Phenylalanine Hydroxylase Antibody - BSA Free

Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Analysis in human liver and pancreas tissues. Corresponding Phenylalanine Hydroxylase RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human cerebral cortex, kidney, liver and pancreas using Anti-Phenylalanine Hydroxylase antibody NBP1-80917 (A) shows similar protein distribution across tissues to independent antibody NBP2-48615 (B).

Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Analysis using Anti-PAH antibody NBP1-80917 (A) shows similar pattern to independent antibody NBP2-48615 (B).

Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.

Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Phenylalanine Hydroxylase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Phenylalanine Hydroxylase Antibody - BSA Free (NBP1-80917) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPWFPRTIQELDRFANQILSYGAELDADHPG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAH
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Phenylalanine Hydroxylase Protein (NBP1-80917PEP)

Reactivity Notes

Mouse (86%), Rat (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Phenylalanine Hydroxylase Antibody - BSA Free

• EC 1.14.16
• EC 1.14.16.1
• PH
• phe-4-monooxygenase
• phenylalanine 4-monooxygenase
• phenylalanine hydroxylase
• phenylalanine-4-hydroxylase
• PKU
• PKU1

Background

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAH
• Entrez:
  • 5053(Human)
• Uniprot:
  • P00439(Human)