POLR2D Antibody

Western Blot: POLR2D Antibody [NBP2-93656] - Analysis of 200ug extracts of HepG2 cells using POLR2D at a dilition of 1:1000.

Immunocytochemistry/Immunofluorescence: POLR2D Antibody [NBP2-93656] - Analysis of A549 cells using POLR2D .

Western Blot: POLR2D Antibody [NBP2-93656] - Analysis of extracts of various cell lines, using POLR2D at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

POLR2D Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR2D
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:100
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for POLR2D Antibody

• DNA-directed RNA polymerase II 16 kDa polypeptide
• DNA-directed RNA polymerase II subunit D
• DNA-directed RNA polymerase II subunit RPB4
• HSRBP4
• HSRPB4
• polymerase (RNA) II (DNA directed) polypeptide D
• RBP4
• RNA polymerase II 16 kDa subunit
• RNA polymerase II subunit B4
• RNA polymerase II subunit hsRBP4
• RPB16

Background

The POLR2D gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2D