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CD2 Antibody (RPA-2.10)

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Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - CD3+ T-Cells isolated from PBMCs stained with anti-CD2 Alexa Fluor 488 (BL1). Image from a verified customer review.
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - CD3+ T-Cells isolated from PBMCs stained with anti-CD2 Alexa Fluor 488 (BL1). Image from a verified customer review.
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Cell surface analysis of CD2 in 10^6 human lymphocytes using 0.25 ug of CD2 RPA-2.10 antibody; Shaded histogram represents cells without antibody; green represents ...read more
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-25200. Staining of CD2 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram ...read more
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Allophycocyanin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (open ...read more
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Biotin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Biotin (open ...read more
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the FITC conjugate of NBP2-25200. Staining of Human peripheral blood lymphocytes with RPA-2.10 FITC.
Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the PE/Cy5 conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy5 (open histogram) or ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, B/N
Clone
RPA-2.10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-25200AF350
Alexa Fluor 405
NBP2-25200AF405
Alexa Fluor 488
NBP2-25200AF488
Alexa Fluor 532
NBP2-25200AF532
Alexa Fluor 594
NBP2-25200AF594
Alexa Fluor 647
NBP2-25200AF647
Alexa Fluor 700
NBP2-25200AF700
Alexa Fluor 750
NBP2-25200AF750
DyLight 350
NBP2-25200UV
DyLight 405
NBP2-25200V
DyLight 488
NBP2-25200G
DyLight 550
NBP2-25200R
DyLight 594
NBP2-25200DL594
DyLight 650
NBP2-25200C
DyLight 680
NBP2-25200FR
DyLight 755
NBP2-25200IR
FITC
NBP2-25200F
HRP
NBP2-25200H
PerCP
NBP2-25200PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-80635
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CD2 Antibody (RPA-2.10) Summary

Immunogen
A recombinant protein was used as the immunogen for the CD2 antibody.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 0.1-0.25 ug per 1x10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Block/Neutralize
Application Notes
Flow reported from a verified customer review.

Reactivity Notes

This product reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
CD2 RPA-2.10 antibody at 0.5 mg/ml in PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for CD2 Antibody (RPA-2.10)

  • CD2 antigen (p50), sheep red blood cell receptor
  • CD2 antigen
  • CD2 molecule
  • CD2
  • Erythrocyte receptor
  • FLJ46032
  • LFA-2
  • LFA-3 receptor
  • lymphocyte-function antigen-2
  • Rosette receptor
  • SRBC
  • T11
  • T-cell surface antigen CD2
  • T-cell surface antigen T11/Leu-5

Background

CD2 a well known Tcell marker is a surface antigen of the human T-lymphocyte lineage. Also known as the sheep erythrocyte receptor, CD2 is a 351 amino acid containing protein that contains an Ig-like C2 -type (immunoglobulin like) domain, an Ig-like V-type (immunoglobulin like) domain and a cytoplasmic domain implicated in Tcell signaling. It interacts with CD2AP (CD2-associated protein) and PSTPIP1 (proline-serine-threonine phosphatase interacting protein 1). CD2 mediates adhesion between Tcells and other cell types by interacting with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1. Interaction between CD2 and its counter receptor, LFA3 (CD58), on opposing cells helps in immune recognition and facilitates contacts between helper T-lymphocytes and antigen-presenting cells as well as between cytolytic effectors and target cells. CD2 expression promotes chronic HBV infection and may also contribute to the hepatocyte damage. Expression of CD2 is common on all peripheral blood Tcells, thymocytes, and also on some natural killer cells but not on B lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
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Immunocytochemistry/Immunofluorescence: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 (green) showing staining in the cytoplasm of Raji cells (right) compared to a negative control without primary antibody (left).
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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-25200AF350
Alexa Fluor 405 NBP2-25200AF405
Alexa Fluor 488 NBP2-25200AF488
Alexa Fluor 532 NBP2-25200AF532
Alexa Fluor 594 NBP2-25200AF594
Alexa Fluor 647 NBP2-25200AF647
Alexa Fluor 700 NBP2-25200AF700
Alexa Fluor 750 NBP2-25200AF750
DyLight 350 NBP2-25200UV
DyLight 405 NBP2-25200V
DyLight 488 NBP2-25200G
DyLight 550 NBP2-25200R
DyLight 594 NBP2-25200DL594
DyLight 650 NBP2-25200C
DyLight 680 NBP2-25200FR
DyLight 755 NBP2-25200IR
FITC NBP2-25200F
HRP NBP2-25200H
PerCP NBP2-25200PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol CD2