Phosphoribosyl Pyrophosphate Amidotransferase Antibody

Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody [NBP2-19812] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7.5% SDS PAGE diluted at 1:1000

Immunocytochemistry/Immunofluorescence: Phosphoribosyl Pyrophosphate Amidotransferase Antibody [NBP2-19812] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Phosphoribosyl Pyrophosphate Amidotransferase Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Phosphoribosyl Pyrophosphate Amidotransferase. The exact sequence is proprietary.
• Predicted Species: Mouse (94%), Rat (91%), Rhesus Macaque (100%), Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPAT
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Phosphoribosyl Pyrophosphate Amidotransferase Antibody

• ATASE
• EC 2.4.2.14
• glutamine phosphoribosylpyrophosphatate amidotransferase
• Glutamine phosphoribosylpyrophosphate amidotransferase
• glutamine PRPP amidotransferase
• GPATamidophosphoribosyltransferase
• phosphoribosyl pyrophosphate amidotransferase
• PRAT

Background

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PPAT
• Uniprot:
  • Q06203(Human)