Protein Disulfide Isomerase/P4HB Antibody - BSA Free

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining in human small intestine and testis tissues.. Corresponding P4HB RNA-seq data are presented for the same tissues.

Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human testis shows no cytoplasmic positivity in cells in seminiferous ducts as expected.

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.

Analysis in human cell line HepG2.

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Protein Disulfide Isomerase/P4HB Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Protein Disulfide Isomerase/P4HB Antibody - BSA Free (NBP1-84051) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Protein Disulfide Isomerase/P4HB Recombinant Protein corresponding to amino acids: LPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNF
• Marker: Fibroblast Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: P4HB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Protein Disulfide Isomerase/P4HB Recombinant Protein Antigen (NBP1-84051PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Protein Disulfide Isomerase/P4HB Antibody - BSA Free

• Cellular thyroid hormone-binding protein
• collagen prolyl 4-hydroxylase beta
• DSI
• ERBA2L
• GIT
• glutathione-insulin transhydrogenase
• P4HB
• P4Hbeta
• p55
• PDI
• PDIA1
• PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
• PDIEC 5.3.4.1
• PHDB
• PO4DB
• PO4HB
• procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
• PROHB
• PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
• Prolyl 4-hydroxylase subunit beta
• prolyl 4-hydroxylase, beta polypeptide
• protein disulfide isomerase family A, member 1
• protein disulfide isomerase/oxidoreductase
• protein disulfide isomerase-associated 1
• protein disulfide-isomerase
• protocollagen hydroxylase
• Thbp
• thyroid hormone-binding protein p55

Background

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Protein Disulfide Isomerase/P4HB Antibody (NBP1-84051). (Showing 1 - 1 of 1 FAQ).

1. We purchased  Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] and are wondering if it's specific for the monomeric version of the protein or the tetrameric version? We believe this antibody is staining primary cells (isolated from mice) that are not fibroblasts. Do you know if this antibody can stain primary cells that are not fibroblasts?
   • Our lab has not tested this Ab with the monomeric or multimeric subunits of the protein targets.  However, the immunogen for NBP1-84051 is a.a. 236 - 379 of mouse protein (Uniprot/P09103) and  since it is a polyclonal antibody (multiple epitopes), there are very strong chances that it could detect multimers also. Our lab has performed ICC/IF staining with NBP1-84051 on human U-2 OS cell line and we have not  tested murine primary cells or fibroblasts yet. However, we strongly believe thatthis antibody will detect P4HB in any tissues/organs, cell lines/primary cells and fibroblasts as long as they express this protein.

Bioinformatics

• Gene Symbol: P4HB