ELK3 Antibody (OTI1H3)

Images

 
Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264] - Breast cancer cells. Lane 1: MDA-MB231 + Negative control siRNA. Lane 2: MDA-MB231 + siELK3-1. Lane 3: MDA-MB231 + siELK3-2. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: ELK3 Antibody (1H3) [NBP2-01264] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ELK3.
Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Human tonsil using anti-ELK3 mouse monoclonal antibody.
Flow Cytometry: ELK3 Antibody (1H3) [NBP2-01264] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ELK3 antibody, and then analyzed by ...read more
Western Blot: ELK3 Antibody (1H3) [NBP2-01264] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ELK3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ELK3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ELK3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Human lymphoma tissue using anti-ELK3 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI1H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.85 mg/ml

Order Details

ELK3 Antibody (OTI1H3) Summary

Immunogen
Full length human recombinant protein of human ELK3(NP_005221) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ELK3
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
44.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-01264 in the following applications:

Publications
Read Publication using NBP2-01264.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.85 mg/ml
Purity
Affinity purified

Alternate Names for ELK3 Antibody (OTI1H3)

  • ELK3, ETS-domain protein (SRF accessory protein 2)
  • ERPETS domain-containing protein Elk-3
  • ETS-domain protein
  • ETS-related protein ERP
  • NETSRF accessory protein 2
  • SAP-2
  • SAP2ETS-related protein NET
  • Serum response factor accessory protein 2

Background

The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ELK3 Antibody (NBP2-01264)(1)

We have publications tested in 1 confirmed species: Human.


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(1)
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Review for ELK3 Antibody (NBP2-01264) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-01264:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot ELK3 NBP2-01264
Enlarge
reviewed by:
JIHOON PARK
WB Human 07/17/2018
View

Summary

ApplicationWestern Blot
Sample TestedBreast cancer cells
SpeciesHuman
LotA001

Comments

Comments1 : MDA-MB231 + Negative control siRNA
2 : MDA-MB231 + siELK3-1
3 : MDA-MB231 + siELK3-2

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ELK3 Antibody (NBP2-01264) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70643AF350
Alexa Fluor 405 NBP2-70643AF405
Alexa Fluor 488 NBP2-70643AF488
Alexa Fluor 532 NBP2-70643AF532
Alexa Fluor 594 NBP2-70643AF594
Alexa Fluor 647 NBP2-70643AF647
Alexa Fluor 700 NBP2-70643AF700
Alexa Fluor 750 NBP2-70643AF750
Allophycocyanin NBP2-70643APC
Biotin NBP2-70643B
DyLight 350 NBP2-70643UV
DyLight 405 NBP2-70643V
DyLight 488 NBP2-70643G
DyLight 550 NBP2-70643R
DyLight 594 NBP2-70643DL594
DyLight 650 NBP2-70643C
DyLight 680 NBP2-70643FR
DyLight 755 NBP2-70643IR
FITC NBP2-70643F
HRP NBP2-70643H
Janelia Fluor 549 NBP2-70643JF549
Janelia Fluor 646 NBP2-70643JF646
PE NBP2-70643PE
PerCP NBP2-70643PCP

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Recent Reviews

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JIHOON PARK
07/17/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol ELK3
Entrez
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