Progranulin/PGRN Antibodies

HAF016). A specific band was detected for Progranulin/PGRN at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for Progranulin/PGRN at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Progranulin/PGRN at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF019). A specific band was detected for Progranulin/PGRN at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF019). A specific band was detected for Progranulin/PGRN at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Progranulin/PGRN at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF017). A specific band was detected for Progranulin/PGRN at approximately 80 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for Progranulin/PGRN at approximately 80 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Progranulin was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse Progranulin Monoclonal Antibody (Catalog # MAB25571) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF018). A specific band was detected for Progranulin/PGRN at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. For Western blotting, Catalog # AF2420is recommended." class="big_lightbox"> HAF018). A specific band was detected for Progranulin/PGRN at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. For Western blotting, Catalog # AF2420is recommended." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Progranulin/PGRN Polyclonal Antibody (Catalog # MAB24201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Progranulin/PGRN at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. For Western blotting, Catalog # AF2420is recommended." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

DY990). Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB25572) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB25572) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Mouse Progranulin/PGRN protein was serially diluted 2-fold and captured by Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB2557R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB25572) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

MAB2557R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB25572) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB2557R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB25572) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Mouse Progranulin/PGRN protein was serially diluted 2-fold and captured by Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB2557R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB25572) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

DY990). Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Progranulin/PGRN protein was serially diluted 2-fold and captured by Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24203) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

MAB24203) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB24203) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Progranulin/PGRN protein was serially diluted 2-fold and captured by Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24203) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />