SHC1 Antibody (OTI3A1)

Images

 
Western Blot: SHC1 Antibody (OTI3A1) [NBP2-02434] - Figure from citation:(p66shc) protein level by using anti-SHC1 antibody in human HBL-100/MCF-7 cell lysates.
Immunocytochemistry/ Immunofluorescence: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SHC1 .
Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded prostate tissue using anti-SHC1 mouse monoclonal antibody.
Western Blot: SHC1 Antibody (OTI3A1) [NBP2-02434] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded bladder tissue using anti-SHC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded breast tissue using anti-SHC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-SHC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-SHC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded colon tissue using anti-SHC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded Kidney tissue using anti-SHC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded lung tissue using anti-SHC1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
OTI3A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

SHC1 Antibody (OTI3A1) Summary

Immunogen
Full length human recombinant protein of human SHC1 (NP_003020) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SHC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation
  • Western Blot 1:2000
Theoretical MW
51.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SHC1 Antibody (OTI3A1)

  • FLJ26504
  • p66
  • p66SHC
  • SH2 domain protein C1
  • SHC (Src homology 2 domain containing) transforming protein 1
  • SHC (Src homology 2 domain-containing) transforming protein 1
  • SHC
  • SHC1
  • SHCA
  • SHC-transforming protein 1
  • SHC-transforming protein 3
  • SHC-transforming protein A
  • Src homology 2 domain-containing-transforming protein C1

Background

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SHC1 Antibody (NBP2-02434). (Showing 1 - 1 of 1 FAQ).

  1. I have just received a SHC1 antibody, and can’t find the concentration on the label. Could you please provide the concentration? The lot # is A01.
    • The concentration for NBP2-02434 Lot A01 is 1.0mg/ml.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SHC1
Entrez