HGFR/c-MET Antibody [Unconjugated]


HGF R/c-MET was detected in immersion fixed HT-29 human colon adenocarcinoma cell line (positive control, left panel) and U937 human histiocytic lymphoma cell line (negative control, right panel) using Goat Anti-Human ...read more
HGF R/c-MET was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) at 10 µg/mL overnight at 4 °C. ...read more
HGF R/c-MET was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) overnight at 4 °C. Tissue ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HGF R/c-Met knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HGFR/c-MET at approximately 151 kDa (as indicated) using 25 µg/mL of Goat ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, B/N, CyTOF-ready, ICC/IF, KO
Validated by:

Genetic Strategies


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HGFR/c-MET Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human HGF R/c-MET
Accession # P08581
Detects human HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse HGF R is observed.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 25 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
AF276 in the following applications:

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AF276 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HGFR/c-MET Antibody [Unconjugated]

  • AUTS9
  • cMET
  • c-MET
  • EC 2.7.10
  • EC
  • hepatocyte growth factor receptor
  • HGF R
  • HGF receptor
  • HGF/SF receptor
  • HGFR
  • Met (c-Met)
  • met proto-oncogene (hepatocyte growth factor receptor)
  • met proto-oncogene tyrosine kinase
  • MET
  • oncogene MET
  • Proto-oncogene c-Met
  • RCCP2
  • Scatter factor receptor
  • SF receptor
  • Tyrosine-protein kinase Met


HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains (5-7). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 8). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (9, 10). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (11). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha 6/ beta 4, Plexins B1, 2, 3, and MSP R/Ron (12-19). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (12-19). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (12, 16, 17). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (20). Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, human HGF R shares 86-88% aa sequence identity with canine, mouse, and rat HGF R.

  1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
  2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
  4. Park, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:6379.
  5. Crepaldi, T. et al. (1994) J. Biol. Chem. 269:1750.
  6. Prat, M. et al. (1991) Mol. Cell. Biol. 12:5954.
  7. Rodrigues, G.A. et al. (1991) Mol. Cell. Biol. 11:2962.
  8. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
  9. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
  10. Ponzetto, C. et al. (1994) Cell 77:261.
  11. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
  12. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
  13. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
  14. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
  15. Wang, X. et al. (2002) Mol. Cell 9:411.
  16. Trusolino, L. et al. (2001) Cell 107:643.
  17. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
  18. Conrotto, P. et al. (2004) Oncogene 23:5131.
  19. Follenzi, A. et al. (2000) Oncogene 19:3041.
  20. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HGFR/c-MET Antibody (AF276)(26)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 8 applications: Bioassay, ELISA Capture, ELISA Development (Detection), Flow Cytometry, ICC, IHC-P, Neutralization, Western Blot.

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ELISA Development (Detection)
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Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using AF276 Applications Species
K Koo, A Wuenschman, A Rendahl, KY Song, C Forster, A Wolf-Ringw, A Borgatti, A Giubellino Expression and Prognostic Evaluation of the Receptor Tyrosine Kinase MET in Canine Malignant Melanoma Veterinary sciences, 2023-03-26;10(4):. 2023-03-26 [PMID: 37104404] (Western Blot, Canine) Western Blot Canine
Zuo Q, Yang Y, Lyu Y, Yang C, Chen C, Salman S, Huang T, Wicks E, Jackson W, Datan E, Qin W, Semenza G Plexin-B3 expression stimulates MET signaling, breast cancer stem cell specification, and lung metastasis. Cell Rep, 2023-02-28;42(3):112164. 2023-02-28 [PMID: 36857181] (Western Blot, Human) Western Blot Human
BS Mesquita, MHAM Fens, A Di Maggio, EDC Bosman, WE Hennink, M Heger, S Oliveira The Impact of Nanobody Density on the Targeting Efficiency of PEGylated Liposomes International Journal of Molecular Sciences, 2022-11-29;23(23):. 2022-11-29 [PMID: 36499301] (Flow Cytometry, Human) Flow Cytometry Human
Q Zhang, DK Jeppesen, JN Higginboth, R Graves-Dea, VQ Trinh, MA Ramirez, Y Sohn, AC Neininger, N Taneja, ET McKinley, H Niitsu, Z Cao, R Evans, SE Glass, KC Ray, WH Fissell, S Hill, KL Rose, WJ Huh, MK Washington, GD Ayers, DT Burnette, S Sharma, LH Rome, JL Franklin, YA Lee, Q Liu, RJ Coffey Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Nature Cell Biology, 2021-12-09;23(12):1240-1254. 2021-12-09 [PMID: 34887515] (Western Blot, Human) Western Blot Human
P Banerjee, GY Xiao, X Tan, VJ Zheng, L Shi, MNB Rabassedas, HF Guo, X Liu, J Yu, L Diao, J Wang, WK Russell, J Roszik, CJ Creighton, JM Kurie The EMT activator ZEB1 accelerates endosomal trafficking to establish a polarity axis in lung adenocarcinoma cells Nature Communications, 2021-11-03;12(1):6354. 2021-11-03 [PMID: 34732702] (ICC, Human) ICC Human
M Nászai, K Bellec, Y Yu, A Román-Fern, E Sandilands, J Johansson, AD Campbell, JC Norman, OJ Sansom, DM Bryant, JB Cordero RAL GTPases mediate EGFR-driven intestinal stem cell proliferation and tumourigenesis Elife, 2021-06-07;10(0):. 2021-06-07 [PMID: 34096503] (ELISA Capture, Human) ELISA Capture Human
C Fedeli, H Moreno, S Kunz The Role of Receptor Tyrosine Kinases in Lassa Virus Cell Entry Viruses, 2020-08-06;12(8):. 2020-08-06 [PMID: 32781509] (Flow Cytometry, Human) Flow Cytometry Human
GY Xiao, SL Schmid FCHSD2 controls oncogenic ERK1/2 signaling outcome by regulating endocytic trafficking PLoS Biol., 2020-07-17;18(7):e3000778. 2020-07-17 [PMID: 32678845] (Flow Cytometry, Human) Flow Cytometry Human
R Graves-Dea, G Bogatcheva, S Rehman, Y Lu, JN Higginboth, B Singh Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer Oncotarget, 2019-02-12;10(13):1320-1333. 2019-02-12 [PMID: 30863492] (Western Blot, Human) Western Blot Human
D Novo, N Heath, L Mitchell, G Caligiuri, A MacFarlane, D Reijmer, L Charlton, J Knight, M Calka, E McGhee, E Dornier, D Sumpton, S Mason, A Echard, K Klinkert, J Secklehner, F Kruiswijk, K Vousden, IR Macpherson, K Blyth, P Bailey, H Yin, LM Carlin, J Morton, S Zanivan, JC Norman Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels Nat Commun, 2018-11-29;9(1):5069. 2018-11-29 [PMID: 30498210] (Bioassay, Human) Bioassay Human
Show All 26 Publications.

Review for HGFR/c-MET Antibody (AF276) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF276:
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reviewed by:
Anonymous -
WB Human 01/07/2015


ApplicationWestern Blot
Sample TestedSee PMID 22897854

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Recent Reviews


Anonymous -
Application: WB
Species: Human


Gene Symbol MET