP2C family are known; PP2C -alpha, -beta, and -gamma. PP2C

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PP2C alpha/PPM1A Antibody (NBP1-32751)(1)

Publication using NBP1-32751

• Kwon OC, Choi B, Lee EJ et al. The intracellular protein phosphatase magnesium-dependent 1A negatively regulates osteoclast commitment Arthritis & Rheumatology (Hoboken, N.J.) 2019-11-24 [PMID: 31762216]

Bioinformatics

• Gene Symbol: PPM1A