Profilin 1 Antibody - BSA Free

Western Blot: Profilin 1 Antibody [NBP1-19344] - Western Blot on HeLa whole cell extracts.

Immunohistochemistry: Profilin 1 Antibody [NBP1-19344] - Staining of Profilin in mouse thymus.

Simple Western: Profilin 1 Antibody [NBP1-19344] - Simple Western lane view shows a specific band for Profilin 1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.17 mg/ml

Profilin 1 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to a C-terminal portion of human Profilin 1 (within residues 100-140). [Swiss-Prot# P07737]
• Localization: Cytoplasm, Cytoskeleton.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PFN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 1:200
  • Western Blot 0.5 ug/ml
• Application Notes: This Profilin 1 antibody is useful for Immunohistochemistry on formalin fixed paraffin embedded sections and Western blot, where a band is seen at approx. 14 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:200, apparent MW was 18 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  Profilin 1 Peptide (NBP1-19344PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1.17 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Profilin 1 Antibody - BSA Free

• PFN1
• PROF1
• Profilin 1
• Profilin I
• profilin-1

Background

Profilin-1 (PFN1) is a widely expressed actin-binding protein that implicates in diverse cellular activities ranging from actin polymerization regulation to control of gene transcription. PFN1 facilitate actin polymerization and cell movement by catalyzing the exchange of actin-bound ADP for ATP, and by liberating actin monomer (G-actin) from its sequestering protein, thymosin-beta4; both processes leading to unidirectional addition of G-actin to elongating actin filaments. PFN1 binds actin for impacting cytoskeletal structures, and interestingly, at high concentrations, PFN1 prevents polymerization of actin, and vice versa. PFN1-PIP2 binding inhibits the formation of IP3 and DG, and PFN1 also inhibits AR/HTT aggregation via G-actin binding. Indeed, PFN1 phosphorylation at Ser-138 site has been shown to reduce its affinity for G-actin that follows inhibition of PFN1/HTT interactions and AR/HTT aggregation. PFN1 has long been considered an essential control element for actin polymerization and cell migration, however, recent data suggests an inhibitory action of PFN1 on motility of certain types of carcinoma cells. PFN1 gene disruption causes gross impairment of development and embryonic lethality in insects/mice models abd defects in PFN1 have been linked to amyotrophic lateral sclerosis 18 (ALS18)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Profilin 1 Antibody (NBP1-19344)(1)

Publication using NBP1-19344

• van Blitterswijk M, Baker MC, Bieniek KF et al. Profilin-1 mutations are rare in patients with amyotrophic lateral sclerosis and frontotemporal dementia. Amyotroph Lateral Scler Frontotemporal Degener 2013-05-02 [PMID: 23634771] (IHC-P, Human)

FAQs for Profilin 1 Antibody (NBP1-19344). (Showing 1 - 1 of 1 FAQ).

1. We have stained human oral tissue with your PFN1 monoclonal antibody NBP1-19344. Is the antibody specific to PFN1 or does it also bind to PFN2, and if so how well?
   • Unfortunately we have not tested NBP1-19344 against purified PFN2. I have however aligned the immunogen against the human PFN2 protein for you, and found the homology to be just 45%. We usually suggest that a sequence homology of greater than 80% is indicative of likely binding, although without further testing being carried out I cannot say for certain that this antibody would not recognise PFN2.

Bioinformatics

• Gene Symbol: PFN1