Arylsulfatase A/ARSA Antibody

Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Western blot analysis of Mouse testes lysate (A) + peptide (B) with antibody at 1 ug/mL and Rat testes lysate (C) + peptide (D) with antibody at 0.3 ug/mL, 35ug protein in RIPA buffer. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Arylsulfatase A/ARSA Antibody Summary

• Immunogen: Peptide with sequence C-YDLSKDPGENYN corresponding to internal region according to NP_000478.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARSA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: Approx 60-65 kDa band observed in Mouse and Rat Testis lysates (calculated MW of 53.7 kDa according to Mouse NP_033843.2 and Rat NP_001030105.2;). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunizing peptide.
• Control:

  Arylsulfatase A/ARSA Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Arylsulfatase A/ARSA Antibody

• ARSA
• Arylsulfatase A
• ASA
• Cerebroside 3-sulfatase
• Cerebroside-sulfatase
• EC 3.1.6
• EC 3.1.6.8
• MLD

Background

The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Multiple alternatively spliced transcript variants, one of which encodes a distinct protein, have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Arylsulfatase A/ARSA Antibody (NBP1-00154)(1)

Publication using NBP1-00154

• Sevin C, Benraiss A, Van Dam D et al. Intracerebral adeno-associated virus-mediated gene transfer in rapidly progressive forms of metachromatic leukodystrophy. Hum Mol Genet 2006-01-01 [PMID: 16311251]

Bioinformatics

• Gene Symbol: ARSA
• Entrez:
  • 410(Human)
  • 11883(Mouse)
  • 315222(Rat)
• Uniprot:
  • NP_000478.2(Human)