BCL9-2 Antibody [Unconjugated]

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Western blot shows lysates of A172 human glioblastoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human Bcl9‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

BCL9-2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Bcl9-2
Met38-Val206
Accession # Q86UU0
Specificity
Detects endogenous human Bcl9-2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
BCL9L
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4967 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BCL9-2 Antibody [Unconjugated]

  • B-cell CLL/lymphoma 9-like protein
  • B-cell CLL/lymphoma 9-like
  • B-cell lymphoma 9-like protein
  • Bcl92
  • Bcl9-2
  • BCL9-like protein
  • DLNB11
  • DLNB11BCL9-2
  • nuclear co-factor of beta-catenin signalling
  • Protein BCL9-2

Background

Bcl9-2 (B-cell lymphoma 9-like protein; also Bcl9L and B9L) is a 200-220 kDa transcriptional regulator that belongs to the Bcl9 family of proteins. It is expressed in multiple tissues, particularly breast, and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl9-2 and Bcl9 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl9-2 is 1499 amino acids (aa) in length. It contains two Pro-rich regions (aa 280-493 and 891-1378). There are four potential alternate start sites at Met879, Met1000, Met708 and Met472. The last two start sites are accompanied by a deletion of aa 1241-1286, and a 62 aa substitution for the C-terminal 167 aa, respectively. Over aa 38-206, human Bcl9-2 is 94% aa identical to mouse Bcl9-2. Bcl9-2 has been identified as a phosphoprotein in several human cancer cell lines and mouse liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BCL9-2 Antibody (AF4967)(2)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 2 applications: ICC, Immunoprecipitation.


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ICC
(1)
Immunoprecipitation
(1)
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(1)
N/A
(1)
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Video Protocols

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Bioinformatics

Gene Symbol BCL9L
Uniprot