PTP pi/PTPRU Antibody (764209)

Western blot shows lysates of human brain (cerebellum) tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PTPp/PTPRU Monoclonal Antibody (Catalog # MAB7475) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PTPp/PTPRU at approximately 180-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

An immunoblot analysis was performed to determine the expression levels of EPAS1, PTPRU and beta -catenin in response to miR-184 or miR-574-5p in SCLC cell linesA. miR-184 overexpression inhibited EPAS1 and beta -catenin in H446 and H2227 cells, whereas miR-184 inhibition had the opposite effects. B. miR-574-5p overexpression inhibited PTPRU and enhanced the tyrosine phosphorylation of beta -catenin in H446 and H2227 cells, whereas miR-574-5p inhibition had the opposite effects. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26587830), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Rt
• Applications: WB
• Clone: 764209
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PTP pi/PTPRU Antibody (764209) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PTPπ/PTPRU
  Glu19-Gln740
  Accession # Q92729
• Specificity: Detects human and rat PTPπ/PTPRU in Western blots and recombinant human PTPRU in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PTPRK, rhPTPRM, or rhPTPRT is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRU
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTP pi/PTPRU Antibody (764209)

• FMI
• GLEPP1
• hPTP-J
• pancreatic carcinoma phosphatase 2
• PCP2
• PCP-2
• pi R-PTP-Psi
• protein tyrosine phosphatase J
• protein tyrosine phosphatase, receptor type, U
• protein-tyrosine phosphatase J
• protein-tyrosine phosphatase pi
• PTP pi
• PTP
• PTP-J
• PTP-PI
• PTPPSI
• PTPRO
• PTP-RO
• PTPRU
• Receptor protein tyrosine phosphatase hPTP-J
• R-PTP-PSI
• R-PTP-U

Background

Protein Tyrosine Phosphatase, receptor type U (PTPRU), also called PCP-2 or PTPp, is an approximately 180 kDa type I transmembrane glycoprotein that is a type IIb receptor-like protein tyrosine phosphatase (PTPR). The 731 amino acid (aa) extracellular domain contains MAM, Ig-like C-type and Fibronectin III domains that often mediate cell adhesion, but unlike other family members, PTPRU does not appear to mediate homophilic cell aggregation. It does, however, antagonize Wnt signaling in adherens junctions by inhibiting beta -catenin-mediated gene transcription. Human PTPRU shares 95% aa sequence identity with mouse and rat PTPRU within the extracellular domain, and up to 60% aa identity with other human family members. Isoforms of 1436, 1440 and 1433 aa are altered within the cytoplasmic domain as compared to the 1446 aa full-length form. Several tissues contain detectable amounts of PTPRU mRNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTP pi/PTPRU Antibody (MAB7475)(4)

Publications using MAB7475

• WA Rashad, S Sakr, AM Domouky Comparative study of oral versus parenteral crocin in mitigating acrolein-induced lung injury in albino rats Oncogene, 2022-06-17;12(1):10233. 2022-06-17 [PMID: 35715565] (ELISA Detection, Rat)
• Yang Bai, Yun Wang, Ming Liu, Yu-Han Gu, Bin Jiang, Xu Wu et al. Suppression of nuclear factor erythroid-2-related factor 2-mediated antioxidative defense in the lung injury induced by chronic exposure to methamphetamine in rats Molecular Medicine Reports 2017-05-01 [PMID: 28339044]
• Rui Zhou, Xiaoshu Zhou, Zhongyuan Yin, Jing Guo, Ting Hu, Shun Jiang et al. MicroRNA-574-5p promotes metastasis of non-small cell lung cancer by targeting PTPRU Scientific Reports 2016-10-20 [PMID: 27761023] (Western Blot, Human)
• Rui Zhou, Xiaoshu Zhou, Zhongyuan Yin, Jing Guo, Ting Hu, Shun Jiang et al. Tumor invasion and metastasis regulated by microRNA-184 and microRNA-574-5p in small-cell lung cancer Oncotarget 2015-12-29 [PMID: 26587830]

Bioinformatics

• Gene Symbol: PTPRU
• Uniprot:
  • Q92729(Human)