PTP pi/PTPRU Antibody (764209)

Western blot shows lysates of human brain (cerebellum) tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PTPπ/PTPRU Monoclonal Antibody (Catalog # MAB7475) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PTPπ/PTPRU at approximately 180-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Rt
• Applications: WB
• Clone: 764209
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PTP pi/PTPRU Antibody (764209) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PTPπ/PTPRU
  Glu19-Gln740
  Accession # Q92729
• Specificity: Detects human and rat PTPπ/PTPRU in Western blots and recombinant human PTPRU in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PTPRK, rhPTPRM, or rhPTPRT is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRU
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTP pi/PTPRU Antibody (764209)

• FMI
• GLEPP1
• hPTP-J
• pancreatic carcinoma phosphatase 2
• PCP2
• PCP-2
• pi R-PTP-Psi
• protein tyrosine phosphatase J
• protein tyrosine phosphatase, receptor type, U
• protein-tyrosine phosphatase J
• protein-tyrosine phosphatase pi
• PTP pi
• PTP
• PTP-J
• PTP-PI
• PTPPSI
• PTPRO
• PTP-RO
• PTPRU
• Receptor protein tyrosine phosphatase hPTP-J
• R-PTP-PSI
• R-PTP-U

Background

Protein Tyrosine Phosphatase, receptor type U (PTPRU), also called PCP-2 or PTPp, is an approximately 180 kDa type I transmembrane glycoprotein that is a type IIb receptor-like protein tyrosine phosphatase (PTPR). The 731 amino acid (aa) extracellular domain contains MAM, Ig-like C-type and Fibronectin III domains that often mediate cell adhesion, but unlike other family members, PTPRU does not appear to mediate homophilic cell aggregation. It does, however, antagonize Wnt signaling in adherens junctions by inhibiting beta -catenin-mediated gene transcription. Human PTPRU shares 95% aa sequence identity with mouse and rat PTPRU within the extracellular domain, and up to 60% aa identity with other human family members. Isoforms of 1436, 1440 and 1433 aa are altered within the cytoplasmic domain as compared to the 1446 aa full-length form. Several tissues contain detectable amounts of PTPRU mRNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTP pi/PTPRU Antibody (MAB7475)(1)

Publication using MAB7475

• WA Rashad, S Sakr, AM Domouky Comparative study of oral versus parenteral crocin in mitigating acrolein-induced lung injury in albino rats Oncogene, 2022-06-17;12(1):10233. 2022-06-17 [PMID: 35715565] (ELISA Detection, Rat)

Bioinformatics

• Gene Symbol: PTPRU
• Uniprot:
  • Q92729(Human)