FABP4/A-FABP Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse thymus tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) ...read more
FABP4 was detected in immersion fixed mouse mesenchymal stem cell-derived adipocytes using 10 µg/mL Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) for 3 hours at room ...read more
FABP4 was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 10 µg/mL for 3 hours at ...read more
FABP4 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 5 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of mouse thymus tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A‑FABP at approximately 17 kDa (as indicated) using 2.5 µg/mL ...read more
A-FABP mediates expression of Dio2 via inhibition of LXR alpha .(a,b) BAT isolated from WT and A-FABP KO mice (a) fed with STC or HFD for 24 weeks or (b) subjected to room temperature (23 °C) or cold exposure (6 °C) ...read more
A-FABP accelerates proteasomal degradation of LXR alpha .(a) Primary adipocytes derived from male 6-week-old A-FABP KO mice or WT littermates were treated with actinomycin D (actD, 1 μg ml−1) or vehicle (PBS). The ...read more
NPGPx is a suppressor of adipogenesis via ROS regulationRelative NPGPx expression assayed by qRT-PCR (left panel) and Western blots (right panel) in the stromal vascular fraction (SVF) and adipocyte fraction isolated ...read more
A-FABP mediates expression of Dio2 via inhibition of LXR alpha .(a,b) BAT isolated from WT and A-FABP KO mice (a) fed with STC or HFD for 24 weeks or (b) subjected to room temperature (23 °C) or cold exposure (6 °C) ...read more
Circulating A-FABP facilitates the uptake of free fatty acid into adipocytes.(a) Circulating A-FABP and (b) FFA profile of male 4-week-old C57BL/6N mice fed with HFD for 24 weeks (n=8). (c) Circulating A-FABP and (d) ...read more
NPGPx is a suppressor of adipogenesis via ROS regulationRelative NPGPx expression assayed by qRT-PCR (left panel) and Western blots (right panel) in the stromal vascular fraction (SVF) and adipocyte fraction isolated ...read more
A-FABP accelerates proteasomal degradation of LXR alpha .(a) Primary adipocytes derived from male 6-week-old A-FABP KO mice or WT littermates were treated with actinomycin D (actD, 1 μg ml−1) or vehicle (PBS). The ...read more
Chemotherapy leads to fat loss in diet induced obese miceA. Weight of epididymal white adipose tissue (eWAT) from LFD, HFD, CY200 and MTX50 treated mice 1 week after last chemotherapy treatment. Each treatment group ...read more
In vitro differentiation capacity of young and old BM-MSCs: (A) Representative micrographs from each experimental group showing morphological change after 21 days of differentiation. (B) Results of adipogenic, ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

FABP4/A-FABP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse FABP4
Met1-Ala132
Accession # P04117
Specificity
Detects mouse FABP4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) FABP5 and rmFABP9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 2.5 ug/mL
  • Western Blot 0.25 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF1443 in the following applications:

Publications
Read Publications using
AF1443 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP4/A-FABP Antibody [Unconjugated]

  • Adipocyte lipid-binding protein
  • Adipocyte-type fatty acid-binding protein
  • AFABP
  • A-FABP
  • A-FABPAFABP
  • ALBP
  • aP2
  • FABP4
  • fatty acid binding protein 4, adipocyte
  • Fatty acid-binding protein 4
  • fatty acid-binding protein, adipocyte

Background

FABP-4 (fatty acid binding protein-4; also Adipocyte-type FABP, ALBP and aP2) is a 14-15 kDa member of the fatty acid binding protein family, calycin superfamily of molecules.  It is expressed by adipocytes, macrophages, monocyte-derived DC and myoepithelial cells, and is known to serve as a carrier for select PPAR gamma fatty acid ligands as they enter the nucleus.  These ligands, or fatty acids, facilitate PPAR gamma -mediated gene transcription.  Mouse FABP-4 is 132 amino acids (aa) in length. It is a two beta -sheet molecule that contains an NLS (aa 22-32) and a fatty acid binding segment (aa 127-129).  Mouse FABP-4 is 94% and 92% aa identical to rat and human FABP-4, respectively.     

