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PSTPIP1 Antibody

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Western Blot: PSTPIP1 Antibody [NBP2-93547] - Analysis of extracts of various cell lines, using PSTPIP1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
Western Blot: PSTPIP1 Antibody [NBP2-93547] - Analysis of extracts of various cell lines, using PSTPIP1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug ...read more
Immunohistochemistry-Paraffin: PSTPIP1 Antibody [NBP2-93547] - Paraffin-embedded mouse kidney using PSTPIP1.
Immunohistochemistry-Paraffin: PSTPIP1 Antibody [NBP2-93547] - Paraffin-embedded human stomach using PSTPIP1.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PSTPIP1 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 157-416 of human PSTPIP1 (NP_003969.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSTPIP1
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PSTPIP1 Antibody

  • CD2BP1CD2-binding protein 1
  • CD2BP1L
  • CD2BP1S
  • H-PIPPEST phosphatase-interacting protein 1
  • PAPASCD2 antigen-binding protein 1
  • proline-serine-threonine phosphatase interacting protein 1
  • proline-serine-threonine phosphatase-interacting protein 1
  • PSTPIPCD2 cytoplasmic tail-binding protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-17262
Western Blot: MEFV Antibody [NBP2-17262] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MEFV antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: MEFV Antibody [NBP2-17262] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Western Blot: MEFV Antibody [NBP2-17262] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MEFV antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Species: Hu
Applications: WB, ICC/IF
WASP Antibody
NB300-996
Immunocytochemistry/Immunofluorescence: WASP Antibody [NB300-996] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: WASP Antibody [NB300-996] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: WASP Antibody [NB300-996] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(1 Publication)
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
PTPN12 Antibody
NB100-60666
Western Blot: PTPN12 Antibody [NB100-60666] - Detection of Human and Mouse PTPN12 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PTPN12 antibody NB100-60666 used at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.
Immunohistochemistry: PTPN12 Antibody [NB100-60666] - Sample: FFPE section of human stomach carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti- PTPN12 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: PTPN12 Antibody [NB100-60666] - Detection of Human and Mouse PTPN12/PTP-PEST on HeLa whole cell lysate using NB100-60666. PTPN12/PTPPEST was also IPed by NB100-60663, NB100-60664, and NB100-60665.
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
Reg1A Antibody
NBP2-13215
Immunohistochemistry-Paraffin: Reg1A Antibody [NBP2-13215] - Analysis in human pancreas and kidney tissues using NBP2-13215 antibody. Corresponding REG1A RNA-seq data are presented for the same tissues.
Western Blot: Reg1A Antibody [NBP2-13215] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunohistochemistry-Paraffin: Reg1A Antibody [NBP2-13215] - Staining of human liver shows no positivity in hepatocytes as expected.
Species: Hu
Applications: WB, IHC, IHC-P
NLRP3/NALP3
Antibody
NBP2-12446
Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - C-AR inhibited NLRP3 inflammasome activation in synovial tissue. NLRP3 protein expression in succinate-stimulated synovial fibroblasts. The results were derived from four independent experiments for immunohistochemistry staining and Western blot and expressed as the mean +/- SD. *p < 0.05 vs. the model; #p < 0.05 vs. the indicated treatment. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fimmu.2016.00532/full), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of human esophagus using NLRP3/NALP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in the squamous epithelium was observed. Staining was performed by Histowiz.
Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NLRP3 in cells (Huh-7 and SMMC-7721) exposed luteoloside using anti-NLRP3 antibody. Western blot image submitted by a verified customer review.
(4 Reviews)
(97 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IM, IP, KD
TNF-alpha
[Unconjugated]
210-TA
TNF-alpha [Unconjugated]
(35 Reviews)
(651 Publications)
Species: Hu
PTP pi/PTPRU
Antibody (764209)
MAB7475
PTP pi/PTPRU Antibody (764209)
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Species: Hu, Rt
Applications: WB
SLC25A3 Antibody
H00005250-B02P
