PTPN12 Antibody

Images

 
Western Blot: PTPN12 Antibody [NB100-60666] - Detection of Human and Mouse PTPN12 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified ...read more
Immunohistochemistry: PTPN12 Antibody [NB100-60666] - Sample: FFPE section of human stomach carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti- PTPN12 used at a dilution of 1:200 ...read more
Independent Antibodies: Western Blot: PTPN12 Antibody [NB100-60666] - Detection of Human and Mouse PTPN12/PTP-PEST on HeLa whole cell lysate using NB100-60666. PTPN12/PTPPEST was also IPed by NB100-60663, ...read more
Immunohistochemistry-Paraffin: PTPN12 Antibody [NB100-60666] - Sample: FFPE section of human colon carcinoma. Antibody used at a dilution of 1:250.
Immunohistochemistry-Paraffin: PTPN12 Antibody [NB100-60666] - Sample: FFPE section of mouse renal cell carcinoma. Antibody used at a dilution of 1:250.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

PTPN12 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 760 and 780 of human protein tyrosine phosphatase, non-receptor type 12 using the numbering given in entry NP_002826.2 (GeneID 5782).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTPN12
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
PTPN12 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PTPN12 Antibody

  • protein tyrosine phosphatase, non-receptor type 12
  • Protein-tyrosine phosphatase G1
  • PTPG1
  • PTPG1tyrosine-protein phosphatase non-receptor type 12
  • PTPN12
  • PTP-PEST
  • PTP-PESTEC 3.1.3.48

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may be related to protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. This PTP was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol PTPN12
Entrez
Uniprot