PLK1 Antibody

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Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunocytochemistry/ Immunofluorescence: PLK1 Antibody [NB100-547] - Asynchronous HeLa cells. Antibody used at 0.5 ug/ml (left panel) and 0.2 ug/ml (right panel). Cells were counterstained with DAPI.
Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 on HeLa whole cell lysate using NB100-547. B. Affinity purified rabbit anti-PLK1 antibodies NB100-546, NB100-547, and NB100-548. IPed PLK1 was detected ...read more
Immunocytochemistry/ Immunofluorescence: PLK1 Antibody [NB100-547] - NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-PLK1 used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at ...read more
Immunocytochemistry/ Immunofluorescence: PLK1 Antibody [NB100-547] - NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-PLK1 used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at ...read more
Proximity Ligation Assay: PLK1 Antibody [NB100-547] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM6 (NB100-251) and rabbit anti-human PLK1 (NB100-547). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: PLK1 Antibody [NB100-547] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM7 (NB100-252) and rabbit anti-human PLK1 (NB100-547). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: PLK1 Antibody [NB100-547] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RAD17 (NB100-272) and rabbit anti-human PLK1 (NB100-547). Image merged from DAPI (2ms) and Texas ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

PLK1 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residues 325 and 375 of human Polo-like kinase using the numbering given in SwissProt entry P53350 (GeneID 5347).
Marker
Mitosis Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:500-1:2000
  • Immunoprecipitation 2-4 ug/mg lysate
  • Proximity Ligation Assay 1:1000-1:10000
Application Notes
For IHC: Permeabilization with Triton-X 100 is recommended.
Positive Control
HeLa Lysate (NB800-PC1)
PLK1 Lysate (NBL1-14515)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PLK1 Antibody

  • cell cycle regulated protein kinase
  • EC 2.7.11
  • EC 2.7.11.21
  • PLK
  • PLK1
  • PLK-1
  • polo (Drosophia)-like kinase
  • polo like kinase
  • polo-like kinase 1polo-like kinase (Drosophila)
  • Serine/threonine-protein kinase 13
  • serine/threonine-protein kinase PLK1
  • STPK13

Background

Polo-like kinases are a family of serine/threonine protein kinases, named after the prototypic founding member of the family, the polo gene product of Drosophila melanogaster. The polo kinase was originally identified in mutants that display abnormal mitotic spindle organization. Subsequently, potential homologues of Drosophila polo have been identified in yeasts (Cdc5p in Saccharomyces cerevisiae; plo1+ in Schizosaccharmoyces pombe) and in mammals (polo-like kinase 1; Plk1). Genetic and biochemical studies suggest that polo, Cdc5p and plo1+ may be required for mitotic spindle organization and, possibly, for cytokinesis. Likewise, the patterns of expression, activity and subcellular localization of Plk1 suggest that Plk1 may function during mitosis in spindle assembly and function. Recent studies have demonstrated that mammalian Plks regulate the function of the Golgi complex, a cellular organelle closely associated with the centrosome and also have microtubule organization activity. Furthermore, deregulated expression of human PLK1 and PLK3 is strongly correlated with the development of many types of malignancies, and ectopic expression of kinase-active Plk3 or Plk1 dominant negative protein leads to rapid cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLK1
Entrez
Uniprot