PPAR alpha/NR1C1 Antibody (H0723) [Unconjugated]

• Reactivity: Hu
• Applications: WB, IP, ChIP, ELISA, GS
• Clone: H0723
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Suitable Sample Type: 23005

PPAR alpha/NR1C1 Antibody (H0723) [Unconjugated] Summary

• Immunogen: Recombinant human PPAR alpha/NR1C1
  aa 4-96
• Specificity: This antibody specifically recognizes human PPAR alpha and cross-reacts with mouse PPAR alpha. This antibody does not cross-react with human PPAR gamma or PPARδ. Not yet tested in other species.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPARA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Immunoprecipitation
  • Chromatin Immunoprecipitation (ChIP)
  • Direct ELISA
  • Gel Supershift Assay

Packaging, Storage & Formulations

• Storage: This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Buffer: A liquid formulation in physiologic saline with 0.1% NaN₃.
• Preservative: 0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PPAR alpha/NR1C1 Antibody (H0723) [Unconjugated]

• alpha
• hPPAR
• NR1C1
• NR1C1MGC2237
• Nuclear receptor subfamily 1 group C member 1
• peroxisome proliferator-activated receptor alpha
• PPAR alpha
• PPARA
• PPARalpha
• PPAR-alpha
• PPARMGC2452

Background

Peroxisome Proliferator-Activated Receptor alpha (PPAR alpha ; NR1C1) is a member of the Nuclear Receptor superfamily. PPAR alpha exhibits the highest affinity with unsaturated fatty acids, and linolenic acids. PPAR alpha is expressed in brown fat, liver, kidney, heart, mucosa of the stomach and duodenum, retina, adrenal gland, skeletal muscle, pancreatic islets and smooth muscle cells. PPAR alpha plays important roles in lipid and glucose metabolism, and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease. Three members have been identified and are called PPAR alpha, δ, and lambda. Retinoid X Receptor (RXR) is an obligate partner for PPAR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPAR alpha/NR1C1 Antibody (PP-H0723-00)(4)

Publications using PP-H0723-00

• FW Wang, CH Cao, K Han, YX Zhao, MY Cai, ZC Xiang, JX Zhang, JW Chen, LP Zhong, Y Huang, SF Zhou, XH Jin, XY Guan, RH Xu, D Xie APC-activated long non-coding RNA inhibits colorectal carcinoma pathogenesis through reducing exosome production J. Clin. Invest., 2018;0(0):. 2018 [PMID: 30511962] (ChIP, Human)
• Lien S, Wei S, Chang S, Chang M, Chang J, Chiu J Activation of PPAR-alpha induces cell cycle arrest and inhibits transforming growth factor-beta1 induction of smooth muscle cell phenotype in 10T1/2 mesenchymal cells. Cell Signal, 2013;25(5):1252-63. 2013 [PMID: 23385087] (Western Blot, Mouse)
• Zhou J, Wang KC, Wu W, Subramaniam S, Shyy JY, Chiu JJ, Li JY, Chien S MicroRNA-21 targets peroxisome proliferators-activated receptor-alpha in an autoregulatory loop to modulate flow-induced endothelial inflammation. Proc. Natl. Acad. Sci. U.S.A., 2011;108(25):10355-60. 2011 [PMID: 21636785] (Western Blot, Human)
• Huang W, Rha GB, Han MJ, Eum SY, Andras IE, Zhong Y, Hennig B, Toborek M PPARalpha and PPARgamma effectively protect against HIV-induced inflammatory responses in brain endothelial cells. J. Neurochem., 2008;107(2):497-509. 2008 [PMID: 18710415] (Western Blot, Human)

FAQs for PPAR alpha/NR1C1 Antibody (PP-H0723-00). (Showing 1 - 1 of 1 FAQs).

1. Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two
   • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.

Bioinformatics

• Gene Symbol: PPARA