| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92315-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92314-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22406-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97450-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97450-100ul | |||
| BSA Free | NBP2-97450-50ul | |||
![Western Blot: RanBP3 Antibody [NB100-74647] - Detection of Human and Mouse RanBP3 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RanBP3 antibody NB100-74647 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RanBP3 was also immunoprecipitated by another rabbit anti-RanBP3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/RanBP3-Antibody-Western-Blot-NB100-74647-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: RanBP3 Antibody [NB100-74647] - Detection of Human and Mouse RanBP3 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RanBP3 antibody NB100-74647 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RanBP3 was also immunoprecipitated by another rabbit anti-RanBP3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: RanBP3 Antibody [NB100-74647] - Sample: FFPE sections of human ovarian carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti- RanBP3 used at a dilution of 1:200 (1ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy a](http://images.novusbio.com/fullsize/RanBP3-Antibody-Immunohistochemistry-NB100-74647-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: RanBP3 Antibody [NBP1-92315] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/RanBP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92315-img0006.jpg "Immunocytochemistry/Immunofluorescence: RanBP3 Antibody [NBP1-92315] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: RanBP3 Antibody [NBP1-92315] - Staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts and strong positivity in Leydig cells.](http://images.novusbio.com/fullsize/RanBP3-Antibody-Immunohistochemistry-Paraffin-NBP1-92315-img0002.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-92315_rabbit-polyclonal-ranbp3-antibody-3052025101822.jpg)
![Immunocytochemistry/Immunofluorescence: RanBP3 Antibody [NBP1-92314] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/RanBP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92314-img0004.jpg "Immunocytochemistry/Immunofluorescence: RanBP3 Antibody [NBP1-92314] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RanBP3 Antibody [NBP1-92314] - Staining in human testis and pancreas tissues using anti-RANBP3 antibody. Corresponding RANBP3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RanBP3-Antibody-Immunohistochemistry-Paraffin-NBP1-92314-img0006.jpg)
![Immunohistochemistry-Paraffin: RanBP3 Antibody [NBP1-92314] - Staining of human testis shows strong nuclear and cytoplasmic positivity.](http://images.novusbio.com/fullsize/RanBP3-Antibody-Immunohistochemistry-Paraffin-NBP1-92314-img0002.jpg)
![Immunohistochemistry-Paraffin: RanBP3 Antibody [NBP1-92314] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/RanBP3-Antibody-Immunohistochemistry-Paraffin-NBP1-92314-img0005.jpg)
![Western Blot: RanBP3 Antibody (SR2138) [NBP3-22406] - Western blot analysis using NBP3-22406 in 293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22406_rabbit-ranbp3-mab-sr2138-57202315584840.jpg "Western Blot: RanBP3 Antibody (SR2138) [NBP3-22406] - Western blot analysis using NBP3-22406 in 293 cell lysate.")
![Western Blot: RanBP3 Antibody [NBP2-97450] - Anti-RanBP3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 60 kDa](http://images.novusbio.com/fullsize/RanBP3-Antibody-Western-Blot-NBP2-97450-img0004.jpg "Western Blot: RanBP3 Antibody [NBP2-97450] - Anti-RanBP3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 60 kDa")
![Immunohistochemistry-Paraffin: RanBP3 Antibody [NBP2-97450] - Immunochemical staining of human RanBP3 in human epididymis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RanBP3-Antibody-Immunohistochemistry-Paraffin-NBP2-97450-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RanBP3 Antibody [NBP2-97450] - Immunofluorescence staining of RanBP3 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97450 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97450_rabbit-polyclonal-ranbp3-antibody-235202521451728.png)
![Western Blot: RanBP3 Antibody (2E10) [H00008498-M01] - Analysis of RANBP3 expression in transfected 293T cell line by RANBP3 monoclonal antibody (M01), clone 2E10.Lane 1: RANBP3 transfected lysate(42.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RanBP3-Antibody-2E10-Western-Blot-H00008498-M01-img0006.jpg "Western Blot: RanBP3 Antibody (2E10) [H00008498-M01] - Analysis of RANBP3 expression in transfected 293T cell line by RANBP3 monoclonal antibody (M01), clone 2E10.Lane 1: RANBP3 transfected lysate(42.4 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RanBP3 Antibody (2E10) [H00008498-M01] - Analysis of monoclonal antibody to RANBP3 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RanBP3-Antibody-2E10-Immunocytochemistry-Immunofluorescence-H00008498-M01-img0004.jpg)
![Sandwich ELISA: RanBP3 Antibody (2E10) [H00008498-M01] - Detection limit for recombinant GST tagged RANBP3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RanBP3-Antibody-2E10-Sandwich-ELISA-H00008498-M01-img0005.jpg)
![Western Blot: RanBP3 Antibody (12E11) [NBP2-42672] - The cell lysates of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RANBP3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/RanBP3-Antibody-12E11-Western-Blot-NBP2-42672-img0001.jpg "Western Blot: RanBP3 Antibody (12E11) [NBP2-42672] - The cell lysates of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RANBP3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Western Blot: RanBP3 Antibody [NBP2-20066] - Sample (30 ug of whole cell lysate) A: JurKat B: Raji 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RanBP3-Antibody-Western-Blot-NBP2-20066-img0001.jpg "Western Blot: RanBP3 Antibody [NBP2-20066] - Sample (30 ug of whole cell lysate) A: JurKat B: Raji 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RanBP3 Antibody [NBP2-20066] - Immunohistochemical analysis of paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/RanBP3-Antibody-Immunohistochemistry-Paraffin-NBP2-20066-img0002.jpg)
![SDS-Page: RanBP3 Recombinant Protein [NBP2-51726] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/RanBP3-Recombinant-Protein-SDS-Page-NBP2-51726-img0001.jpg "SDS-Page: RanBP3 Recombinant Protein [NBP2-51726] - 15% SDS Page (3 ug)")