FABP4/A-FABP Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse thymus tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) ...read more
FABP4 was detected in immersion fixed mouse mesenchymal stem cell-derived adipocytes using 10 µg/mL Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) for 3 hours at room ...read more
FABP4 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 5 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of mouse thymus tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A‑FABP at approximately 17 kDa (as indicated) using 2.5 µg/mL ...read more
FABP4 was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 10 µg/mL for 3 hours at ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

FABP4/A-FABP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse FABP4
Met1-Ala132
Accession # P04117
Specificity
Detects mouse FABP4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) FABP5 and rmFABP9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 2.5 ug/mL
  • Western Blot 0.25 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF1443 in the following applications:

Publications
Read Publications using
AF1443 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP4/A-FABP Antibody [Unconjugated]

  • Adipocyte lipid-binding protein
  • Adipocyte-type fatty acid-binding protein
  • AFABP
  • A-FABP
  • A-FABPAFABP
  • ALBP
  • aP2
  • FABP4
  • fatty acid binding protein 4, adipocyte
  • Fatty acid-binding protein 4
  • fatty acid-binding protein, adipocyte

Background

FABP-4 (fatty acid binding protein-4; also Adipocyte-type FABP, ALBP and aP2) is a 14-15 kDa member of the fatty acid binding protein family, calycin superfamily of molecules.  It is expressed by adipocytes, macrophages, monocyte-derived DC and myoepithelial cells, and is known to serve as a carrier for select PPAR gamma fatty acid ligands as they enter the nucleus.  These ligands, or fatty acids, facilitate PPAR gamma -mediated gene transcription.  Mouse FABP-4 is 132 amino acids (aa) in length. It is a two beta -sheet molecule that contains an NLS (aa 22-32) and a fatty acid binding segment (aa 127-129).  Mouse FABP-4 is 94% and 92% aa identical to rat and human FABP-4, respectively.     

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FABP4/A-FABP Antibody (AF1443)(21)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 7 applications: ICC, ICC/IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(7)
ICC/IF
(1)
IHC
(3)
IHC-Fr
(1)
IHC-P
(3)
Immunoprecipitation
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(2)
Mouse
(16)
Rat
(2)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using AF1443 Applications Species
ZB Zhang, J Sinha, Z Bahrami-Ne, MN Teruel The circadian clock mediates daily bursts of cell differentiation by periodically restricting cell-differentiation commitment Proceedings of the National Academy of Sciences of the United States of America, 2022;119(33):e2204470119. 2022 [PMID: 35939672] (ICC, Mouse) ICC Mouse
AR Sathyanara, CK Lu, CC Liaw, CC Chang, HY Han, BD Green, WJ Huang, C Huang, WD He, LC Lee, HK Liu 1,2,3,4,6-Penta-O-galloyl-d-glucose Interrupts the Early Adipocyte Lifecycle and Attenuates Adiposity and Hepatic Steatosis in Mice with Diet-Induced Obesity International Journal of Molecular Sciences, 2022;23(7):. 2022 [PMID: 35409415] (Western Blot, Rat) Western Blot Rat
Q Heydt, C Xintaropou, A Clear, M Austin, I Pislariu, F Miraki-Mou, P Cutillas, K Korfi, M Calaminici, W Cawthorn, K Suchacki, A Nagano, JG Gribben, M Smith, JD Cavenagh, H Oakervee, A Castleton, D Taussig, B Peck, A Wilczynska, L McNaughton, D Bonnet, F Mardakheh, B Patel Adipocytes disrupt the translational programme of acute lymphoblastic leukaemia to favour tumour survival and persistence Nature Communications, 2021;12(1):5507. 2021 [PMID: 34535653] (ICC/IF, Human) ICC/IF Human
Y Xiao, L Shu, X Wu, Y Liu, LY Cheong, B Liao, X Xiao, RL Hoo, Z Zhou, A Xu Fatty acid binding protein-4 promotes autoimmune diabetes by recruitment and activation of pancreatic islet macrophages JCI Insight, 2021;0(0):. 2021 [PMID: 33690220] (Western Blot, Mouse) Western Blot Mouse
S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing Cell Stem Cell, 2020;27(3):396-412.e6. 2020 [PMID: 32755548] (IHC, Transgenic Mouse) IHC Transgenic Mouse
S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing Cell Stem Cell, 2020;27(3):396-412.e6. 2020 [PMID: 32755548] (ICC, Mouse) ICC Mouse
C Valet, A Batut, A Vauclard, A Dortignac, M Bellio, B Payrastre, P Valet, S Severin Adipocyte Fatty Acid Transfer Supports Megakaryocyte Maturation Cell Rep, 2020;32(1):107875. 2020 [PMID: 32640240] (IHC, Mouse) IHC Mouse
ML Zhao, A Rabiee, KM Kovary, Z Bahrami-Ne, B Taylor, MN Teruel Molecular Competition in G1 Controls When Cells Simultaneously Commit to Terminally Differentiate and Exit the Cell Cycle Cell Rep, 2020;31(11):107769. 2020 [PMID: 32553172] (ICC, Mouse) ICC Mouse
M Saçma, J Pospiech, R Bogeska, W de Back, JP Mallm, V Sakk, K Soller, G Marka, A Vollmer, R Karns, N Cabezas-Wa, A Trumpp, S Méndez-Fer, MD Milsom, MA Mulaw, H Geiger, MC Florian Haematopoietic stem cells in perisinusoidal niches are protected from ageing Nat. Cell Biol., 2019;21(11):1309-1320. 2019 [PMID: 31685996] (ICC, Mouse) ICC Mouse
C Liu, T Tate, E Batourina, ST Truschel, S Potter, M Adam, T Xiang, M Picard, M Reiley, K Schneider, M Tamargo, C Lu, X Chen, J He, H Kim, CL Mendelsohn Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells Nat Commun, 2019;10(1):4589. 2019 [PMID: 31597917] (IHC-P, Mouse) IHC-P Mouse
Show All 21 Publications.

Reviews for FABP4/A-FABP Antibody (AF1443) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using AF1443:
Filter by Applications
WB
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FABP4/A-FABP AF1443
Enlarge
4
reviewed by:
Zeyuan Zhang
WB Mouse 11/03/2020
View

Summary

ApplicationWestern Blot
Sample Testedadipose tissue cultured medium
SpeciesMouse
LotIOG0719051
  4
reviewed by:
Anonymous -
ICC Rat 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 22364878
SpeciesRat

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Bioinformatics Tool for FABP4/A-FABP Antibody (AF1443)

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Zeyuan Zhang
11/03/2020
Application: WB
Species: Mouse

 
Anonymous -
01/05/2015
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol FABP4
Uniprot