POM121 Antibody

Western Blot: POM121 Antibody [NBP2-19890] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with POM121 antibody [N2N3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: POM121 Antibody [NBP2-19890] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using POM121 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:500.

Immunohistochemistry-Paraffin: POM121 Antibody [NBP2-19890] - HBL435 xenograft, using POM121 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: POM121 Antibody [NBP2-19890] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

POM121 Antibody Summary

• Description: Novus Biologicals Rabbit POM121 Antibody (NBP2-19890) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-POM121 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human POM121. The exact sequence is proprietary.
• Localization: Nucleus; nuclear pore complex; Nucleus membrane; Single-pass membrane protein; Endoplasmic reticulum membrane; Single-pass membrane protein (By similarity)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POM121
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 128 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for POM121 Antibody

• FLJ41820
• MGC3792
• nuclear envelope pore membrane protein POM 121
• nuclear pore membrane protein 121 kDa
• nucleoporin Nup121
• NUP121
• P145
• POM121 membrane glycoprotein
• POM121A

Background

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POM121 Antibody (NBP2-19890)(6)

Publications using NBP2-19890

• Stang TE, Salapa HE, Clarke JWE et al. Heterogeneous Nuclear Ribonucleoprotein A1 Knockdown Alters Constituents of Nucleocytoplasmic Transport Brain Sciences 2024-10-19 [PMID: 39452051]
• Bilir S, Kojidani T, Mori C et al. Roles of Nup133, Nup153 and membrane fenestrations in assembly of the nuclear pore complex at the end of mitosis Genes Cells 2019-02-28 [PMID: 30821042] (Human)
• Kim Jihye, Chung Joon-Yong, Kim Tae-Joong et al. Genomic Network-Based Analysis Reveals Pancreatic Adenocarcinoma Up-Regulating Factor-Related Prognostic Markers in Cervical Carcinoma. Frontiers in Oncology 2018-10-23 [PMID: 30406031] (IHC-P, Human)
• Guo J, Liu X, Wu C et al. The transmembrane nucleoporin Pom121 ensures efficient HIV-1 pre-integration complex nuclear import. Virology. 2018-06-25 [PMID: 29957337] (WB, Human)
• Rossi A, Coum A, Madelenat M, et al. Neural stem cells alter nucleocytoplasmic partitioning and accumulate nuclear polyadenylated transcripts during quiescence bioRxiv 2021-01-07 (WB)
• Hochmair J, Exner C, Franck M et al. Molecular crowding and RNA synergize to promote phase separation, microtubule interaction, and seeding of Tau condensates The EMBO journal 2022-03-17 [PMID: 35298090] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: POM121
• Uniprot:
  • Q96HA1(Human)