![Immunohistochemistry: Pygopus-1 Antibody [NBP1-42665] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- Pygo1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Pygopus-1-Antibody-Immunohistochemistry-NBP1-42665-img0005.jpg "Immunohistochemistry: Pygopus-1 Antibody [NBP1-42665] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- Pygo1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | IHC, IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit Pygopus-1 Antibody - BSA Free (NBP1-42665) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen for this product maps to a region between residue 300 and 350 of human pygopus homolog 1 using the numbering given in entry NP_056432.1 (GeneID 26108 ). |
| Predicted Species | Mouse (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PYGO1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Immunocytochemistry Pygopus-2 Antibody (539730) [Unconjugated]](https://images.novusbio.com/images2/Pygopus-2_MAB3616_Immunocytochemistry_10465.jpg)
![Western Blot Pygopus-2 Antibody (539730) [Unconjugated]](https://images.novusbio.com/images2/Pygopus-2_MAB3616_Western_Blot_10485.jpg)
![Simple Western Pygopus-2 Antibody (539730) [Unconjugated]](https://images.novusbio.com/images2/Pygopus2_MAB3616_Simple_Western_16406.jpg)
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunoprecipitation: Pygopus-1 Antibody [NBP1-42665] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Pygo1 antibody used for IP at 3 mcg/mg lysate. Pygo1 was also immunoprecipitated by rabbit anti-Pygo1 antibody NBP1-42666, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/Pygopus-1-Antibody-Immunoprecipitation-NBP1-42665-img0004.jpg "Immunoprecipitation: Pygopus-1 Antibody [NBP1-42665] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Pygo1 antibody used for IP at 3 mcg/mg lysate. Pygo1 was also immunoprecipitated by rabbit anti-Pygo1 antibody NBP1-42666, which recognizes a downstream epitope.")
![Western Blot BCL9-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Bcl9-2_AF4967_Western_Blot_5591.jpg)
![Bioactivity FGF18 [Unconjugated]](https://images.novusbio.com/images2/FGF18_8988F18_1072.jpg)
![SDS-PAGE FGF-8 [Unconjugated]](https://images.novusbio.com/images2/FGF-8_423-F8_332.jpg)
![Bioactivity FGF-8 [Unconjugated]](https://images.novusbio.com/images2/FGF8_423F8_1438.jpg)
![Cell Culture FGF-8 [Unconjugated]](https://images.novusbio.com/images/423-f8_recombinant-human-mouse-fgf-8b-protein-cell-culture-1052024145159..jpg)
![Bioactivity FGF-20 [Unconjugated]](https://images.novusbio.com/images/protein/2547-fgcf_recombinant-human-fgf-20-protein-cf-bioactivity-181120209345.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")