ELK3 Antibody (OTI1H3)

Images

 
Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264] - Breast cancer cells. Lane 1: MDA-MB231 + Negative control siRNA. Lane 2: MDA-MB231 + siELK3-1. Lane 3: MDA-MB231 + siELK3-2. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: ELK3 Antibody (1H3) [NBP2-01264] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ELK3.
Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Human tonsil using anti-ELK3 mouse monoclonal antibody.
Flow Cytometry: ELK3 Antibody (1H3) [NBP2-01264] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ELK3 antibody, and then analyzed by ...read more
Western Blot: ELK3 Antibody (1H3) [NBP2-01264] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ELK3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ELK3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ELK3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Human lymphoma tissue using anti-ELK3 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI1H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ELK3 Antibody (OTI1H3) Summary

Immunogen
Full length human recombinant protein of human ELK3(NP_005221) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ELK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
44.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-01264 in the following applications:

Publications
Read Publications using
NBP2-01264 in the following applications:

  • WB
    2 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ELK3 Antibody (OTI1H3)

  • ELK3, ETS-domain protein (SRF accessory protein 2)
  • ERPETS domain-containing protein Elk-3
  • ETS-domain protein
  • ETS-related protein ERP
  • NETSRF accessory protein 2
  • SAP-2
  • SAP2ETS-related protein NET
  • Serum response factor accessory protein 2

Background

The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ELK3 Antibody (NBP2-01264)(3)

Review for ELK3 Antibody (NBP2-01264) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-01264:
Filter by Applications
Western Blot
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot ELK3 NBP2-01264
Enlarge
5
reviewed by:
Verified Customer
Western Blot Human 07/17/2018
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Summary

ApplicationWestern Blot
Sample TestedBreast cancer cells
SpeciesHuman
LotA001

Comments

Comments1 : MDA-MB231 + Negative control siRNA
2 : MDA-MB231 + siELK3-1
3 : MDA-MB231 + siELK3-2

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ELK3 Antibody (NBP2-01264) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
07/17/2018
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol ELK3
Entrez