Gelsolin/GSN Antibody (893205) [Unconjugated]

MAB8170) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." > MAB8170) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." title="Gelsolin/GSN was detected in immersion fixed paraffin-embedded sections of human Renal Cell Carcinoma using Mouse Anti-Human Gelsolin/GSN, pan Monoclonal Antibody (Catalog # MAB8170) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." />

Gelsolin/GSN was detected in immersion fixed paraffin-embedded sections of human Renal Cell Carcinoma using Mouse Anti-Human Gelsolin/GSN, pan Monoclonal Antibody (Catalog # <a class=

HAF018). A specific band was detected for Gelsolin/GSN at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF018). A specific band was detected for Gelsolin/GSN at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Gelsolin/GSN at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (U‑87 MG) (NBP2-49844) and SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Gelsolin/GSN at approximately 90 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and distal convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and distal convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="Gelsolin/GSN was detected in formalin fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and distal convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

Gelsolin/GSN was detected in formalin fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Western blot shows lysates of U2OS human osteosarcoma cell line and Gelsolin/GSN knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Gelsolin/GSN at approximately 80 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

• Applications: WB, Simple Western, IHC, KO, mIF
• Clone: 893205
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Gelsolin/GSN Antibody (893205) [Unconjugated] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human Gelsolin/GSN
  Met1-Ala782
  Accession # P06396
• Specificity: Detects human Gelsolin/GSN in ELISAs. Detects human, mouse and rat Gelsolin/GSN in Western Blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Knockout Validated
  • Multiplex Immunofluorescence 20 ug/mL
  • Simple Western 5-20 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gelsolin/GSN Antibody (893205) [Unconjugated]

• Actin-depolymerizing factor
• ADF
• AGEL
• Brevin
• DKFZp313L0718
• gelsolin (amyloidosis, Finnish type)
• Gelsolin
• GSN

Background

Gelsolin, also known as GSN, actin-depolymerizing factor/ADF, AGEL and Brevin, is a 90-95 kDa member of the villin/gelsolin family.  Widely expressed, Gelsolin binds to actin and fibronectin, and is found both secreted in plasma and in cytoplasm.  Cytoplasmic Gelsolin lacks 51 N-terminal amino acids (aa) present in the secreted protein.  Gelsolin was identified by its ability to sever actin filaments in the presence of submicromolar calcium, and plays a role in ciliogenesis.  Defects in Gelsolin cause amyloidosis type 5, a hereditary disease characterized by gelsolin amyloid deposition.  Full-length human Gelsolin is 782 aa and shares 93% aa identity with mouse and rat Gelsolin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Gelsolin/GSN Antibody (MAB8170)(1)

Publication using MAB8170

• Z Chen, K Li, X Yin, H Li, Y Li, Q Zhang, H Wang, Y Qiu Lower Expression of Gelsolin in Colon Cancer and Its Diagnostic Value in Colon Cancer Patients J Cancer, 2019-01-30;10(5):1288-1296. 2019-01-30 [PMID: 30854138] (Neutralization, Human)

Bioinformatics

• Gene Symbol: GSN
• Uniprot:
  • P06396(Human)