Rad9 Antibody - BSA Free

Western Blot: Rad9 Antibody [NBP1-87163] - Analysis in control (vector only transfected HEK293T lysate) and RAD9A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: Rad9 Antibody [NBP1-87163] - Staining of human cell line U-251 MG shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: Rad9 Antibody [NBP1-87163] - Staining in human small intestine and liver tissues using anti-RAD9A antibody. Corresponding RAD9A RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Rad9 Antibody [NBP1-87163] - Staining of human stomach shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: Rad9 Antibody [NBP1-87163] - Staining of human liver shows low expression as expected.

Immunohistochemistry-Paraffin: Rad9 Antibody [NBP1-87163] - Staining of human small intestine shows high expression.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Rad9 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Rad9 Antibody - BSA Free (NBP1-87163) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: AIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAD9A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Rad9 Protein (NBP1-87163PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Rad9 Antibody - BSA Free

• cell cycle checkpoint control protein RAD9A
• DNA repair exonuclease rad9 homolog A
• EC 3.1.11.2
• hRAD9
• RAD9 (S. pombe) homolog
• RAD9 homolog A (S. pombe)
• RAD9

Background

Human Rad9 is a homologue of Schizosaccharomyces pombe Rad9 protein and plays a critical role in cell cycle checkpoint control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD9A