PKM1 Antibody - BSA Free

Images

 
Western Blot: PKM1 Antibody [NBP2-14833] - Expression profile of PKM isoforms in tissues from mouse organs. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions as Figure 1a at the ...read more
Immunohistochemistry-Paraffin: PKM1 Antibody [NBP2-14833] - PKM1 antibody was tested in human breast cancer using DAB with hematoxylin counterstain.
Western Blot: PKM1 Antibody [NBP2-14833] - PKM1 antibody was tested in 1. human skeletal muscle 2. mouse skeletal muscle 3. human brain 4. mouse brain and 5. human heart cell lysate.
Western Blot: PKM1 Antibody [NBP2-14833] - Detection of PKM1 on HEK293T and U-87 cells. Lane 1: HEK293T cells (major isoform is PKM2, human). Lane 2: HEK293T cells with plasmid overexpressing mouse PKM1. Lane 3: HEK293T ...read more
Biological Strategies: Western Blot: PKM1 Antibody [NBP2-14833] - Expression of PTB1, PKM1, and PKM2 proteins at 72 h after transfection of DLD-1, RD or KYM-1 cells with miR-133b (10, 20 nM). Image collected and ...read more
Western Blot: PKM1 Antibody [NBP2-14833] - Expression profile of PKM isoforms in various cancer cell lines, control cell lines, and cells in primary culture. PKM1 and PKM2 were detected by Western blotting under the ...read more
Simple Western: PKM1 Antibody [NBP2-14833] - Simple Western lane view shows a specific band for PKM1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.17 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

PKM1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human PKM1 protein (between residues 350-450) [UniProt P14618-2].
Localization
Cytoplasm.
Specificity
This antibody is specific for the M1 isoform of PKM.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PKM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting reported in scientific literature (PMID 25416385)
  • Immunocytochemistry/ Immunofluorescence reported in multiple pieces of scientific literature
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Simple Western 1:400
  • Western Blot 0.5 ug/ml
Application Notes
In Western blot a band is seen ~57 kDa in human and mouse skeletal muscle, brain and heart. In IHC-P, staining was observed in the cytoplasm of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Mouse Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 3 Reviews rated 5
using
NBP2-14833 in the following applications:

Publications
Read Publications using
NBP2-14833 in the following applications:

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 25416385).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.17 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKM1 Antibody - BSA Free

  • PK1
  • Pyruvate kinase isozyme M1
  • Pyruvate kinase isozymes M1
  • Pyruvate kinase M1

Background

Pyruvate kinases are glycolytic enzymes that transfer a phosphate group from phosphoenolpyruvate to ADP generating pyruvate and ATP. There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues as well as in most cancer cells. The M1 isoform differs from the M2 isoform due to a change in sequence between residues 389-433. In the presence of D-fructose 1,6-bisphosphate (FBP) the monomer reversibly forms the highly active tetrameric form. This tetramer is associated with the glycolytic enzyme complex and activates the pyruvate kinase activity. A nearly inactive dimeric form is associated with tumor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PKM1 Antibody (NBP2-14833)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Bovine.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IF/IHC
(2)
IHC-Fr
(1)
IHC-P
(2)
WB
(14)
All Applications
Filter By Species
Human
(9)
Mouse
(6)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NBP2-14833 Applications Species
Li Y, Chen X, Huang H et al. A feedback loop between NONHSAT024276 and PTBP1 inhibits tumor progression and glycolysis in HCC by increasing the PKM1/PKM2 ratio Cancer Science 2023-04-01 [PMID: 36529521] (WB) WB
Deck M, Van Hameren G, Campbell G et al. Physiology of PNS axons relies on glycolytic metabolism in myelinating Schwann cells PloS one 2022-10-04 [PMID: 36194565] (WB, IHC-Fr, Mouse) WB, IHC-Fr Mouse
Han J, Hyun J, Park J et al. Aberrant role of pyruvate kinase M2 in the regulation of gamma-secretase and memory deficits in Alzheimer's disease Cell reports 2021-12-07 [PMID: 34879266]
Tian, L, Xiong, P Y Et al. Supra-coronary aortic banding improves right ventricular function in experimental pulmonary arterial hypertension in rats by increasing systolic right coronary artery perfusion. Acta Physiol (Oxf) 2020-08-01 [PMID: 32339403] (ICC/IF, Human) ICC/IF Human
Okazaki Mitsuyoshi, Fushida Sachio, Tsukada Tomoya et al. The effect of HIF-1a and PKM1 expression on acquisition of chemoresistance. Cancer Management and Research 2018-01-01 [PMID: 30013393] (WB, IF/IHC, Mouse) WB, IF/IHC Mouse
Shanmugasundaram K, Nayak BK, Friedrichs WE et al. NOX4 functions as a mitochondrial energetic sensor coupling cancer metabolic reprogramming to drug resistance. Nat Commun. 2017-10-19 [PMID: 29051480] (WB, Human) WB Human
Zhang H, Wang D et al. Metabolic and Proliferative State of Vascular Adventitial Fibroblasts in Pulmonary Hypertension Is Regulated Through a MicroRNA-124/PTBP1 (Polypyrimidine Tract Binding Protein 1)/Pyruvate Kinase Muscle Axis. Circulation 2017-12-19 [PMID: 28972001] (WB, Bovine) WB Bovine
Minami K, Taniguchi K, Sugito N et al. MiR-145 negatively regulates Warburg effect by silencing KLF4 and PTBP1 in bladder cancer cells. Oncotarget. 2017-05-16 [PMID: 28380435] (WB, Human) WB Human
Takai T, Yoshikawa Y, Inamoto T et al. A Novel Combination RNAi toward Warburg Effect by Replacement with miR-145 and Silencing of PTBP1 Induces Apoptotic Cell Death in Bladder Cancer Cells Int J Mol Sci 2017-01-17 [PMID: 28106737] (WB, Human) WB Human
Sugiyama T, Taniguchi K, Matsuhashi N et al. MiR-133b inhibits growth of human gastric cancer cells by silencing pyruvate kinase muscle-splicer polypyrimidine tract-binding protein 1. Cancer Sci. 2016-12-01 [PMID: 27696637] (WB, Human) WB Human
Show All 18 Publications.

Reviews for PKM1 Antibody (NBP2-14833) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP2-14833:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot PKM1 NBP2-14833
Enlarge
5
reviewed by:
Min-Joon Han
WB Human 10/24/2018
View

Summary

ApplicationWestern Blot
Sample TestedHuman fibroblast
SpeciesHuman
LotA-3
  5
reviewed by:
Verified Customer
WB Human 03/31/2016
View

Summary

ApplicationWestern Blot
Sample TestedHCT116 cell line lysate
SpeciesHuman
LotA-2
Western Blot PKM1 NBP2-14833
Enlarge
5
reviewed by:
Corey .
WB Human 12/10/2012
View

Summary

ApplicationWestern Blot
Sample TestedU-87 cells with PTB/A1/A2 shRNAs
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKM1 Antibody (NBP2-14833). (Showing 1 - 1 of 1 FAQs).

  1. I just wanted to make sure the L/R didn't hit M1 and M2. I do not need to distinguish between L and R. So do you have an L/R specific monoclonal antibody for western blot?
    • We do have an antibody that is specific for PKM1, however it is a polyclonal. The catalog number is NBP2-14833.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional PKM1 Products

Array NBP2-14833

Diseases for PKM1 Antibody (NBP2-14833)

Discover more about diseases related to PKM1 Antibody (NBP2-14833).
 

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Blogs on PKM1

There are no specific blogs for PKM1, but you can read our latest blog posts.
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Contact Information

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Min-Joon Han
10/24/2018
Application: WB
Species: Human

 
Verified Customer
03/31/2016
Application: WB
Species: Human

Corey .
12/10/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol PKM
Entrez
Uniprot