Western Blot: SLC25A3 Antibody [H00005250-B02P] - Analysis of SLC25A3 expression in human pancreas.
Immunocytochemistry/Immunofluorescence: SLC25A3 Antibody [H00005250-B02P] - Analysis of purified antibody to SLC25A3 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: SLC25A3 Antibody [H00005250-B02P] - Analysis of SLC25A3 expression in transfected 293T cell line by SLC25A3 polyclonal antibody. Lane 1: SLC25A3 transfected lysate(39.71 KDa). Lane 2: Non-transfected lysate.
Species: Hu
Applications: WB, ICC/IF
ASC/TMS1 Antibody
NBP1-78977
Simple Western: ASC/TMS1 Antibody [NBP1-78977] - Lane view shows a specific band for ASC TMS1 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - NLRP3 activation was evaluated by visualizing the co-localization of NLRP3 and apoptosis-associated speck-like protein (ASC) using immunocytochemistry (A). PLoS One. 2020 Jun 17;15(6):e0234039. doi: 10.1371/journal.pone.0234039. (Catalog # NBP1-78977AF647)
Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Rats were infected by S. pneumoniae or given PBS sham control. 21.5 umoles/kg Mito-Vit-E (MVE) or vehicle was administered orally 30 minutes post-inoculation, and heart tissues were harvested 24 hours later. Heart sections were co-stained with anti-ASC (brown) and haematoxylin (blue). Negative control was stained with secondary antibody alone. Images are representative of a random selection of at least 3 sections of N = 6. PLoS One. 2015 Oct 8;10(10):e0139416. doi: 10.1371/journal.pone.0139416.
(2 Reviews)
(20 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IM, IP
CD2 Antibody
(3D1E3)
NBP2-61727
Western Blot: CD2 Antibody (3D1E3) [NBP2-61727] - Analysis using CD2 mAb against HEK293 (1) and CD2 (AA: 25-140)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: CD2 Antibody (3D1E3) [NBP2-61727] - Analysis of endometrial cancer tissues using CD2 mouse mAb with DAB staining.
Flow Cytometry: CD2 Antibody (3D1E3) [NBP2-61727] - Analysis of HepG2 cells using CD2 mouse mAb (green) and negative control (red).
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Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-P, MiAr
PSTPIP2 Antibody
NBP1-85846
Western Blot: PSTPIP2 Antibody [NBP1-85846] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: PSTPIP2 Antibody [NBP1-85846] - Staining in human spleen and cerebral cortex tissues using anti-PSTPIP2 antibody. Corresponding PSTPIP2 RNA-seq data are presented for the same tissues.
Western Blot: PSTPIP2 Antibody [NBP1-85846] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
c-Abl Antibody
(8E9)
NBP2-49695
Western Blot: c-Abl Antibody (8E9) [NBP2-49695] - Analysis of c-Abl using c-Abl Monoclonal Antibody on HeLa Cells.
Immunocytochemistry/Immunofluorescence: c-Abl Antibody (8E9) [NBP2-49695] - taining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a c-Abl monoclonal antibody in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunohistochemistry-Paraffin: c-Abl Antibody (8E9) [NBP2-49695] - Analysis of human placenta stained with cbl antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Steroid sulfatase
Antibody
NBP1-90095
Immunohistochemistry-Paraffin: Steroid sulfatase Antibody [NBP1-90095] - Staining of human placenta shows high expression.
Immunohistochemistry-Paraffin: Steroid sulfatase Antibody [NBP1-90095] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: Steroid sulfatase Antibody [NBP1-90095] - Staining in human placenta and pancreas tissues using anti-STS antibody. Corresponding STS RNA-seq data are presented for the same tissues.
(3 Publications)
Species: Hu
Applications: IHC, IHC-P
Src Antibody (5A18)
NBP1-19188
Western Blot: Src Antibody (5A18) [NBP1-19188] - Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Src at Y419 and total Src were detected by western blot. From a verified customer review.
Immunohistochemistry: Src Antibody (5A18) [NBP1-19188] - IHC Analysis: Human prostate tissue stained with Src, mAb (5A18) at 20 ug/ml.
Western Blot: Src Antibody (5A18) [NBP1-19188] - Western blot analysis of Src using 20 ug of whole cell lysate (Lane 2=HeLa, Lane 3=3T3, Lane 4=PC12) probed with with Src, mAb (5A18) at 1 ug/ml.
(2 Reviews)
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
Fas Ligand/TNFSF6
[Unconjugated]
126-FL/CF
Fas Ligand/TNFSF6 [Unconjugated]
(17 Publications)
Species: Hu
ERK1 Antibody (1E5)
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
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Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
MVK Antibody
[Unconjugated]
AF8516
MVK Antibody [Unconjugated]
Species: Hu
Applications: WB, Simple Western, IP, ICC

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Bioinformatics

Gene Symbol PSTPIP1