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CD2 Antibody (RPA-2.10)

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Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the PE/Cy5 conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy5 (open histogram) or Anti-Human CD2 PE-Cy5 (filled histogram). Total cells were used for analysis.
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the PE/Cy5 conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy5 (open histogram) or ...read more
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Cell surface analysis of CD2 in 10^6 human lymphocytes using 0.25 ug of CD2 RPA-2.10 antibody; Shaded histogram represents cells without antibody; green represents ...read more
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-25200. Staining of CD2 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram ...read more
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Allophycocyanin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (open ...read more
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Biotin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Biotin (open ...read more
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the FITC conjugate of NBP2-25200. Staining of Human peripheral blood lymphocytes with RPA-2.10 FITC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, Flow, IHC, IHC-Fr
Clone
RPA-2.10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-25200AF405
Alexa Fluor 488
NBP2-25200AF488
Alexa Fluor 647
NBP2-25200AF647
Alexa Fluor 700
NBP2-25200AF700
DyLight 350
NBP2-25200UV
DyLight 405
NBP2-25200V
DyLight 488
NBP2-25200G
DyLight 550
NBP2-25200R
DyLight 650
NBP2-25200C
DyLight 680
NBP2-25200FR
DyLight 755
NBP2-25200IR
FITC
NBP2-25200F
HRP
NBP2-25200H
PerCP
NBP2-25200PCP
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CD2 Antibody (RPA-2.10) Summary

Immunogen
A recombinant protein was used as the immunogen for the CD2 antibody.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Blocking/Neutralizing 50-200 molar excess
  • Flow Cytometry 0.1-0.25 ug per 1x10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Reactivity Notes

This product reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
CD2 RPA-2.10 antibody at 0.5 mg/ml in PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for CD2 Antibody (RPA-2.10)

  • CD2 antigen (p50), sheep red blood cell receptor
  • CD2 antigen
  • CD2 molecule
  • CD2
  • Erythrocyte receptor
  • FLJ46032
  • LFA-2
  • LFA-3 receptor
  • lymphocyte-function antigen-2
  • Rosette receptor
  • SRBC
  • T11
  • T-cell surface antigen CD2
  • T-cell surface antigen T11/Leu-5

Background

CD2 a well known Tcell marker is a surface antigen of the human T-lymphocyte lineage. Also known as the sheep erythrocyte receptor, CD2 is a 351 amino acid containing protein that contains an Ig-like C2 -type (immunoglobulin like) domain, an Ig-like V -type (immunoglobulin like) domain and a cytoplasmic domain implicated in Tcell signaling. It interacts with CD2AP (CD2-associated protein) and PSTPIP1 (proline-serine-threonine phosphatase interacting protein 1). CD2 mediates adhesion between Tcells and other cell types by interacting with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1. Interaction between CD2 and its counter receptor, LFA3 (CD58), on opposing cells helps in immune recognition and facilitates contacts between helper T-lymphocytes and antigen-presenting cells as well as between cytolytic effectors and target cells. CD2 expression promotes chronic HBV infection and may also contribute to the hepatocyte damage. Expression of CD2 is common on all peripheral blood Tcells, thymocytes, and also on some natural killer cells but not on B lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
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Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Analysis of CD3+ hPMBCs using the APC conjugate of this antibody. A cell surface stain was performed on CD3+ hPBMCs with V alpha 24 J alpha 18 TCR antibody (6B11) NBP2-00267PE (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. A co-stain was also performed using CD3 antibody NBP2-24867APC.
Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Surface staining of human PBMC with CD3 antibody (red) and isotype control (green) at 0.2 ug/10^6 cells.
Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Surface staining of human PBMC with CD3 antibody (red) grown in serum-free media and isotype control (green) at 0.25 ug/10^6 cells.
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Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human CTLA-4. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CTLA‑4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NS0 mouse myeloma cell line co-transfected with human CTLA-4 and eGFP was stained with either (A) Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
CTLA‑4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
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Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
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Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.
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Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).
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Immunocytochemistry/Immunofluorescence: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 (green) showing staining in the cytoplasm of Raji cells (right) compared to a negative control without primary antibody (left).
Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 in BAF-3 cells compared to an isotype control (blue).
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Immunohistochemistry-Frozen: CD45 Antibody (30-F11) [NB100-77417] - IHC analysis of a mouse spleen cryosection using CD45 antibody clone 30-F11 at 1:50 dilution. The signal was detected using goat anti-rat Alexa fluor 488 secondary with DAPI counterstaining. Image submitted via a verified customer review.
Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with CD45 antibody (NB100-77417) at 1:1000 dilution and detected with FITC conjugated donkey anti-rat IgG secondary (orange). Shown with rat IgG isotype control (blue).
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Immunocytochemistry/Immunofluorescence: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of monoclonal antibody to ISG20 on HeLa cell. Antibody concentration 10 ug/ml.
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Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with CD9 (HI9a) antibody NB100-77915AF647 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646.
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Surface staining of 10^6 human lymphocytes using 0.2 ug of CD19 antibody (red) and isotype control (green). Cell surface staining kit was used for this test.
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CD2 Antibody
(RPA-2.10)
NBP2-25200
CD2 Antibody (RPA-2.10)
Species: Hu
Applications: WB, B/N, Flow, IHC, IHC-Fr
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-25200AF405
Alexa Fluor 488 Labeled NBP2-25200AF488
Alexa Fluor 647 Labeled NBP2-25200AF647
Alexa Fluor 700 Labeled NBP2-25200AF700
DyLight 350 Labeled NBP2-25200UV
DyLight 405 Labeled NBP2-25200V
DyLight 488 Labeled NBP2-25200G
DyLight 550 Labeled NBP2-25200R
DyLight 650 Labeled NBP2-25200C
DyLight 680 Labeled NBP2-25200FR
DyLight 755 Labeled NBP2-25200IR
HRP Labeled NBP2-25200H
PerCP Labeled NBP2-25200PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol CD2
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