Rad9 Antibody


Western Blot: Rad9 Antibody [NB100-193] - Analysis of Rad9 on HeLa whole cell extracts using NB100-193.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Please see the vial label for concentration. If unlisted please contact technical services.

Rad9 Antibody Summary

Fusion protein of full length human Rad9 protein. [UniProt# Q99638]
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.1% Sodium Azide
Protein G purified


  • Western Blot 1:100
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This Rad9 antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. By Western blot a band is seen at approximately 42 kDa, however multiple bands may be seen between ~40-60 kDa, depending on phosphorylation of the protein.
Read Publications using
NB100-193 in the following applications:

Reactivity Notes


Alternate Names for Rad9 Antibody

  • cell cycle checkpoint control protein RAD9A
  • DNA repair exonuclease rad9 homolog A
  • EC
  • hRAD9
  • RAD9 (S. pombe) homolog
  • RAD9 homolog A (S. pombe)
  • RAD9


Rad9 (cell cycle checkpoint control protein RAD9A or hRAD9) is an evolutionarily conserved protein which serves multiple functions for preserving genomic integrity, and besides its role as intra-S/G2 checkpoint it also implicates in homologous recombination repair, base excision repair and mismatch repair pathways. PRKCD mediated Rad9 phosphorylation is essential for the assembly of 9-1-1 cell-cycle checkpoint response complex which is recruited to DNA lesion upon damage by RFC (RAD17-replication factor C) clamp loader complex. Thereafter, Rad9 acts as sliding clamp platform on DNA for multiple proteins involved in LP-BER (long-patch base excision repair). 9-1-1 complex stimulates DNA polymerase beta activity/stability, and is necessary for the recruitment of RHNO1 to sites of DNA-DSBs that occurs during S phase of cell cycle. Moreover, Rad9 exerts 3'->5' double stranded DNA exonuclease activity, and it transactivate p53 target genes such as p21 and has been shown to induce apoptosis through its interaction with Bcl-2/Bcl-xL.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB
Species: Hu
Applications: WB
Species: Hu
Applications: WB, ICC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Mu
Applications: WB, PEP-ELISA
Species: Hu
Applications: WB, Func, ICC/IF, IP
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PLA
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, IHC-FrFl
Species: Hu
Applications: WB, Flow, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Fi, Gt, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Mk
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Ce, Ch
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu
Applications: WB, IP, PLA
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for Rad9 Antibody (NB100-193)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.

Filter By Application
All Applications
Filter By Species
All Species

Reviews for Rad9 Antibody (NB100-193) (0)

There are no reviews for Rad9 Antibody (NB100-193). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rad9 Antibody (NB100-193). (Showing 1 - 1 of 1 FAQs).

  1. Is there any possibility that Novus would make a ChIP grade antibody? Can you tell me how should I test it, just cross-link and precipitated, then check if there are some DNAs that I am interested?
    • This antibody has not been tested in ChIP. If you are interested in testing the antibody we have an Innovators Reward Program where we reward our customers for analyzing our antibodies in species and applications that are previously untested.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Additional Rad9 Antibody Products

Related Products by Gene

Bioinformatics Tool for Rad9 Antibody (NB100-193)

Discover related pathways, diseases and genes to Rad9 Antibody (NB100-193). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Rad9 Antibody (NB100-193)

Discover more about diseases related to Rad9 Antibody (NB100-193).

Pathways for Rad9 Antibody (NB100-193)

View related products by pathway.

PTMs for Rad9 Antibody (NB100-193)

Learn more about PTMs related to Rad9 Antibody (NB100-193).

Research Areas for Rad9 Antibody (NB100-193)

Find related products by research area.

Blogs on Rad9

There are no specific blogs for Rad9, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol RAD9A

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-193 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought