HGFR/c-MET Antibody [Unconjugated]

Images

 
HGF R/c-MET was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) overnight at 4 °C. Tissue ...read more
HGF R/c‑MET was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive control, left panel) and U937 human histiocytic lymphoma cell line (negative control, right panel) using Goat ...read more
HGF R/c-MET was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) at 10 µg/mL overnight at 4 °C. ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HGF R/c-Met knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

HGFR/c-MET Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human HGF R/c-MET
Glu25-Thr932
Accession # P08581
Specificity
Detects human HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse HGF R is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MET
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
Control
HGFR/c-MET Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
AF276 in the following applications:

Publications
Read Publications using
AF276 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HGFR/c-MET Antibody [Unconjugated]

  • AUTS9
  • cMET
  • c-MET
  • EC 2.7.10
  • EC 2.7.10.1
  • hepatocyte growth factor receptor
  • HGF R
  • HGF receptor
  • HGF/SF receptor
  • HGFR
  • Met (c-Met)
  • met proto-oncogene (hepatocyte growth factor receptor)
  • met proto-oncogene tyrosine kinase
  • MET
  • oncogene MET
  • Proto-oncogene c-Met
  • RCCP2
  • Scatter factor receptor
  • SF receptor
  • Tyrosine-protein kinase Met

Background

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains (5-7). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 8). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (9, 10). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (11). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha 6/ beta 4, Plexins B1, 2, 3, and MSP R/Ron (12-19). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (12-19). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (12, 16, 17). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (20). Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, human HGF R shares 86-88% aa sequence identity with canine, mouse, and rat HGF R.

  1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
  2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
  4. Park, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:6379.
  5. Crepaldi, T. et al. (1994) J. Biol. Chem. 269:1750.
  6. Prat, M. et al. (1991) Mol. Cell. Biol. 12:5954.
  7. Rodrigues, G.A. et al. (1991) Mol. Cell. Biol. 11:2962.
  8. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
  9. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
  10. Ponzetto, C. et al. (1994) Cell 77:261.
  11. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
  12. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
  13. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
  14. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
  15. Wang, X. et al. (2002) Mol. Cell 9:411.
  16. Trusolino, L. et al. (2001) Cell 107:643.
  17. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
  18. Conrotto, P. et al. (2004) Oncogene 23:5131.
  19. Follenzi, A. et al. (2000) Oncogene 19:3041.
  20. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HGFR/c-MET Antibody (AF276)(17)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: Bioassay, ELISA Development (Detection), ICC, IHC-P, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
ELISA Development (Detection)
(2)
ICC
(3)
IHC-P
(4)
Neutralization
(3)
Western Blot
(2)
All Applications
Filter By Species
Human
(16)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF276 Applications Species
R Graves-Dea, G Bogatcheva, S Rehman, Y Lu, JN Higginboth, B Singh Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer Oncotarget, 2019;10(13):1320-1333. 2019 [PMID: 30863492] (Western Blot, Human) Western Blot Human
D Novo, N Heath, L Mitchell, G Caligiuri, A MacFarlane, D Reijmer, L Charlton, J Knight, M Calka, E McGhee, E Dornier, D Sumpton, S Mason, A Echard, K Klinkert, J Secklehner, F Kruiswijk, K Vousden, IR Macpherson, K Blyth, P Bailey, H Yin, LM Carlin, J Morton, S Zanivan, JC Norman Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels Nat Commun, 2018;9(1):5069. 2018 [PMID: 30498210] (Bioassay, Human) Bioassay Human
JB Xiao, XL Li, L Liu, G Wang, SN Hao, HJ Dong, XM Wang, YF Zhang, HD Liu The association of semaphorin 5A with lymph node metastasis and adverse prognosis in cervical cancer Cancer Cell Int., 2018;18(0):87. 2018 [PMID: 29977159] (Neutralization, Human) Neutralization Human
Rachel Barrow-McG Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes Nat Commun, 2016;7(0):11942. 2016 [PMID: 27336951]
Deborah R Kaye Tumor and Plasma Met Levels in Non-Metastatic Prostate Cancer PLoS ONE, 2016;11(6):e0157130. 2016 [PMID: 27300295] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling. Nat Commun, 2015;6(0):6776. 2015 [PMID: 25873032] (IHC-P, Human) IHC-P Human
Tabernero J, Elez M, Herranz M, Rico I, Prudkin L, Andreu J, Mateos J, Carreras M, Han M, Gifford J, Credi M, Yin W, Agarwal S, Komarnitsky P, Baselga J A pharmacodynamic/pharmacokinetic study of ficlatuzumab in patients with advanced solid tumors and liver metastases. Clin Cancer Res, 2014;20(10):2793-804. 2014 [PMID: 24634378] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Hasenauer S, Malinger D, Koschut D, Pace G, Matzke A, von Au A, Orian-Rousseau V Internalization of Met requires the co-receptor CD44v6 and its link to ERM proteins. PLoS ONE, 2013;8(4):e62357. 2013 [PMID: 23626807] (ICC, Human) ICC Human
Muller, P A J, Trinidad, A G, Timpson, P, Morton, J P, Zanivan, S, van den Berghe, P V E, Nixon, C, Karim, S A, Caswell, P T, Noll, J E, Coffill, C R, Lane, D P, Sansom, O J, Neilsen, P M, Norman, J C, Vousden, K H Mutant p53 enhances MET trafficking and signalling to drive cell scattering and invasion. Oncogene, 2013;32(10):1252-65. 2013 [PMID: 22580601] (Western Blot, Human) Western Blot Human
Cho K, Park J, Park C, Lee D, Lee E, Kim D, Kim K, Yoon S, Park Y, Kim E, Cho S, Jang S, Park B, Chi S, Yoo S, Jang M, Kim H, Kim E, Jo K, Park Y Identification of a pivotal endocytosis motif in c-Met and selective modulation of HGF-dependent aggressiveness of cancer using the 16-mer endocytic peptide. Oncogene, 2012;32(8):1018-29. 2012 [PMID: 22525273] (Human) Human
Show All 17 Publications.

Review for HGFR/c-MET Antibody (AF276) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF276:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22897854
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22897854

Details

Detection NotesSee PMID 22897854

Comments

CommentsPublished: See PMID 22897854

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Anonymous
01/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol MET
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