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FABP4/A-FABP Antibody (AF1443)(23)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 7 applications: ICC, ICC/IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(7)
ICC/IF
(1)
IHC
(5)
IHC-Fr
(1)
IHC-P
(3)
Immunoprecipitation
(1)
Western Blot
(7)
All Applications
Filter By Species
Human
(2)
Mouse
(19)
Rat
(2)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using AF1443 Applications Species
Tate, T;Plumber, SA;Al-Ahmadie, H;Chen, X;Choi, W;Lu, C;Viny, A;Batourina, E;Gartensson, K;Alija, B;Molotkov, A;Wiessner, G;McKiernan, J;McConkey, D;Dinney, C;Czerniak, B;Mendelsohn, CL; Combined Mek inhibition and Pparg activation Eradicates Muscle Invasive Bladder cancer in a Mouse Model of BBN-induced Carcinogenesis bioRxiv : the preprint server for biology 2023-08-21 [PMID: 37662238] (IHC, Mouse) IHC Mouse
N Zioni, AA Bercovich, N Chapal-Ila, T Bacharach, N Rappoport, A Solomon, R Avraham, E Kopitman, Z Porat, M Sacma, G Hartmut, M Scheller, C Muller-Tid, D Lipka, E Shlush, M Minden, N Kaushansky, LI Shlush Inflammatory signals from fatty bone marrow support DNMT3A driven clonal hematopoiesis Nature Communications, 2023-04-12;14(1):2070. 2023-04-12 [PMID: 37045808] (IHC, Mouse) IHC Mouse
Z Bahrami-Ne, ZB Zhang, S Tholen, S Sharma, A Rabiee, ML Zhao, FB Kraemer, MN Teruel Early enforcement of cell identity by a functional component of the terminally differentiated state PloS Biology, 2022-12-05;20(12):e3001900. 2022-12-05 [PMID: 36469503] (Western Blot, Mouse) Western Blot Mouse
ZB Zhang, J Sinha, Z Bahrami-Ne, MN Teruel The circadian clock mediates daily bursts of cell differentiation by periodically restricting cell-differentiation commitment Proceedings of the National Academy of Sciences of the United States of America, 2022-08-08;119(33):e2204470119. 2022-08-08 [PMID: 35939672] (ICC, Mouse) ICC Mouse
AR Sathyanara, CK Lu, CC Liaw, CC Chang, HY Han, BD Green, WJ Huang, C Huang, WD He, LC Lee, HK Liu 1,2,3,4,6-Penta-O-galloyl-d-glucose Interrupts the Early Adipocyte Lifecycle and Attenuates Adiposity and Hepatic Steatosis in Mice with Diet-Induced Obesity International Journal of Molecular Sciences, 2022-04-06;23(7):. 2022-04-06 [PMID: 35409415] (Western Blot, Rat) Western Blot Rat
Q Heydt, C Xintaropou, A Clear, M Austin, I Pislariu, F Miraki-Mou, P Cutillas, K Korfi, M Calaminici, W Cawthorn, K Suchacki, A Nagano, JG Gribben, M Smith, JD Cavenagh, H Oakervee, A Castleton, D Taussig, B Peck, A Wilczynska, L McNaughton, D Bonnet, F Mardakheh, B Patel Adipocytes disrupt the translational programme of acute lymphoblastic leukaemia to favour tumour survival and persistence Nature Communications, 2021-09-17;12(1):5507. 2021-09-17 [PMID: 34535653] (ICC/IF, Human) ICC/IF Human
Y Xiao, L Shu, X Wu, Y Liu, LY Cheong, B Liao, X Xiao, RL Hoo, Z Zhou, A Xu Fatty acid binding protein-4 promotes autoimmune diabetes by recruitment and activation of pancreatic islet macrophages JCI Insight, 2021-04-08;0(0):. 2021-04-08 [PMID: 33690220] (Western Blot, Mouse) Western Blot Mouse
S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing Cell Stem Cell, 2020-08-04;27(3):396-412.e6. 2020-08-04 [PMID: 32755548] (ICC, IHC, Mouse, Transgenic Mouse) ICC, IHC Mouse, Transgenic Mouse
C Valet, A Batut, A Vauclard, A Dortignac, M Bellio, B Payrastre, P Valet, S Severin Adipocyte Fatty Acid Transfer Supports Megakaryocyte Maturation Cell Rep, 2020-07-07;32(1):107875. 2020-07-07 [PMID: 32640240] (IHC, Mouse) IHC Mouse
ML Zhao, A Rabiee, KM Kovary, Z Bahrami-Ne, B Taylor, MN Teruel Molecular Competition in G1 Controls When Cells Simultaneously Commit to Terminally Differentiate and Exit the Cell Cycle Cell Rep, 2020-06-16;31(11):107769. 2020-06-16 [PMID: 32553172] (ICC, Mouse) ICC Mouse
Show All 23 Publications.

Reviews for FABP4/A-FABP Antibody (AF1443) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using AF1443:
Filter by Applications
WB
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FABP4/A-FABP AF1443
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 11/03/2020
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Summary

ApplicationWestern Blot
Sample Testedadipose tissue cultured medium
SpeciesMouse
LotIOG0719051
  4
reviewed by:
Verified Customer
ICC Rat 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 22364878
SpeciesRat

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Recent Reviews

4
5
0
4
2
3
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2
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1
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Verified Customer
11/03/2020
Application: WB
Species: Mouse

 
Verified Customer
01/05/2015
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol FABP4
Uniprot