Pygopus-2 Antibody (539730) [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse ...read more
Pygopus‑2 was detected in immersion fixed paraffin-embedded sections of normal human ovary (left panel) and ovarian cancer tissue (right panel) using Mouse Anti-Human Pygopus‑2 Monoclonal Antibody (Catalog # ...read more
Pygopus‑2 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human Pygopus-2 Monoclonal Antibody (Catalog # MAB3616) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Pygopus‑2 at approximately 65 kDa (as indicated) using 5 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clone
539730
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Pygopus-2 Antibody (539730) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Pygopus-2
Met160-Glu336
Accession # NP_612157
Specificity
Detects human Pygopus-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Pygopus-1 or recombinant mouse Pygopus-1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PYGO2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pygopus-2 Antibody (539730) [Unconjugated]

  • 1190004M21Rik
  • FLJ33226
  • PYGO2
  • pygopus 2
  • pygopus homolog 2 (Drosophila)
  • pygopus homolog 2
  • Pygopus2
  • Pygopus-2

Background

Human Pygopus-2 (pygopus; the name of a legless Australian lizard) is a 41 kDa, 406 amino acid (aa) transcriptional coactivator.  It contains three domains: an N‑terminal NLS (aa 41-47), followed by a proline-rich region and a zinc-finger PHD-type domain (aa 330-387).  A potential internal alternate start site may generate an isoform that deletes the first 37 aa.  Pygopus 2 is a nuclear protein that acts in concert with BCL-9 and TCF to retain beta -Catenin in the nucleus during Wnt-signaling.  It does so by forming a “chain-of-adaptors” with the above three proteins.  Over the range of aa used for immunization, human pygopus 2 is 95% identical to mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC, IF
NBP1-89679
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB3617
Species: Hu, Mu
Applications: WB
NBP2-24695
Species: Hu
Applications: WB, IHC-P
NBP1-30141
Species: Hu, Mu, Rt, Po, Ce, Dr, In, Ma, Ye
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-14350
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-30472
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-86960
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC, IF
NBP1-51575
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-24613
Species: Hu
Applications: WB, Simple Western, IHC-P
NB600-302
Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45570
Species: Hu
Applications: WB, Flow, IHC
AF6457
Species: Hu
Applications: WB
NB500-487
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P, IP
NBP2-35028
Species: Mu
H00005956-R02
Species: Hu
NBP1-89342
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP

Publications for Pygopus-2 Antibody (MAB3616) (0)

There are no publications for Pygopus-2 Antibody (MAB3616).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Pygopus-2 Antibody (MAB3616) (0)

There are no reviews for Pygopus-2 Antibody (MAB3616). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Pygopus-2 Antibody (MAB3616) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Pygopus-2 Antibody (MAB3616)

Discover related pathways, diseases and genes to Pygopus-2 Antibody (MAB3616). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Pygopus-2 Antibody (MAB3616)

Discover more about diseases related to Pygopus-2 Antibody (MAB3616).
 

Pathways for Pygopus-2 Antibody (MAB3616)

View related products by pathway.

PTMs for Pygopus-2 Antibody (MAB3616)

Learn more about PTMs related to Pygopus-2 Antibody (MAB3616).

Blogs on Pygopus-2

There are no specific blogs for Pygopus-2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Pygopus-2 Antibody (539730) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol PYGO2
Uniprot