PPAR delta/NR1C2 Antibody

Western Blot: PPAR delta/NR1C2 Antibody [NBP2-22468] - Analysis of 25 ug of NIH-3T3 (Lane 1), PC12 (Lane 2), and K562 (Lane 3) cell lysates and a molecular weight protein ladder.

Flow Cytometry: PPAR delta/NR1C2 Antibody [NBP2-22468] - Analysis of PPAR delta was done on jurkat cells. Cells were fixed with 70% ethanol for 10 minutes permeablized with 0.25% Triton (R) X-100 for 20 minutes and blocked with 5% BSA for 30 minutes at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PPAR delta/NR1C2 Antibody Summary

• Description: Novus Biologicals Rabbit PPAR delta/NR1C2 Antibody (NBP2-22468) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-PPAR delta/NR1C2 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues M(1) E Q P Q E E T P E A R E E(14) C of mouse PPAR delta.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPARD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 3 - 5 ug / 10^6 cells
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:250 - 1:750
• Application Notes: WB: Detects an approx. 49 kDa protein representing PPAR delta from NIH-3T3 cell lysate. Immunolocalization yeilds predominant nuclear staining.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPAR delta/NR1C2 Antibody

• FAAR
• MGC3931
• NR1C2
• NR1C2PPAR-delta
• NUC1
• NUC1PPAR-beta
• NUCI
• NUCII
• Nuclear hormone receptor 1
• Nuclear receptor subfamily 1 group C member 2
• peroxisome proliferative activated receptor, delta
• Peroxisome proliferator-activated receptor beta
• peroxisome proliferator-activated receptor delta
• PPAR delta
• PPARB
• PPARD

Background

The Peroxisome Proliferator Activated Receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. There are 3 known subtypes of PPARs, PPAR alpha, PPAR delta and PPAR gamma. PPAR gamma is believed to be involved in adipocyte differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPAR delta/NR1C2 Antibody (NBP2-22468)(4)

Publications using NBP2-22468

• Wu J, Wang X, Zhang M et al. RXR Agonists Enhance Lenalidomide Anti-Myeloma Activity and T Cell Functions while Retaining Glucose-Lowering Effect Cells 2023-08-03 [PMID: 37566072] (Immunohistochemistry-Frozen, Mouse)
• Wu J, Chu E, Paul B, Kang Y Mechanistic Studies and a Retrospective Cohort Study: The Interaction between PPAR Agonists and Immunomodulatory Agents in Multiple Myeloma Cancers 2022-10-27 [PMID: 36358696] (WB, Human)
• Sha Y, Wu J, Paul B et al. PPAR agonists attenuate lenalidomide's anti-myeloma activity in vitro and in vivo Cancer letters 2022-07-21 [PMID: 35872263]
• Burda JE, O'Shea TM, Ao Y et al. Divergent transcriptional regulation of astrocyte reactivity across disorders Nature 2022-05-25 [PMID: 35614216] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: PPARD
• Entrez:
  • 25682(Rat)