PSAP Antibody (4D5F4) - BSA Free

Western Blot: PSAP Antibody (4D5F4) [NBP2-37422] - Western blot analysis using PSAP mAb against HEK293 (1) and PSAP (AA: 325-524)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: PSAP Antibody (4D5F4) [NBP2-37422] - Immunofluorescence analysis of HepG2 cells using PSAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: PSAP Antibody (4D5F4) [NBP2-37422] - Analysis of pancreas tissues using PSAP mouse mAb with DAB staining.

Flow Cytometry: PSAP Antibody (4D5F4) [NBP2-37422] - Flow cytometric analysis of HeLa cells using PSAP mouse mAb (green) and negative control (purple).

Western Blot: PSAP Antibody (4D5F4) [NBP2-37422] - Western blot analysis using PSAP mAb against human PSAP recombinant protein. (Expected MW is 47.8 kDa)

ELISA: PSAP Antibody (4D5F4) [NBP2-37422] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 4D5F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PSAP Antibody (4D5F4) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human PSAP (AA: 325-524) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSAP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 58.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PSAP Antibody (4D5F4) - BSA Free

• FLJ00245
• GLBA
• proactivator polypeptide
• prosaposin
• PSAP
• SAP1
• SAP1MGC110993
• sphingolipid activator protein-1

Background

PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D, which are similar to each other and are sphingolipid hydrolase activator proteins. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in PSAP gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSAP