POLD3 Antibody - BSA Free

Western Blot: POLD3 Antibody [NBP2-93032] - Analysis of extracts of various cell lines, using POLD3 . Exposure time: 60s.

Immunocytochemistry/Immunofluorescence: POLD3 Antibody [NBP2-93032] - Analysis of U2OS cells using POLD3 .

Immunocytochemistry/Immunofluorescence: POLD3 Antibody [NBP2-93032] - Analysis of GFP-RNF168 transgenic U2OS cells using POLD3 can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Immunohistochemistry: POLD3 Antibody - BSA Free [NBP2-93032] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using POLD3 Rabbit pAb at dilution of 1:300 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

POLD3 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 197-466 of human POLD3 (NP_006582.1). ASKAAAKTQETNKETKTEAKEVTNASAAGNKAPGKGNMMSNFFGKAAMNKFKVNLDSEQAVKEEKIVEQPTVSVTEPKLATPAGLKKSSKKAEPVKVLQKEKKRGKRVALSDDETKETENMRKKRRRIKLPESDSSEDEVFPDSPGAYEAESPSPPPPPSPPLEPVPKTEPEPPSVKSSSGENKRKRKRVLKSKTYLDGEGCIVTEKVYESESCTDSEEELNMKTSSVHRPPAMTVKKEPREERKGPKKGTAALGKANRQVSITGFFQRK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLD3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for POLD3 Antibody - BSA Free

• DNA polymerase delta subunit 3
• DNA polymerase delta subunit p66
• DNA polymerase delta, subunit 3
• KIAA0039MGC119643
• MGC119642
• P66
• P68
• polymerase (DNA directed), delta 3
• polymerase (DNA-directed), delta 3, accessory subunit

Background

PolD3/p66 is a subunit of the heterotetrameric DNA polymerase delta that plays a role in DNA replication and repair. The PolD3/p66 subunit has been shown to mediate the interaction between DNA polymerase delta and PCNA (proliferating cell nuclear antigen) to facilitate processivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLD3