PKM1 Antibody [Alexa Fluor™ Plus 488]

• Reactivity: Hu, Mu, Bv
• Applications: WB, ICC/IF, IHC, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 488

PKM1 Antibody [Alexa Fluor™ Plus 488] Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human PKM1 protein (between residues 350-450) [UniProt P14618-2].
• Localization: Cytoplasm.
• Specificity: This antibody is specific for the M1 isoform of PKM.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PKM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 25416385).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PKM1 Antibody [Alexa Fluor™ Plus 488]

• PK1
• Pyruvate kinase isozymes M1
• Pyruvate kinase M1

Background

Pyruvate kinases are glycolytic enzymes that transfer a phosphate group from phosphoenolpyruvate to ADP generating pyruvate and ATP. There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues as well as in most cancer cells. The M1 isoform differs from the M2 isoform due to a change in sequence between residues 389-433. In the presence of D-fructose 1,6-bisphosphate (FBP) the monomer reversibly forms the highly active tetrameric form. This tetramer is associated with the glycolytic enzyme complex and activates the pyruvate kinase activity. A nearly inactive dimeric form is associates with tumor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PKM