FABP4/A-FABP Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse thymus tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) ...read more
FABP4 was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 10 µg/mL for 3 hours at ...read more
FABP4 was detected in immersion fixed mouse mesenchymal stem cell-derived adipocytes using 10 µg/mL Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) for 3 hours at room ...read more
Simple Western lane view shows lysates of mouse thymus tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A‑FABP at approximately 17 kDa (as indicated) using 2.5 µg/mL ...read more
FABP4 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 5 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ICC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

FABP4/A-FABP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse FABP4
Met1-Ala132
Accession # P04117
Specificity
Detects mouse FABP4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) FABP5 and rmFABP9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP4
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1443 in the following applications:

Publications
Read Publications using
AF1443 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP4/A-FABP Antibody [Unconjugated]

  • Adipocyte lipid-binding protein
  • Adipocyte-type fatty acid-binding protein
  • AFABP
  • A-FABP
  • A-FABPAFABP
  • ALBP
  • aP2
  • FABP4
  • fatty acid binding protein 4, adipocyte
  • Fatty acid-binding protein 4
  • fatty acid-binding protein, adipocyte

Background

FABP-4 (fatty acid binding protein-4; also Adipocyte-type FABP, ALBP and aP2) is a 14-15 kDa member of the fatty acid binding protein family, calycin superfamily of molecules.  It is expressed by adipocytes, macrophages, monocyte-derived DC and myoepithelial cells, and is known to serve as a carrier for select PPAR gamma fatty acid ligands as they enter the nucleus.  These ligands, or fatty acids, facilitate PPAR gamma -mediated gene transcription.  Mouse FABP-4 is 132 amino acids (aa) in length. It is a two beta -sheet molecule that contains an NLS (aa 22-32) and a fatty acid binding segment (aa 127-129).  Mouse FABP-4 is 94% and 92% aa identical to rat and human FABP-4, respectively.     

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NB600-232
Species: Hu, Mu
Applications: WB, ChIP, IHC-P, IP
MAB6799
Species: Hu
Applications: WB, IHC
NBP2-01223
Species: Hu
Applications: WB, Flow, IHC, IHC-P
AF7197
Species: Hu, Mu
Applications: WB, IHC, ICC
AF1476
Species: Hu, Mu, Rt
Applications: WB, IHC
AF3166
Species: Hu
Applications: WB, Simple Western, IHC
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
NBP1-88057
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-49533
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB400-114
Species: Hu, Mu, Rt, Po, Ch, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-31113
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-16366
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for FABP4/A-FABP Antibody (AF1443)(9)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: ICC, IHC, IHC Frozen, IP, WB.


Filter By Application
ICC
(2)
IHC
(1)
IHC Frozen
(1)
IP
(1)
WB
(4)
All Applications
Filter By Species
Mouse
(8)
Rat
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF1443 Applications Species
L Shu, RL Hoo, X Wu, Y Pan, IP Lee, LY Cheong, SR Bornstein, X Rong, J Guo, A Xu A-FABP mediates adaptive thermogenesis by promoting intracellular activation of thyroid hormones in brown adipocytes Nat Commun, 2017;8(0):14147. 2017 [PMID: 28128199] (IP,, Mouse) IP Mouse
CE Myers, DB Hoelzinger, TN Truong, LA Chew, A Myles, L Chaudhuri, JB Egan, J Liu, SJ Gendler, PA Cohen Chemotherapy can induce weight normalization of morbidly obese mice despite undiminished ingestion of high fat diet Oncotarget, 2017;8(3):5426-5438. 2017 [PMID: 28076839] (ICC,, Mouse) ICC Mouse
Epigenetic programming of Dnmt3a mediated by AP2? is required for granting preadipocyte the ability to differentiate Cell Death Dis, 2016;7(12):e2496. 2016 [PMID: 27906176] (WB,, Mouse) WB Mouse
Epigenetic programming of Dnmt3a mediated by AP2? is required for granting preadipocyte the ability to differentiate Cell Death Dis, 2016;7(12):e2496. 2016 [PMID: 27906176] (WB,, Mouse) WB Mouse
Donati G, Proserpio V, Lichtenberger B, Natsuga K, Sinclair R, Fujiwara H, Watt F Epidermal Wnt/beta-catenin signaling regulates adipocyte differentiation via secretion of adipogenic factors. Proc Natl Acad Sci U S A, 2014;111(15):E1501-9. 2014 [PMID: 24706781] (IHC,, Mouse) IHC Mouse
Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013;504(7479):277-81. 2013 [PMID: 24336287] (IHC Frozen,, Mouse) IHC Frozen Mouse
Simon B, Parlee S, Learman B, Mori H, Scheller E, Cawthorn W, Ning X, Gallagher K, Tyrberg B, Assadi-Porter F, Evans C, MacDougald O Artificial sweeteners stimulate adipogenesis and suppress lipolysis independently of sweet taste receptors. J Biol Chem, 2013;288(45):32475-89. 2013 [PMID: 24068707] (WB,, Mouse) WB Mouse
Asumda FZ, Chase PB Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol., 2011;12(0):44. 2011 [PMID: 21992089] (ICC,, Rat) ICC Rat
Ghoshal S, Trivedi DB, Graf GA, Loftin CD Cyclooxygenase-2 deficiency attenuates adipose tissue differentiation and inflammation in mice. J. Biol. Chem., 2011;286(1):889-98. 2011 [PMID: 20961858] (WB,, Mouse) WB Mouse

Review for FABP4/A-FABP Antibody (AF1443) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: ICC.

Reviews using AF1443:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC Rat 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 22364878
SpeciesRat

Blocking

Blocking DetailsSee PMID 22364878

Details

Detection NotesSee PMID 22364878

Comments

CommentsPublished See PMID 22364878

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FABP4/A-FABP Antibody (AF1443) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional FABP4/A-FABP Products

Bioinformatics Tool for FABP4/A-FABP Antibody (AF1443)

Discover related pathways, diseases and genes to FABP4/A-FABP Antibody (AF1443). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FABP4/A-FABP Antibody (AF1443)

Discover more about diseases related to FABP4/A-FABP Antibody (AF1443).
 

Pathways for FABP4/A-FABP Antibody (AF1443)

View related products by pathway.

PTMs for FABP4/A-FABP Antibody (AF1443)

Learn more about PTMs related to FABP4/A-FABP Antibody (AF1443).

Blogs on FABP4/A-FABP

There are no specific blogs for FABP4/A-FABP, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
01/05/2015
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol FABP4
Entrez
Uniprot