Lyp/PTPN22 Antibody [Unconjugated]

Images

 
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell lines. PVDF membrane was probed with 0.3 µg/mL of Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal ...read more
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lyp at approximately 116-118 kDa (as ...read more
Lyp was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3428) at 15 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Lyp/PTPN22 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Lyp
Ser306-Ser684
Accession # Q9Y2R2
Specificity
Detects human Lyp in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PTPN22
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 ug/mL
  • Simple Western 3 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF3428 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lyp/PTPN22 Antibody [Unconjugated]

  • EC 3.1.3.48
  • Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP
  • Lymphoid phosphatase
  • lymphoid-specific protein tyrosine phosphatase
  • Lyp
  • Lyp1
  • Lyp2
  • LyPTP
  • PEP
  • PEST-domain phosphatase
  • protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
  • protein tyrosine phosphatase, non-receptor type 8
  • PTPN22
  • PTPN8 (former)
  • PTPN8LYP1
  • tyrosine-protein phosphatase non-receptor type 22

Background

Lymphoid phosphatase (Lyp), also called LyPTP, Protein Tyrosine Phosphatase, Nonreceptor-type 22 (PTPN22), and PEST-domain Phosphatase (PEP), dephosphorylates tyrosine residues in proteins. Found primarily in bone marrow and lymphoid cell lines, an R620W gain-of-function mutation in Lyp has been associated with susceptibility to autoimmune diseases such as type I diabetes, rheumatoid arthritis, and lupus erythematosus. Mature PEP knockout mice have an excess of CD8 positive T-cells and an exaggerated antigen-induced proliferative response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lyp/PTPN22 Antibody (AF3428)(2)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol PTPN22
Uniprot