Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free

Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Phosphoribosyl Pyrophosphate Amidotransferase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Staining of paraffin-embedded Human Kidney tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.

Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-Phosphoribosyl Pyrophosphate Amidotransferase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: hum

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Staining of paraffin-embedded Human colon tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Staining of paraffin-embedded Human liver tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, IHC
• Clone: OTI1B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 42-278 of human PPAT(NP_002694) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPAT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 57.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free

• ATASE
• EC 2.4.2.14
• glutamine phosphoribosylpyrophosphatate amidotransferase
• Glutamine phosphoribosylpyrophosphate amidotransferase
• glutamine PRPP amidotransferase
• GPATamidophosphoribosyltransferase
• phosphoribosyl pyrophosphate amidotransferase
• PRAT

Background

Phosphoribosyl Pyrophosphate Amidotransferase is encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPAT