Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8)

Images

 
Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B8) [NBP2-02056] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Phosphoribosyl Pyrophosphate ...read more
Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B8) [NBP2-02056] - Staining of paraffin-embedded Human liver tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse ...read more
Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B8) [NBP2-02056] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Phosphoribosyl Pyrophosphate Amidotransferase monoclonal ...read more
Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B8) [NBP2-02056] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Phosphoribosyl Pyrophosphate ...read more
Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B8) [NBP2-02056] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Phosphoribosyl Pyrophosphate ...read more
Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B8) [NBP2-02056] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Phosphoribosyl Pyrophosphate ...read more
Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B8) [NBP2-02056] - Staining of paraffin-embedded Human colon tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse ...read more
Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B8) [NBP2-02056] - Staining of paraffin-embedded Human Kidney tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clone
OTI1B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 42-278 of human PPAT(NP_002694) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PPAT
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
57.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-02056.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8)

  • ATASE
  • EC 2.4.2.14
  • glutamine phosphoribosylpyrophosphatate amidotransferase
  • Glutamine phosphoribosylpyrophosphate amidotransferase
  • glutamine PRPP amidotransferase
  • GPATamidophosphoribosyltransferase
  • phosphoribosyl pyrophosphate amidotransferase
  • PRAT

Background

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Phosphoribosyl Pyrophosphate Amidotransferase Antibody (NBP2-02056)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73394AF350
Alexa Fluor 405 NBP2-73394AF405
Alexa Fluor 488 NBP2-73394AF488
Alexa Fluor 532 NBP2-73394AF532
Alexa Fluor 594 NBP2-73394AF594
Alexa Fluor 647 NBP2-73394AF647
Alexa Fluor 700 NBP2-73394AF700
Alexa Fluor 750 NBP2-73394AF750
Biotin NBP2-73394B
DyLight 350 NBP2-73394UV
DyLight 405 NBP2-73394V
DyLight 488 NBP2-73394G
DyLight 550 NBP2-73394R
DyLight 594 NBP2-73394DL594
DyLight 650 NBP2-73394C
DyLight 680 NBP2-73394FR
DyLight 755 NBP2-73394IR
FITC NBP2-73394F
HRP NBP2-73394H
Janelia Fluor 549 NBP2-73394JF549
Janelia Fluor 646 NBP2-73394JF646

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Bioinformatics Tool for Phosphoribosyl Pyrophosphate Amidotransferase Antibody (NBP2-02056)

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Bioinformatics

Gene Symbol PPAT
Entrez