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PPAR delta/NR1C2 Antibody

Images

 
Western Blot: PPAR delta/NR1C2 Antibody [NBP2-22468] - Analysis of 25 ug of NIH-3T3 (Lane 1), PC12 (Lane 2), and K562 (Lane 3) cell lysates and a molecular weight protein ladder.
Flow Cytometry: PPAR delta/NR1C2 Antibody [NBP2-22468] - Analysis of PPAR delta was done on jurkat cells. Cells were fixed with 70% ethanol for 10 minutes permeablized with 0.25% Triton (R) X-100 for 20 minutes and ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PPAR delta/NR1C2 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues M(1) E Q P Q E E T P E A R E E(14) C of mouse PPAR delta.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPARD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 3 - 5 ug / 10^6 cells
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:250 - 1:750
Application Notes
WB: Detects an approx. 49 kDa protein representing PPAR delta from NIH-3T3 cell lysate. Immunolocalization yeilds predominant nuclear staining.
Publications
Read Publications using
NBP2-22468 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PPAR delta/NR1C2 Antibody

  • FAAR
  • MGC3931
  • NR1C2
  • NR1C2PPAR-delta
  • NUC1
  • NUC1PPAR-beta
  • NUCI
  • NUCII
  • Nuclear hormone receptor 1
  • Nuclear receptor subfamily 1 group C member 2
  • peroxisome proliferative activated receptor, delta
  • Peroxisome proliferator-activated receptor beta
  • peroxisome proliferator-activated receptor delta
  • PPAR delta
  • PPARB
  • PPARD

Background

The Peroxisome Proliferator Activated Receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. There are 3 known subtypes of PPARs, PPAR alpha, PPAR delta and PPAR gamma. PPAR gamma is believed to be involved in adipocyte differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PPAR delta/NR1C2 Antibody (NBP2-22468)(4)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PPAR delta/NR1C2 Antibody (NBP2-22468) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPARD
Entrez