PLK4 Antibody

Images

 
Western Blot: PLK4 Antibody [NBP1-33402] - A: H1299.
Immunocytochemistry/ Immunofluorescence: PLK4 Antibody [NBP1-33402] - Methanol-fixed HeLa, using PLK4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2000.
Immunohistochemistry-Paraffin: PLK4 Antibody [NBP1-33402] - Paraffin-embedded human colon cancer diluted at 1:500.
Immunohistochemistry-Paraffin: PLK4 Antibody [NBP1-33402] - MCF7 xenograft.
Immunohistochemistry-Paraffin: PLK4 Antibody [NBP1-33402] - Human endometrial cancer: Paraffin-embedded human endometrial cancer diluted at 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.6 mg/ml

Order Details

PLK4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human PLK4. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLK4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
109 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.6 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PLK4 Antibody

  • EC 2.7.11.21
  • PLK-4
  • polo-like kinase 4 (Drosophila)
  • polo-like kinase 4SAKSTK18serine/threonine kinase 18
  • Sak
  • serine/threonine protein kinase SAK
  • Serine/threonine-protein kinase 18
  • serine/threonine-protein kinase PLK4
  • Serine/threonine-protein kinase Sak
  • Snk akin kinase

Background

This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PLK4 Antibody (NBP1-33402) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLK4