Western Blot: ADE2 Antibody [NBP1-90235] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Simple Western: ADE2 Antibody [NBP1-90235] - Simple Western lane view shows a specific band for ADE2 in 0.2 mg/ml of RT-4 and U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: ADE2 Antibody [NBP1-90235] - Electropherogram image(s) of corresponding Simple Western lane view. ADE2 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).
Hu, Mu, RtSpecies Glossary
WB, Simple Western, IHC, IHC-P
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2) and 40% Glycerol
0.02% Sodium Azide
Immunogen affinity purified
Western Blot 1:100 - 1:250
Simple Western 1:20
Immunohistochemistry-Paraffin 1:50 - 1:200
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.