TPPP/p25 Antibody

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Analysis in human cerebral cortex and liver tissues using NBP2-34031 antibody. Corresponding TPPP RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human cerebral cortex, liver, lower gastrointestinal and pancreas using Anti-TPPP antibody NBP2-34031 (A) shows similar protein distribution across tissues to independent antibody NBP1-80962 (B).

Western Blot: TPPP/p25 Antibody [NBP2-34031] - TPPP/p25, a microtubule-stabilising protein, is a target of linc00899 in regulation of mitosis. Representative western blot of TPPP/p25 levels after depletion of linc00899. beta-Tubulin and p150 were used as two loading controls. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-14978-7), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: TPPP/p25 Antibody [NBP2-34031] - Staining of human cell line A549 shows localization to cytosol & mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human liver shows no positivity in hepatocytes as expected.

Western Blot: TPPP/p25 Antibody [NBP2-34031] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral cortex tissue.

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in glial cells.

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human lower gastrointestinal shows weak cytoplasmic positivity in peripheral ganglion.

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TPPP/p25 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: AKKRFKDKSSEEAVREVHRLIEGKAPIISGVTKAISSPTVSRLTDTTKFTGSHK
• Predicted Species: Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TPPP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TPPP/p25 Protein (NBP2-34031PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TPPP/p25 Antibody

• p24
• P24,25 kDa brain-specific protein
• p25
• p25alpha
• p25-alpha
• TPPP/p25brain specific protein p25 alpha
• TPPP1glycogen synthase kinase 3 (GSK3) inhibitor p24
• tubulin polymerization promoting protein
• tubulin polymerization-promoting protein

Background

TPPP may play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity ofthe microtubule network. May play a role in mitotic spindle assembly and nuclear env

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TPPP/p25 Antibody (NBP2-34031)(1)

Publication using NBP2-34031

• Stojic L, Lun ATL, Mascalchi P et al. A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division Nat Commun 2020-04-15 [PMID: 32296040] (WB, Human)

Bioinformatics

• Gene Symbol: TPPP
• Uniprot:
  • O94811(Human)