Rad50 Antibody


Western Blot: Rad50 Antibody [NB100-154] - Detection of Rad50 in HeLa nuclear extracts.
Western Blot: Rad50 Antibody [NB100-154] - WB detection of RAD50 protein in 30ug whole cell lysate of 293T cells using Rad50 antibody at 1:2000 dilution (Submitted via Verified Customer Review).
Simple Western: Rad50 Antibody [NB100-154] - Simple Western lane view shows a specific band for Rad50 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Reactivity Hu, Mu, HaSpecies Glossary
Applications WB, Simple Western, IP, ICC/IF (-)
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Rad50 Antibody Summary

C-terminal mouse RAD50 (604 amino acids). [UniProt# P70388]
Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.02% Sodium Azide


  • ICC/IF (Negative)
  • Western Blot 1:2000
  • Simple Western 1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
This RAD50 antibody is useful for Immunoprecipitation and Western blot, where a band can be seen at 153 kDa. IP has been done with 3-4 X 10(6) cells. ICC/IF does not appear to work. Immunocytochemistry/Immunofluorescence was reported in scientific literature.In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
153 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
NB100-154 in the following applications:

Read Publications using
NB100-154 in the following applications:

  • 3 publications
  • IP
    3 publications
  • WB
    11 publications

Reactivity Notes

Human, mouse and hamster.

Alternate Names for Rad50 Antibody

  • DNA repair protein RAD50
  • EC 3.6
  • EC
  • EC 3.6.3
  • EC
  • hRAD50
  • RAD50 (S. cerevisiae) homolog
  • RAD50 homolog (S. cerevisiae)
  • RAD502
  • RAD50-2


RAD50 (DNA repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. RAD50 is required to bind DNA ends for holding them in close proximity where it facilitates searches for short or long regions of sequence homology in the recombining DNA templates, and also stimulate the activity of DNA ligases and/or restrict MRE11A's nuclease activity to prevent nucleolytic degradation. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis. Defects in RAD50 have been linked to Nijmegen breakage syndrome-like disorder (NBSLD).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rad50 Antibody (NB100-154)(29)

We have publications tested in 3 confirmed species: Human, Mouse, Hamster.

We have publications tested in 3 applications: ICC/IF, IP, WB.

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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB100-154 Applications Species
Patel JP, Puck JM, Srinivasan R et al. Nijmegen Breakage Syndrome Detected by Newborn Screening for T Cell Receptor Excision Circles (TRECs) J. Clin. Immunol. 2015 Feb 13 [PMID:25677497] (WB, Human) WB Human
Kondratova A, Watanabe T, Marotta M et al. Replication fork integrity and intra-S phase checkpoint suppress gene amplification Nucleic Acids Res. 2015 Feb 11 [PMID:25672394] (WB, Hamster) WB Hamster
Okamoto K, Bartocci C, Ouzounov I et al. A two-step mechanism for TRF2-mediated chromosome-end protection. Nature 2013 Feb 6 [PMID:23389450] (IP, WB, Human) IP, WB Human
Murad NA, Cullen JK, McKenzie M et al. Mitochondrial dysfunction in a novel form of autosomal recessive ataxia Mitochondrion 2012 Nov 22 [PMID:23178371] (WB, Human) WB Human
Lu CS, Truong LN, Aslanian A et al. The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination J Biol Chem 2012 Oct 31 [PMID:23115235] (WB, Human) WB Human
Buisson R, Masson JY. PALB2 self-interaction controls homologous recombination Nucleic Acids Res 2012 Aug 31 [PMID:22941656] (Human) Human
Thompson R, Montano R, Eastman A. The Mre11 Nuclease Is Critical for the Sensitivity of Cells to Chk1 Inhibition PLoS One 2012 [PMID:22937147] (WB, Human) WB Human
He J, Shi LZ, Truong LN et al. Rad50 Zinc Hook Is Important for the Mre11 Complex to Bind Chromosomal DNA Double-stranded Breaks and Initiate Various DNA Damage Responses J Biol Chem 2012 Sep 14 [PMID:22833675] (WB, Human) WB Human
Karen KA, Hoey PJ, Young CSH et al. Temporal regulation of the Mre11-Rad50-Nbs1 complex during adenovirus infection. J Virol;83(9):4565-73. 2009 May. [PMID:19244322]
Bouska A, Lushnikova T, Plaza S et al. Mdm2 promotes genetic instability and transformation independent of p53. Mol Cell Biol;28(15):4862-74. 2008 Aug. [PMID:18541670]
Show All 29 Publications.

Reviews for Rad50 Antibody (NB100-154) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-154:
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Images Ratings Applications Species Date Details
Western Blot Rad50 NB100-154
reviewed by:
WB Human 12/17/2014


ApplicationWestern Blot
Sample Tested293T lysate


Blocking Details5% BSA in PBS

Primary Anitbody

Dilution Ratio1:2000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker

Secondary Antibody

Secondary Descriptionanti-Rabbit IgG HRP conjugate (Jackson Labs)
Secondary Manufacturer Cat#111-005-045
Secondary Concentration1:20000


Detection NotesProbed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 30 sec exposure

Product General Protocols

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FAQs for Rad50 Antibody (NB100-154) (0)

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Blogs on Rad50.

RAD50 and DNA Damage Response
DNA repair protein RAD50 is a component of the MRN complex (Mre11-RAD50-Nbs1) responsible for DNA double strand break (DSB) repair. DSBs are caused by ionizing radiation, certain chemotherapy drugs, metabolic reactive oxygen species (ROS), replication...  Read full blog post.

The MRE11 Complex and DNA Damage Response
The maintenance of genome stability depends on the DNA damage response (DDR) which is a complex signaling network including cell cycle checkpoints, DNA repair and damage tolerance pathways. The DDR complex has the ability to sense DNA damage and tra...  Read full blog post.

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Gene Symbol RAD50

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