PLK1 Antibody - BSA Free

Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunocytochemistry/Immunofluorescence: PLK1 Antibody [NB100-547] - Detection of human PLK1 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- PLK1 (NB100-547). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated.

Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 on HeLa whole cell lysate using NB100-547. B. Affinity purified rabbit anti-PLK1 antibodies NB100-546, NB100-547, and NB100-548. IPed PLK1 was detected using NB100-548.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PLK1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PLK1 Antibody - BSA Free (NB100-547) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-PLK1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residues 325 and 375 of human Polo-like kinase using the numbering given in SwissProt entry P53350 (GeneID 5347).
• Marker: Mitosis Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500-1:2000
  • Immunoprecipitation 2-4 ug/mg lysate
  • Western Blot 1:1000-1:10000
• Application Notes: Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Control:

  HeLa Whole Cell Lysate
  PLK1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PLK1 Antibody - BSA Free

• cell cycle regulated protein kinase
• EC 2.7.11
• EC 2.7.11.21
• PLK
• PLK1
• PLK-1
• polo (Drosophia)-like kinase
• polo like kinase
• polo-like kinase 1polo-like kinase (Drosophila)
• Serine/threonine-protein kinase 13
• serine/threonine-protein kinase PLK1
• STPK13

Background

Polo-like kinases are a family of serine/threonine protein kinases, named after the prototypic founding member of the family, the polo gene product of Drosophila melanogaster. The polo kinase was originally identified in mutants that display abnormal mitotic spindle organization. Subsequently, potential homologues of Drosophila polo have been identified in yeasts (Cdc5p in Saccharomyces cerevisiae; plo1+ in Schizosaccharmoyces pombe) and in mammals (polo-like kinase 1; Plk1). Genetic and biochemical studies suggest that polo, Cdc5p and plo1+ may be required for mitotic spindle organization and, possibly, for cytokinesis. Likewise, the patterns of expression, activity and subcellular localization of Plk1 suggest that Plk1 may function during mitosis in spindle assembly and function. Recent studies have demonstrated that mammalian Plks regulate the function of the Golgi complex, a cellular organelle closely associated with the centrosome and also have microtubule organization activity. Furthermore, deregulated expression of human PLK1 and PLK3 is strongly correlated with the development of many types of malignancies, and ectopic expression of kinase-active Plk3 or Plk1 dominant negative protein leads to rapid cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLK1 Antibody (NB100-547)(3)

Publications using NB100-547

• Carden S, Vitiello E, Rosa E Silva I, Holder J et Al. Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method Dev Cell 2023-10-18 [PMID: 37852252]
• Bremner Sk A role for AMPK in the regulation of mitosis and cytokinesis Thesis 2020-03-01 (ICC/IF, IB, Mouse, Human)
• McDougall W M, Perreira J M et al. Viral Infection or IFN-Alplha Alters Mitotic Spindle Orientation by Modulating Pericentrin Levels. iScience 2019-02-22 [PMID: 30716700] (WB, Human)

Bioinformatics

• Gene Symbol: PLK1
• Entrez:
  • 5347(Human)
  • 18817(Mouse)
• Uniprot:
  • P53350()